Changes in type I collagen following laser welding

Bass, Lawrence S.; Moazami, Nader; Pocsidio, Joanne; Öz, Mehmet C.; LoGerfo, Paul; Treat, Michael R.

doi:10.1002/lsm.1900120508

Cited by 147 publications

(60 citation statements)

References 14 publications

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“…We found that crosslink content was afkcted by laser expomre, and that laser power output was a major determinant of laser effects on at least one crosslinlq HLNL. Our results diffix from those repted by Bass et al 3, who also looked at the effbct of diode laser+ KG exposwe on type I collagen. They infsrred fiorn their structural &ta that all covalent crosslinking is destroyed by laser exposure.…”

Section: Discussioncontrasting

confidence: 99%

Laser welding and collagen crosslinks

et al. 1997

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The strength and stabilityof laser-weldedtissue maybe influencedin pa@ by the effectsof laser exposure on collagen crosslinking.We thcmfm studiedthe effixts of diode laserexposure(805 nrn , 1-8 watts, 30 seconds) + indocyanine green dye (ICG) on calf tail tendon collagen crosslinks. 'Ihe efllsct of KG dye alone on crosslink content prior to laser exposure was investigated; unexpectedly, we found that KG-treated tissue had significantly increased DHLNL and OHP, but not HLNL. Laser exposure after 1(% application reduced elevated DHLNL and OHP crosslink content down to their native levels. The monobydrqdated crosslink HLNL was inversely cordated with laser output (pcO.O1 by linear regression analysis). DHLNL content was highly correlated with content of its maturational produce OHP, suggesting that precumr-pmduct relationships are maintained.We conclude that (1) ICG alone induces DHLNL and OHP crosslhk fonnatiom (2) subsequent laser exposure reduces the' ICG-induced crosslinks down to mtive levels (3) destroys normally occurring HLNL crosslii.

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Section: Discussioncontrasting

confidence: 99%

Laser welding and collagen crosslinks

et al. 1997

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“…Polom et al provide an excellent and further review of the utility of ICG-guided imaging for SLN detection, lymphedema evaluation, and assessment of microvascular circulation of free flaps in reconstructive surgery [21]. Additionally, ICG has been investigated for potential therapeutic applications such as photodynamic therapy (PDT) [22]- [24], tissue welding [25], and photothermal treatment of skin diseases [26], [27]. FOR in-vivo applications, ICG is administered by intravascular injection.…”

mentioning

confidence: 99%

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Jung

Vullev

Anvari

2014

IEEE J. Select. Topics Quantum Electron.

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Abstract-Indocyanine green (ICG) remains as the only near infrared dye approved by the FDA. Despite its long history of usage in clinical medicine, a systematic study of the effects of serum proteins at physiologically relevant levels and temperature on absorption and fluorescence characteristics of ICG has been missing. We incubated ICG at concentrations in the range of 0.6-25.8 μM in McCoy's 5a cell culture medium, without and with supplemental fetal bovine serum (FBS) at 5% and 10% levels. Our analyses of absorption and fluorescence spectra indicate that the peak absorbance of ICG associated with its monomeric form increases in the presence of FBS. For example, at ICG concentration of 25.8 μM, the monomer absorbance is increased by nearly 100% in the presence of 10% FBS. Similarly, there is an increase in the relative fluorescence quantum yield of ICG, by as much as nearly 3.5 times in the presence of FBS. When incubated at 37• C, the presence of FBS in the cell culture medium helps maintain the monomeric absorption of ICG and sustain the increased fluorescence emission. We offer explanations to describe the possible photophysical mechanisms underlying the observed effects and discuss the importance of these results to in-vivo applications of ICG.

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“…The increase in local temperature induces structural alterations in vessel wall proteins, which leads to protein cross-linking and consequent coaptation of the vessel segments. Although the exact types of structural alterations that result in welding are currently elusive [40,44,67], collagen cross-linking is considered to play a major role [4,33,40,44]. The extent to which these interprotein bonds are formed dictates the welding strength [4,33,40,44].…”

Section: Principal Mechanismmentioning

confidence: 99%

Laser-assisted vessel welding: state of the art and future outlook

Pabittei

Boon

Heger

et al. 2015

JCTRes

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Laser-assisted vascular welding (LAVW) is an experimental technique being developed as an alternative to suture anastomosis. In comparison to mechanical anastomosis, LAVW is less traumatic, non-immunogenic, provides immediate water tight sealant, and possibly a faster and easier procedure for minimally invasive surgery. This review focuses on technical advances to improve welding strength and to reduce thermal damage in LAVW. In terms of welding strength, LAVW has evolved from the photothermally-induced microvascular anastomosis, requiring stay sutures to support welding strength, to sutureless anastomoses of medium-sized vessels, withstanding physiological and supraphysiological pressure. Further improvements in anastomotic strength could be achieved by the use of chromophore-containing albumin solder and the employment of (biocompatible) polymeric scaffolds. The anastomotic strength and the stability of welds achieved with such a modality, referred to as scaffold-and solder-enhanced LAVW (ssLAVW), are dependent on the intermolecular bonding of solder molecules (cohesive bonding) and the bonding between solder and tissue collagen (adhesive bonding). Presently, the challenges of ssLAVW include (1) obtaining an optimal balance between cohesive and adhesive bonding and (2) minimizing thermal damage. The modulation of thermodynamics during welding, the application of semi-solid solder, and the use of a scaffold that supports both cohesive and adhesive strength are essential to improve welding strength and to limit thermal damage.

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Changes in type I collagen following laser welding

Cited by 147 publications

References 14 publications

Laser welding and collagen crosslinks

Laser welding and collagen crosslinks

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Laser-assisted vessel welding: state of the art and future outlook

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