Changes in the production and the effect of nitric oxide with aging in articular cartilage: An experimental study in rabbits

Im, Gun‐Il; Shin, Sung-Il

doi:10.1080/000164702317281332

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…In both the fibroblastic cultures and chondrocytic chondrocyte cultures, TGF-β1 seemed to induce a further wound enlargement. This may indicate that TGF-β1 may play a part in inducing a stress response in chondrocytic cells similar to that described by Lin and Helmke (2009) and a production of nitric oxide, consequently induction in cell death [13] as described by Im and Shin (2002). This may play a part in the down regulation of chondrocyte mitogenesis.…”

Section: Wound Repairmentioning

confidence: 60%

Effect of Transforming Growth Factor-β1 in Biological Regulation of Primary Chondrocyte

Khaghani

Denyer²,

Youseffi³

2012

AJBE

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The effect of transforming growth factors-β (TGF-βs) in the regulation and control of cell growth is widely studied, but the capability of these cytokines to regulate chondrocyte cell functions such as migration, cell death, proliferation, differentiation and wound repair is not clearly understood. In this work the effect of TGF-β1 on the biological regulation of chondrocyte was evaluated using a model wound closure assay. The experiments were carried out on primary chondrocyte cells with fibroblast like and chondrocytic phenotypes. The cells were isolated from knee articular cartilage of five day old Sprague-Dawley neonate rats and seeded at low density to obtain a fibroblast like morphology. Chondrocytes with chondrocytic phenotype were derived by seeding at high density.The results revealed that TGF-β1 slowed down proliferation and migration of cells into a model wound. It was also found that cell attachment, as determined by the detachment time during trypsinization, was greater for cells with a fibroblast like morphology when compared with cells exhibiting chondrocytic morphology. Treatment with TGF-β1 was found to increase the detachment times of fibroblast like chondrocytes, indicating that TGF-β1enhanced cell attachment of this cell type, whilst treatment with TGF-β1 decreased detachment time for the chondrocytic type chondrocyte cells indicating that TGF-β1 decreased cell attachment in these cells.

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Section: Wound Repairmentioning

confidence: 60%

Effect of Transforming Growth Factor-β1 in Biological Regulation of Primary Chondrocyte

Khaghani

Denyer²,

Youseffi³

2012

AJBE

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“…The mechanically scratched monolayers had damaged/injured chondrocytes in all four different flasks. Damaged chondrocytes release nitric oxide (NO) (Im and Shin, 2002) which is a highly reactive free radical that has been implicated in tissue injury in OA (Dinarello, 1996). NO has also been known to exert detrimental effects on chondrocyte function, causing inhibition of collagen and procollagen synthesis (Selvi et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Concentrations of (R)-(+)-WIN 55,212-2 on Wound Healing of Chondrocytes Monolayer.

Abdeldayem

Yousseffi²,

Khaghani

et al. 2011

International Conference on Aerospace Sciences and Aviation Tec

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Number of studies has been conducted to highlight the anti-inflammatory and immunosuppressive properties of cannabinoids and also their potentials for prevention of cartilage degradation. Various wound healing techniques can be used to investigate the mechanisms of chondrocyte repair in monolayer or 3 dimensional tissue. Therefore, we investigated the effect of two different concentrations (0.6 and 0.9μg/ml) of the synthetic cannabinoid R (+) WIN55, 212-2 on the wound healing of chondrocyte monolayers using a simple scratch assay model. WIN55, 212-2 was found to increase both the migration and proliferation of chondrocytes cultured in a chondrogenic media, with increase in the wound closure rate. These findings highlight the potential use of the synthetic cannabinoid for improving the rate of wound closure as well as acting as an anti-inflammatory agent, which could be used to enhance tissue engineering protocols aimed at cartilage repair.

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“…As can be seen from the graph in Figure 8, the wound width created on control culture is zero after 48 hours, as compared to the wound width of the TGF-β3 culture showing only 60% closure and no sign of complete wound closure, even after 48 hours. Slowing down the chondrocytes proliferation by TGF-β3 (See Table 2 for detail) might be a reason for incomplete wound closure (Takahashi, 2000, Im, 2002. High levels of nitric oxide in wound edge produced by damaged cells could be another cause of increase in cell death and consequently slowing down of the wound repair process.…”

Section: Wound-healing Assaymentioning

confidence: 99%

Effect of transforming growth factor-β3 on mono and multilayer chondrocytes

et al. 2016

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Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGFβs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provide fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.

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Changes in the production and the effect of nitric oxide with aging in articular cartilage: An experimental study in rabbits

Cited by 3 publications

References 13 publications

Effect of Transforming Growth Factor-β1 in Biological Regulation of Primary Chondrocyte

Effect of Transforming Growth Factor-β1 in Biological Regulation of Primary Chondrocyte

The Effect of Different Concentrations of (R)-(+)-WIN 55,212-2 on Wound Healing of Chondrocytes Monolayer.

Effect of transforming growth factor-β3 on mono and multilayer chondrocytes

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