Irradiation with 780 nm diode laser attenuates inflammatory cytokines but upregulates nitric oxide in lipopolysaccharide‐stimulated macrophages: Implications for the prevention of aneurysm progression

Gavish, Lilach; Perez, Louise; Reissman, Petachia; Gertz, S. David

doi:10.1002/lsm.20635

Cited by 85 publications

(69 citation statements)

References 28 publications

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“…This speculation is due to the fact that similar results have been recently reported in a study that showed an increase in network formation with cellular migration stimulating the formation of neurons at the dose of 6.7 J/cm 2 [56], L. Gavish et al (2008) [57] irradiated monocytes/macrophages and found a decrease in IL-1β, IL-10, IL-1α, IL-6 secretion, demonstrating efficacy in the attenuation of inflammatory processes. In osteosarcoma cultures, irradiation with laser at 780 nm (1, 5, and 10 J/cm 2 ) promoted cellular proliferation without altering the ALP synthesis, which demonstrates that this cell type responds to LLLT.…”

Section: Discussionsupporting

confidence: 74%

In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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The aim of this study was to evaluate the metabolism of odontoblast-like MDPC-23 cells subjected to direct LLL irradiation. The cells were seeded (20,000 cells/well) in 24-well plates and incubated for 24 hours at 37• C. After this period, the culture medium (DMEM) was replaced by fresh DMEM supplemented with 2 or 5% (stress induction by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to laser doses of 2, 4, 10, 15 and 25 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780±3 nm, 40 mW). One control group (sham irradiation) was established for each experimental condition (laser dose x FBS supplementation). Three and 72 hours after the last irradiation, cells were analyzed with respect to metabolism, morphology, total protein expression and alkaline phosphatase (ALP) activity. Higher metabolism and total protein expression were observed 72 hours after the last irradiation at the doses of 15 and 25 J/cm 2 (Mann-Whitney; p<0.05). Higher ALP activity was obtained with 5% FBS when the cells were irradiated with doses of 2 and 10 J/cm 2 . For the dose of 25 J/cm 2 , the highest ALP activity was observed with 10% FBS. It was concluded that the LLLT parameters used in this study stimulated the metabolic activity of the MDPC-23 cells, especially at the doses of 15 and 25 J/cm 2 .

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Section: Discussionsupporting

confidence: 74%

In vitro effect of low-level laser on odontoblast-like cells

et al. 2010

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“…12 Their studies have shown that LLLT modifies some fundamental aortic aneurysm processes, progressively increasing matrix protein and smooth muscle cell proliferation, as well as secretion and expression of matrix metalloproteinase. Inhibition of gene expression of proinflammatory cytokine interleukin-1β from these cells was also observed.…”

Section: Introductionmentioning

confidence: 99%

Ação da terapia com laser de baixa potência nas fases iniciais do reparo tecidual: princípios básicos

Piva¹,

Abreu

Silva

et al. 2011

An. Bras. Dermatol.

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O objetivo do estudo foi realizar uma revisão de literatura a respeito da terapia com laser de baixa potência e sua relação com as fases iniciais de reparo. Foram analisados 22 artigos, observou-se a utilização de diferentes doses e comprimentos de ondas (632,8 a 904 nm). Nos estudos in vitro foram utilizadas doses entre 2,2 e 16 J/cm². A dose de 5 J/cm² tem sido apontada como responsável por mudanças significativas in vitro, porém a dose de 16 J/cm² promove efeito inibitório sobre o crescimento celular em culturas. Em estudos in vivo, envolvendo animais foram utilizadas doses entre 0,04 a 21 J/cm². Para estudos em humanos foram utilizadas doses entre 1,8 a 16 J/cm². Conclui-se que a terapia com laser de baixa potência exerce efeitos antiinflamatórios importantes nos processos iniciais da cicatrização: redução de mediadores químicos, de citocinas, do edema, diminuição da migração de células inflamatórias e incremento de fatores de crescimento contribuindo diretamente para o processo de reabilitação tecidual. Porém, a falta de padronização dificulta a escolha de parâmetros ideais. Keywords: Fibroblast growth factors; Inflammation; Inflammation mediators; Laser therapy, Low-level; Lasers Resumo: O objetivo do estudo foi revisar a literatura a respeito da terapia com laser de baixa potência e sua relação com as fases iniciais de reparo. Foram analisados 22 artigos, observando-se a utilização de diferentes doses e comprimentos de ondas (632,8 a 904 nm). Nos estudos in vitro, foram utilizadas doses entre 2,2 e 16 J/cm². A dose de 5 J/cm² tem sido apontada como responsável por mudanças significativas in vitro; porém, a dose de 16 J/cm² promove efeito inibitório sobre o crescimento celular em culturas. Em estudos in vivo, envolvendo animais, foram utilizadas doses entre 0,04 a 21 J/cm². Para estudos em humanos, foram utilizadas doses entre 1,8 a 16 J/cm². Conclui-se que a terapia com laser de baixa potência exerce efeitos anti-inflamatórios importantes nos processos iniciais da cicatrização: redução de mediadores químicos, de citocinas, do edema, diminuição da migração de células inflamatórias e incremento de fatores de crescimento, contribuindo diretamente para o processo de reabilitação tecidual. Porém, a falta de padronização dificulta a escolha de parâmetros ideais. Palavras-chave: Fatores de crescimento de fibroblastos; Inflamação; Lasers; Mediadores da inflamação; Terapia a laser de baixa intensidade

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“…Furthermore, our data taken together confirm previous reports and clinical trials from other laboratories that NIR laser indeed has effects on reducing inflammation. [29][30][31][32] There is evidence that light acts on cells via the NF-kB pathway among others, 16,33 and many reports have demonstrated that light could have different impacts on different cells and tissues, specifically as regards inflammation. Various studies have reported that the immediate effects of light can be regarded in some cases as pro-inflammatory 34 and immunostimulatory, 35 and in other cases as anti-inflammatory 36 and immunosuppressive.…”

mentioning

confidence: 99%

Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells

Chen

Huang

Sharma

et al. 2011

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to Investigate the effect of 810-nm low level laser therapy (LLLT) on dendritic cells (DC) in vitro. Background data: LLLT can enhance wound healing and increase cell proliferation and survival, and is used to treat inflammatory conditions. However there are reports that LLLT can stimulate leukocytes and could therefore be pro-inflammatory. Recently, DC have been found to play an important role in inflammation and immune response. Methods: Murine bone-marrow-derived DC were isolated, stimulated with lipopolysaccharide (LPS) or CpG oligodeoxynucleotide and treated with 810-nm laser, using fluences of 0.3, 3, and 30 J/cm 2 delivered at irradiances of 1, 10, and 100 mW/cm 2 respectively. Confocal microscopy, flow cytometry for DC markers, viability using propidium iodide, enzyme-linked immunosorbent assays (ELISA) for secreted interleukin-12 (IL-12), and bioluminescence measurements in cells transduced with a reporter for toll-like receptor (TLR)-9/nuclear factor kappa B (NF-kB) activation, were performed. Results: LLLT changed the morphology of LPS-stimulated DC, increased their viability, and altered the balance of DC activation markers (major histocompatibility complex [MHC] class 2 up and CD86 down). LLLT reduced IL-12 secretion from DC stimulated by either LPS or CpG. LLLT reduced NF-kB activation in reporter cells stimulated with CpG. There was no obvious light dose response observed. Conclusions: Taken together, these data suggest that 810-nm LLLT has an anti-inflammatory effect on activated DC, possibly mediated by cyclic adenosine monophosphate (cAMP) and reduced NF-kB signaling.

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Irradiation with 780 nm diode laser attenuates inflammatory cytokines but upregulates nitric oxide in lipopolysaccharide‐stimulated macrophages: Implications for the prevention of aneurysm progression

Abstract: These properties of LLLI, with its effects on smooth muscle cells reported previously, may be of profound therapeutic relevance for arterial diseases such as aneurysm where inflammatory processes and weakening of the matrix structure of the arterial wall are major pathologic components.

Cited by 85 publications

References 28 publications

In vitro effect of low-level laser on odontoblast-like cells

In vitro effect of low-level laser on odontoblast-like cells

Ação da terapia com laser de baixa potência nas fases iniciais do reparo tecidual: princípios básicos

Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells

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