Chapter 3 Biosynthesis of Terpenoid Indole Alkaloids in Catharanthus roseus Cells

Geraniol 10‐hydroxylase1, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

“…1). Strictosidine is the branch point from which biosynthesis routes diverge towards the various alkaloids found in di¡erent TIA-producing plant species [2,3]. Several studies using Catharanthus roseus indicate that the formation of secologanin can be a limiting factor in TIA production [4,5].…”

Cloning and Functional Analysis of Geraniol 10-Hydroxylase, a Cytochrome P450 fromSwertia mussotiiFranch

“…[5][6][7][8][9][10][11][12] CYP76B6 of C. roseus is significant in indole alkaloid synthesis. [5][6][7][8][9][10][11][12] Here, overexpression of SmG10H in transgenic plants promoted the accumulation of its downstream end-product swertiamarin (Fig. 9e, f).…”

“…1.14.14.1), a P450 in the CYP76B subfamily, hydroxylates geraniol at the C-10 position and is thought to be involved in the synthesis of indole alkaloids and iridoid monoterpenoid. [5][6][7][8][9][10][11][12] Similar G10H proteins have been extensively analyzed in several other species. [5][6][7][8][9] These studies have been focused on the importance of G10H in indole alkaloid synthesis, [8][9][10][11][12] but there is no longer a lack of research on iridoid monoterpenoid synthesis at the molecular level.…”

Role of vacuolar transporter proteins in plant secondary metabolism: Catharanthus roseus cell culture

“…1, Table 3), the regulation of the pathway has been difficult to characterize. The TIA biosynthesis pathway often involves multiple organellar compartments, resulting in transport limitation and sequestered pools of metabolites (Burlat et al 2004;Kutchan 2005;St-Pierre et al 1999;Verpoorte et al 1997). The early steps of the terpenoid pathway (leading to geraniol) and the tryptophan pathway are expected to occur in plastids (Poulsen and Verpoorte 1991;Zhang et al 2001).…”