Changes in the 2-DE protein profile during zygotic embryogenesis in the Brazilian Pine (Araucaria angustifolia)

Balbuena, Tiago Santana; Silveira, Vanildo; Junqueira, Magno; Dias, Leonardo Lucas Carnevalli; Santa–Catarina, Claudete; Shevchenko, Andrej; Floh, Eny Iochevet Segal

doi:10.1016/j.jprot.2009.01.011

Cited by 64 publications

(56 citation statements)

References 57 publications

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“…The characterization of profiles and changes in protein content during seed development allows for comparing in vitro and in vivo embryo development. However, although several embryogenically expressed proteins have been identified so far, there have been few reports focused on woody plants (Marsoni et al 2008), even less so regarding proteomic characterization throughout recalcitrant seed development (Pawlowski et al 2007;Silveira et al 2008;Balbuena et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

Dias

Balbuena

Silveira

et al. 2010

Braz. J. Plant Physiol.

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The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism and storage synthesis. These findings contribute to a better understanding of protein metabolism during seed development in recalcitrant seeds, besides providing information on established markers that could be useful in defining and improving somatic embryogenesis protocols, besides monitoring the development of somatic embryos in this species.Key words: 2-DE; comparative proteomics; embryo development; storage protein; Lauraceae resumo O objetivo deste trabalho foi a caracterização dos perfis protéicos expressos durante o desenvolvimento da semente de O. catharinensis, uma espécie recalcitrante, via eletroforese bidimensional. A extração de proteínas foi realizada utilizando tampão uréia/tiouréia seguido de uma etapa de precipitação com TCA 10%. A análise comparativa durante o desenvolvimento da semente mostrou um grande número de proteínas exclusivas em cada estádio do desenvolvimento. O estádio cotiledonar, que representa a fase de transição entre a embriogênese e o começo do metabolismo relacionado a maturação, apresentou o maior número de proteínas estádio-específicas. Proteínas relacionadas ao metabolismo oxidativo e a síntese de reservas foram identificadas via MS/ MS. Estes resultados contribuem para o melhor entendimento do metabolismo de proteínas durante o desenvolvimento de sementes recalcitrantes, além de prover informações no estabelecimento de parâmetros para protocolos de embriogênese somática. palavras-chave: 2-DE; desenvolvimento embrionário; Lauraceae; proteômica comparativa; proteínas de reserva Braz.

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Section: Introductionmentioning

confidence: 99%

Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

Dias

Balbuena

Silveira

et al. 2010

Braz. J. Plant Physiol.

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“…Thus, techniques for conservation and sustainable use have been investigated to encourage preservation and marketing during out of the production season periods (Amarante et al, 2007;Stahl et al, 2007;dos Santos et al,2008;Balbuena et al, 2009).…”

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confidence: 99%

Biological activities and chemical constituents of Araucaria angustifolia : An effort to recover a species threatened by extinction

Peralta

Koehnlein

Oliveira

et al. 2016

Trends in Food Science & Technology

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“…However, the recently developed de novo cross-species protein identification strategy has made it possible to conduct proteomic studies in nonmodel species with limited genomic resources (46). This strategy has been successfully applied to a variety of eukaryotic life forms (e.g., pine [4], gastropod [48], and fungi [9]), but to our knowledge it has been applied to only one eukaryotic alga, Dunaliella salina, before extensive genomic resources were available for this species (28), and in the salinity tolerance study of D. salina, de novo cross-species protein identification strategy resulted in the identification of more than twice as many proteins as the MASCOT method and with higher confidence.…”

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confidence: 99%

Characterization of the Proteomic Profiles of the Brown Tide Alga Aureoumbra lagunensis under Phosphate- and Nitrogen-Limiting Conditions and of Its Phosphate Limitation-Specific Protein with Alkaline Phosphatase Activity

Sun

Qiu

et al. 2012

Appl Environ Microbiol

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The persistent bloom of the brown tide alga Aureoumbra lagunensis has been reported in coastal embayments along southern Texas, but the molecular mechanisms that sustain such algal bloom are unknown. We compared the proteome and physiological parameters of A. lagunensis grown in phosphate (P)-depleted, P-and nitrogen (N)-depleted, and nutrient-replete cultures. For the proteomic analysis, samples from three conditions were subjected to two-dimensional electrophoresis and tandem mass spectrometry analysis. Because of the paucity of genomic resources in this species, a de novo cross-species protein search was used to identify the differentially expressed proteins, which revealed their involvement in several key biological processes, such as chlorophyll synthesis, antioxidative protection, and protein degradation, suggesting that A. lagunensis may adopt intracellular nutrient compensation, extracellular organic nutrient regeneration, and damage protection to thrive in P-depleted environments. A highly abundant P limitation-specific protein, tentatively identified as a putative alkaline phosphatase, was further characterized by enzyme activity assay on nondenaturing gel and confocal microscopy, which confirmed that this protein has alkaline phosphatase activity, is a cytoplasmic protein, and is closely associated with the cell membrane. The abundance, location, and functional expression of this alkaline phosphatase all indicate the importance of organic P utilization for A. lagunensis under P limitation and the possible role of this alkaline phosphatase in regenerating phosphate from extra-or intracellular organic phosphorus.

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Changes in the 2-DE protein profile during zygotic embryogenesis in the Brazilian Pine (Araucaria angustifolia)

Cited by 64 publications

References 57 publications

Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species

Biological activities and chemical constituents of Araucaria angustifolia : An effort to recover a species threatened by extinction

Characterization of the Proteomic Profiles of the Brown Tide Alga Aureoumbra lagunensis under Phosphate- and Nitrogen-Limiting Conditions and of Its Phosphate Limitation-Specific Protein with Alkaline Phosphatase Activity

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