Changes in the ratio of Bax and Bcl-2 mRNA expression and their cellular localization throughout the ovulatory cycle in the human oviduct

Briton-Jones, Christine; Lok, Ingrid Hung; Po, A.L.S.; Cheung, Che Kwok; Chiu, Tony Tak Yu; Haines, Christopher J.

doi:10.1007/s10815-005-9012-2

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…On the other hand, few apoptotic cells were detected during stages with high E 2 and low P 4 concentrations (early follicular phase) or stages with low levels of both E 2 and P 4 (anestrus, endometrial reparation). These findings are in general agreement with the occurrence of apoptotic processes in the oviduct of other mammalian species, such as cat, baboon, and human (Bareither and Verhage,1981; Verhage et al,1990; Briton‐Jones et al,2006). Similar observations have been published regarding apoptosis in the endometrium.…”

Section: Discussionsupporting

confidence: 86%

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Urhausen

Beineke

Marion

et al. 2010

The Anatomical Record

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Apoptotic cells were evaluated in the lamina epithelialis (LE) and lamina propria (LP) of the canine uterotubal junction and oviductal isthmus by means of the TUNEL assay and Caspase-3 detection regarding cyclic changes. Oviducts of bitches in anestrus, early and late follicular phase, 2 and 4 days after ovulation, mid-luteal phase, and endometrial reparation were available. The rate of apoptosis was generally low (min ¼ 0; max ¼ 33 apoptotic cells in 500 cells). Regardless of the stage of the estrous cycle, the number of apoptotic cells was significantly higher in the LE compared to the LP (P < 0.05). In anoestrus, early and late follicular phase as well as during endometrial reparation, the number of apoptotic cells was on a constantly low level. A significant increase was found 4 days after ovulation by caspase-3 detection coinciding with early luteal development and in mid-luteal phase. The results demonstrate that the number of apoptotic cells changes depending on the stage of the ovarian cycle. The coincidence of the increase in the number of apoptotic cells and the time of achievement of oocyte competence, which is known to occur in the bitch 3-4 days after ovulation, suggests a causal relationship between the postovulatory maturation stage of oocytes and the rate of apoptosis in the caudal oviduct. Furthermore, a specific relevance of apoptosis may exist in the process of fertilization regarding sperm storage and detachment of spermatozoa bound to the epithelium of the caudal oviduct, which has been identified as a functional sperm reservoir in other species. Anat Rec, 294:342-348, 2011. V V C 2010 Wiley-Liss, Inc.Key words: apoptosis; oviduct; uterotubal junction; isthmus; sperm reservoirThe oviduct plays an important role in reproduction by providing a suitable environment for oocyte pick up and maturation, sperm transport and storage, capacitation of spermatozoa, fertilization, and finally early embryonic development (Verhage et al., 1973a;Steinhauer et al., 2004;Rodriguez-Martinez, 2007). The canine estrous cycle differs considerably from that of other species, in that the follicular phase is prolonged and ovulation is followed by a prolonged luteal phase with an average duration of about 54-70 days in the nonpreg-*Correspondence to:

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Section: Discussionsupporting

confidence: 86%

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Urhausen

Beineke

Marion

et al. 2010

The Anatomical Record

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“…All data were presented as mean ± standard deviation (SD) and analyzed using IBM SPSS v. 19.0 (IBM Corp., Armonk, USA) and GraphPad Prism v. 7 (GraphPad Software, Bcl-2 Forward, 5'-TCCATGTCTTTGGACAACCA-3'; Reverse, 5'-CTCCACCAGTGTTCCCATCT-3' [27] Bax Forward, 5'-ATGGACGGGTCCGGGGAG-3'; Reverse, 5'-ATCCAGCCCAACAGCCGC-3' [28] Caspase-3 Forward, 5'-ATGGTTTGAGCCTGAGCAGA-3'; Reverse, 5'-GGCAGCATCATCCACACATAC-3' [29] GAPDH Forward, 5'-CAAGATCATCAGCAATGCCTCC-3'; Reverse, 5'-GCCATCACGCCACAGTTTCC-3' [30] U6 Forward, 5'-CTCGCTTCGGCAGCACATATAC-3'; Reverse, 5'-GGAACGCTTCACGAATTTGC-3' [30] San Diego, USA). Student's t-test (2 groups) and one-way analysis of variance (ANOVA) (3 or more groups, S-N-K method) were used to compare groups.…”

Section: Discussionmentioning

confidence: 99%

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway

et al. 2021

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Background. Increasing radiosensitivity of cancer cells can enhance the efficacy of cervical cancer treatment.Objectives. This study evaluated the potential roles and mechanism of baicalein in regulating the radiosensitivity of cervical cancer cells in vitro. Materials and methods.Real-time quantitative polymerase chain reaction (RT-qPCR) was used to assess miR-183 expression in End1/E6E7 cells, Hela cells and Hela cells irradiated with X-ray (0 Gy, 1 Gy, 3 Gy, 5 Gy, and 10 Gy). Cell Counting Kit-8 (CCK-8) method measured cell viability of Hela cells after miR-183 regulation, baicalein or RO8191 treatment. Apoptosis rates were detected using flow cytometry. Thereafter, expression of Bcl-2, Bax and caspase-3 RNA was also detected through RT-qPCR. Protein concentrations of E-cadherin, N-cadherin, Vimentin in epithelial-mesenchymal transition (EMT), phospho-JAK2/STAT3, and total Janus kinase 2/signal transducer and activator of transcription 3 STAT3 (JAK2/STAT3) were examined using enzyme-linked immunosorbent assay (ELISA) methods. RO8191, a JAK2/STAT3 activator, was used to activate the JAK2/STAT3 signaling pathway.Results. The miR-183 expression was significantly lower in Hela cells compared to End1/E6E7 cells. Following upregulation of miR-183 in Hela cells, cell viability was inhibited while apoptosis was promoted. Moreover, EMT was inhibited after miR-183 over-expression. X-ray treatment markedly reduced the cell survival rate and increased miR-183 RNA expression. Baicalein treatment severely reduced the cell viability of 10-Gy X-rayirradiated Hela cells, partially reversing the effect of miR-183, and also increased apoptosis and prevented EMT in irradiated cells. Y1007/8 in JAK2 and tyrosine (Tyr) residue 705 of STAT3 were phosphorylated, resulting in high expression of JAK2/STAT3, which was decreased by irradiation and baicalein treatment. RO8191 activated JAK2/STAT3 signaling, promoted cell viability and EMT, and inhibited cell apoptosis, while baicalein partly reversed the functions of RO8191.Conclusions. Baicalein inhibited cell viability and EMT, and induced cell apoptosis of Hela cells, through upregulating miR-183 via inactivation of the JAK2/STAT3 signaling pathway.

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“…Assessment of mRNA levels of brain Bcl-2/Bax and the housekeeping gene (β-actin) in brain tissue was performed using qPCR using Applied Biosystems step one plus equipment (Glare et al, 2002;Briton-Jones et al, 2006). Measurement of mRNA levels of peroxisome proliferator-activated receptor gamma (PPAR-γ), nuclear factor kappa B (NF-κB), Janus kinase-2 (JAK-2), signal transducers and activators of transcription (STAT-3), matrix metalloproteinase-2 (MMP-2), tissue inhibitor matrix metalloproteinase-1 (TIMP-1), alpha-smooth muscle actin (α-SMA), and the housekeeping gene (β-actin) in liver tissue was carried out by RT-qPCR using Applied Biosystems step one plus equipment.…”

Section: Real-time Quantitative Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

The protective effects of sesamol and/or the probiotic, Lactobacillus rhamnosus, against aluminum chloride-induced neurotoxicity and hepatotoxicity in rats: Modulation of Wnt/β-catenin/GSK-3β, JAK-2/STAT-3, PPAR-γ, inflammatory, and apoptotic pathways

et al. 2023

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Introduction: Aluminium (Al) is accumulated in the brain causing neurotoxicity and neurodegenerative disease like Alzheimer's disease (AD), multiple sclerosis, autism and epilepsy. Hence, attenuation of Al-induced neurotoxicity has become a “hot topic“ in looking for an intervention that slow down the progression of neurodegenerative diseases.Objective: Our study aims to introduce a new strategy for hampering aluminum chloride (AlCl3)-induced neurotoxicity using a combination of sesamol with the probiotic bacteria; Lactobacillus rhamnosus (L. rhamnosus) and also to test their possible ameliorative effects on AlCl3-induced hepatotoxicity.Methods: Sprague-Dawley male rats were randomly divided into five groups (n = 10/group) which are control, AlCl3, AlCl3 + Sesamol, AlCl3 + L. rhamnosus and AlCl3 + Sesamol + L. rhamnosus. We surveilled the behavioral, biochemical, and histopathological alterations centrally in the brain and peripherally in liver.Results: This work revealed that the combined therapy of sesamol and L. rhamnosus produced marked reduction in brain amyloid-β, p-tau, GSK-3β, inflammatory and apoptotic biomarkers, along with marked elevation in brain free β-catenin and Wnt3a, compared to AlCl3-intoxicated rats. Also, the combined therapy exerted pronounced reduction in hepatic expressions of JAK-2/STAT-3, inflammatory (TNF-α, IL-6, NF-κB), fibrotic (MMP-2, TIMP-1, α-SMA) and apoptotic markers, (caspase-3), together with marked elevation in hepatic PPAR-γ expression, compared to AlCl3 -intoxicated rats. Behavioral and histopathological assessments substantiated the efficiency of this combined regimen in halting the effect of neurotoxicity.Discussion: Probiotics can be used as an add-on therapy with sesamol ameliorate AlCl3 -mediated neurotoxicity and hepatotoxicity.

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Changes in the ratio of Bax and Bcl-2 mRNA expression and their cellular localization throughout the ovulatory cycle in the human oviduct

Cited by 8 publications

References 12 publications

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway

The protective effects of sesamol and/or the probiotic, Lactobacillus rhamnosus, against aluminum chloride-induced neurotoxicity and hepatotoxicity in rats: Modulation of Wnt/β-catenin/GSK-3β, JAK-2/STAT-3, PPAR-γ, inflammatory, and apoptotic pathways

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