Changes of heavy metal, metallothionein and heat shock proteins in Sertoli cells induced by cadmium exposure

Kusakabe, Takahiko; Nakajima, Katsuyuki; Nakazato, Kyoumi; Suzuki, Keiji; Takada, Hisashi; Satoh, Takahiro; Oikawa, Masakazu; Arakawa, Kazuo; Nagamine, Takahiko

doi:10.1016/j.tiv.2008.04.021

Cited by 53 publications

(29 citation statements)

References 61 publications

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“…The accumulation and toxic effects of cadmium have attracted considerable attention. Previous studies have shown that testis is the target organ of cadmium and that cadmium can damage testicular tissue and reduce spermatogenesis [34, 35]. Kusakabe et al , using ELISA, have reported that low concentration (2.5 µM) of cadmium doesn't induce DNA damage of rat Sertoli cells [35].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that testis is the target organ of cadmium and that cadmium can damage testicular tissue and reduce spermatogenesis [34, 35]. Kusakabe et al , using ELISA, have reported that low concentration (2.5 µM) of cadmium doesn't induce DNA damage of rat Sertoli cells [35]. We found, using the single-cell gel electrophoresis (comet assay), that cadmium chloride exposure with 1.2 mg produce significant elevations of tail moment in compare with control group as shown in Table 1and Figure 1, the affected nuclei of comet tail are also shown in (Figure 3, Figure 4).…”

Section: Discussionmentioning

confidence: 99%

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The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats

Selim¹,

Rashed²,

Aleisa³

et al. 2012

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Cadmium (Cd) is an environmental carcinogenic pollutant known to inactivate several proteins involved in DNA repair systems while at the same time creating an oxidative stress that can result in additional DNA lesions. The testis and the lung are the target organs for cadmium carcinogenesis. Increased production of oxidants in vivo can cause damage to intracellular macromolecules such as DNA, proteins and lipids, which in turn lead to oxidative injury. So, this investigation aimed to evaluate the protective role of L-Carnitine through up regulation of HSPs against DNA damage induced by cadmium chloride. The current study was carried out on forty adult male rats, each with average weight 220-250g., were divided into 4 equal groups. 1st group was received saline solution (0.5 ml/100 g body weight) and kept as control. 2nd group was received 500mg / kg body weight L-Carnitine intraperitoneally (IP). 3rd group was administered 1.2 mg cadmium chloride IP. 4th group was received both cadmium chloride and L-Carnitine simultaneously. The comet assay parameters showed significantly increased HSP70 and DNA damage in testis cells after 10 and 56 days in the third group. Meanwhile, HSP70 showed significantly decreased levels after 10 days and 56 days in the fourth group after L-Carnitine treatment simultaneously with cadmium chloride. The results of the present study demonstrate a damaging effect of cadmium chloride on DNA of the testis cells (with low stress response). This damaging effect increases the synthesis of HSP70 that upregulated by L-Carnitine treatment and showed ameliorative effect of the cells for recovery.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The protection role of heat shock protein 70 (HSP-70) in the testes of cadmium-exposed rats

Selim¹,

Rashed²,

Aleisa³

et al. 2012

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show abstract

“…The enzyme is induced during oxidative and inflammatory tissue injuries, including exposure to cadmium (Kusakabe et al, 2008). However, cadmium exposure causes marked increase in the generation of reactive oxygen species that exceeds the physiological capacity of the antioxidant enzymes and finally these enzymes are exhausted and their activity is reduced (Thijssen et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of hemin against cadmium-induced testicular damage in rats

et al. 2009

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“…Exposure to Cd may impair the capacity against oxidative injury by decreasing their own active oxygen and lipid peroxidation (Zhang et al, 2010). Changes of heavy metal, metallothionein and heat shock proteins in Sertoli cells were induced by Cd exposure (Kusakabe et al, 2008).…”

Section: Effect Of CD On Sertoli Supporting Cellsmentioning

confidence: 99%