Changes in Superoxide Dismutase Activity and Peroxynitrite Content in Rat Peritoneal Macrophages Exposed to He-Ne Laser Radiation

Abstract

The formation of reactive oxygen and nitrogen species by rat peritoneal macrophages induced by a low-intensity He-Ne laser radiation (LR) was studied in this work. It was found that the formation of reactive oxygen species, nitric oxide, and peroxynitrite as well as changes in the activity of superoxide dismutase (SOD) depended to a large extent on the LR dose. In particular, it was found that activation of SOD at low LR doses was accompanied by nitric oxide level increase, while the level of peroxynitrite showed no significant changes. On the other hand, an enhanced LR dose inhibited the enzyme, and this was accompanied by peroxynitrite accumulation. All the measurements were carried out the day after LR treatment. The revealed regularities consequently demonstrate the existence of a deferred LR action on macrophages associated with the production of reactive oxygen and nitrogen species.

Irradiation with 780 nm diode laser attenuates inflammatory cytokines but upregulates nitric oxide in lipopolysaccharide‐stimulated macrophages: Implications for the prevention of aneurysm progression

“…Previous in vitro studies by Klebanov's group [27] showed that HeNe laser irradiation increased NO secretion from macrophages to a maximum of 80% with 0.6 J/cm 2 [26], and 75% with 0.36 J/cm 2 . These results are compatible with the %increase in NO secretion found in the current study (60-100%).…”

Influence of Laser (λ670 nm) and Dexamethasone on the Chronology of Cutaneous Repair

“…The collagen deposition in irradiated animals and the decrease of mononuclear cells on days 3 through 5 after wounding could be explained by the capacity of the laser locally to increase enzyme activity of mononuclear cells and fibroblasts. 8,[21][22][23] On days 5 through 7, a predominance of fusiform cells showing smooth muscle actin-a and desmin was observed by electron microscopy in animals exposed to LLLT. We assume that a light-source stimulus not only increased the number of myofibroblasts on the wound, but also activated secretory functions.…”

Low-power light and isolated rat hearts after ischemia of myocardium

“…The resulting reduction of the pH-level in the myocardium tissues inhibits SOD activity. Low-intensive He-Ne laser radiation enhances SOD activity in vitro 17,18 . Relatively wide band luminescent red light (λ peak = 630 nm) could cause the similar effects.…”

“…Ischemia of heart muscle causes dramatic changes of the functional activity of myocardium 12 , damages of membranes of cardiac myocytes [13][14][15] and the increase of LPO level in the heart tissues [14][15][16] . At the same time, it is well known 17,18 that red laser light can reactivate the SOD enzyme and control activity of the lipid peroxidation processes in the sample tissues. These phenomena might be considered as the key mechanisms of the observed photo therapeutic effect of red light on living tissues.…”