Chapter 5 Methods and Protocols in Peripheral Nerve Regeneration Experimental Research

Raimondo, Stefania; Fornaro, Michele; Scipio, Federica Di; Ronchi, Giulia; Giacobini-Robecchi, Maria G.; Geuna, Stefano

doi:10.1016/s0074-7742(09)87005-0

Cited by 123 publications

(106 citation statements)

References 41 publications

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…Yet, if biomaterials are used to bridge nerve defects, histology can give important information also about degradation of the materials and the presence of foreign body reaction and granuloma. Many tissue processing and staining techniques -14 -are available and it is far beyond the goals of this paper to revise that body of literatue (see Raimondo et al, 2009;Carriel et al, 2014). Anyway, histology is adopted by the large majority of authors with the goal of quantifying the number and size of regenerated nerve fibers and myelin sheath thickness.…”

Section: Histology and Histomorphometrymentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

Self Cite

119

View full text Add to dashboard Cite

In the pre-clinical view, the study of peripheral nerve repair and regeneration still needs to be carried out in animal models due to the structural complexity of this organ which can be only partly simulated in vitro. The far most used experimental model is based on the injury of the sciatic nerve, the largest nerve trunk in mammals. In this paper, the potential application of the sciatic nerve injury model in pre-clinical research is critically reviewed. This paper is aimed at helping researchers in properly employing this in vivo model for the study of nerve repair and regeneration as well as interpreting the results in a clinical translation perspective.

show abstract

Section: Histology and Histomorphometrymentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

Self Cite

119

View full text Add to dashboard Cite

show abstract

“…For histomorphometric analysis, nerve samples obtained from the 10-mm-long sciatic nerve segments distal to the neurotmesis site and from un-operated controls [41][42][43][44] . The histological nerve preparation followed the previously reported protocol [3,9,17,25,31,33,35] and a systematic random sampling and D-disector were adopted [41][42][43][44] .…”

Section: Morphological Analysis and Histopathologymentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

View full text Add to dashboard Cite

“…The proximal end was marked with 9-0 Nylon. After paraffin inclusion (Raimondo et al, 2009), transversal sections (11μm thickness) were obtained and stained with Sirius Red following previously described protocol (Carriel et al 2011).…”

Section: Introductionmentioning

confidence: 99%