Changes in the Inhibition of Specific Agglutination by Plasma Due to Microvesicles Released from Human Red Cells during Storage for Transfusion

Cole, Wendy F.; Rumsby, Martin G.; Longster, G. H.; Tovey, L. A. D.

doi:10.1111/j.1423-0410.1979.tb02273.x

Cited by 11 publications

(1 citation statement)

References 9 publications

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“…They demonstrated that when the intracellular Ca 2+ concentration was increased by treatment with A23187, a bivalent cation ionophore, there was a shape change from discocytes to echinocytes, the 1,2‐diacylglycerol content of the membranes increased, and microvesicles budded from the microvillous‐like projections of the echinocytes. These vesicles were depleted in phosphatidylcholine, spectrin, actin (band 5), and glycophorin, and band 3 and acetylcholinesterase (AChE) were retained 34,58‐61 …”

Section: Rbc Vesiculationmentioning

confidence: 99%

The how and why of exocytic vesicles

Greenwalt¹

2005

Transfusion

183

156

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The purpose of this review is to draw the attention of general readers to the importance of cellular exocytic vesiculation as a normal mechanism of development and subsequent adjustment to changing conditions, focusing on red cell (RBC) vesiculation. Recent studies have emphasized the possible role of these microparticles as diagnostic and investigative tools. RBCs lose membrane, both in vivo and during ex vivo storage, by the blebbing of microvesicles from the tips of echinocytic spicules. Microvesicles shed by RBCs in vivo are rapidly removed by the reticuloendothelial system. During storage, this loss of membrane contributes to the storage lesion and the accumulation of the microvesicles are believed to be thrombogenic and thus to be clinically important.

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Section: Rbc Vesiculationmentioning

confidence: 99%

The how and why of exocytic vesicles

Greenwalt¹

2005

Transfusion

183

156

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Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Gliesing

Stibenz

et al. 1984

Histochemistry

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The conformational state of the glycocalyx of the intact and altered erythrocyte membrane was studied by means of the topo-optical toluidine blue reaction, i.e. induced membrane birefringence. High membrane anisotropy represents the normal glycocalyx structure and its decline represents their perturbation. The results show that the glycocalyx structure is changed during ageing of the erythrocytes in vivo as well as in vitro. During fluid preservation, in vitro ageing and vesiculation of cells in vitro, a subpopulation of cells showed a decline of membrane anisotropy, but other cells demonstrated abnormally high values. In the latter cases, there is usually a correlation to spherocytes. From this point of view, it is to be assumed that spherogenesis during cell ageing is induced by cell vesiculation. This leads to a remodelling of an intact plasmalemma. In contrast, the cell fractions which are probably non-vesiculating seem to be more or less damaged by membrane and/or plasmic hydrolases. This can be mimicked by neuraminidase and protease treatment of erythrocytes in vitro. Membrane lesions caused by freeze preservation of red blood cells are rare. The topo-optical results are interpreted according to the assumptions of the theory of membrane anisotropy, i.e. the formation of dye-stuff micelles at distinct, clustered, sialylated carbohydrate chains of the glycophorin A.

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