Characterisation of blood microbiota in patients with liver cirrhosis

Kajihara, Mikio; Koido, Shigeo; Kanai, Tomoya; Ito, Zensho; Matsumoto, Yoshihiro; Takakura, Kazuki; Saruta, Masayuki; Kato, Koichi; Odamaki, Toshitaka; Xiao, Jin‐zhong; Sato, Nobuhiro; Ohkusa, Toshifumi

doi:10.1097/meg.0000000000001494

Cited by 39 publications

(41 citation statements)

References 20 publications

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“…Analysis of gut microorganisms revealed significant differences between CVT and SPF mice showing effective transfer of microorganisms from CVB to CVT mice through co-housing. The top three OTUs highly represented in CVB mice and effectively transferred to CVT mice during co-housing were Prevotellaceae, Lactobacillaceae and Rikenellaceae , which is consistent with our previous observation in CByB6F1 mice born and housed in the same conventional facility in which the same three microbial families were most abundant in CVB mice [ 17 ].These microbial families offer health benefits, since lack of Prevotellaceae and Rikenellaceae have been associated with Parkinson’s disease [ 21 ], and liver cirrhosis [ 22 ]. In Rag1-/- mice, fecal transplants from wild type donors altered gut bacterial community with increases in Prevotellaceae which were accompanied by increases in total number of BM cells and HSPCs [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression

Chen

Zhang

Feng

et al. 2020

IJMS

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Specific-pathogen-free (SPF) mice have improved hematopoietic characteristics relative to germ-free mice, however, it is not clear whether improvements in hematopoietic traits will continue when the level of microorganism exposure is further increased. We co-housed SPF C57BL/6 mice in a conventional facility (CVT) and found a significant increase in gut microbiota diversity along with increased levels of myeloid cells and T cells, especially effector memory T cells. Through single cell RNA sequencing of sorted KL (c-Kit+Lin−) cells, we imputed a decline in long-term hematopoietic stem cells and an increase in granulocyte-monocyte progenitors in CVT mice with up-regulation of genes associated with cell survival. Bone marrow transplantation through competitive repopulation revealed a significant increase in KSL (c-Kit+Sca-1+Lin−) cell reconstitution in recipients of CVT donor cells which occurred when donors were co-housed for both one and twelve months. However, there was minimal to no gain in mature blood cell engraftment in recipients of CVT donor cells relative to those receiving SPF donor cells. We conclude that co-housing SPF mice with mice born in a conventional facility increased gut microbiota diversity, augmented myeloid cell production and T cell activation, stimulated KSL cell reconstitution, and altered hematopoietic gene expression.

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Section: Discussionmentioning

confidence: 99%

Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression

Chen

Zhang

Feng

et al. 2020

IJMS

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“…The cirrhosis dysbiosis ratio may be a useful quantitative indicator to describe the changes in the microbiome accompanying the progression of cirrhosis. Interestingly, a recent study has found that the diversity of circulating bacteria in patients with cirrhosis is consistent with the presence of dysbiosis in cirrhotics[ 155 ]. Moreover, specific taxa of gut microbes are associated with changes in neurons and astrocytes in brain dysfunction associated with cirrhosis[ 156 ].…”

Section: Microbiota-gut-live-brain Axis and Cirrhosis And Hementioning

confidence: 97%

Role of gut microbiota via the gut-liver-brain axis in digestive diseases

Ding¹,

Jin²,

Yang³

et al. 2020

WJG

112

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The gut-brain axis is a bidirectional information interaction system between the central nervous system (CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex network with the enteric nervous system, the autonomic nervous system, and the neuroendocrine and neuroimmunity of the CNS, which is called the microbiota-gut-brain axis. Due to the close anatomical and functional interaction of the gut-liver axis, the microbiota-gut-liver-brain axis has attracted increased attention in recent years. The microbiota-gut-liver-brain axis mediates the occurrence and development of many diseases, and it offers a direction for the research of disease treatment. In this review, we mainly discuss the role of the gut microbiota in the irritable bowel syndrome, inflammatory bowel disease, functional dyspepsia, non-alcoholic fatty liver disease, alcoholic liver disease, cirrhosis and hepatic encephalopathy via the gut-liver-brain axis, and the focus is to clarify the potential mechanisms and treatment of digestive diseases based on the further understanding of the microbiota-gut- liver-brain axis.

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“…Kajihara and coworkers examined the peripheral blood microbiota of patients with liver cirrhosis by 16S rRNA gene sequencing and found that while the abundance of Enterobacteriaceae was significantly increased, the abundance of Akkermansia, Rikenellaceae and Erysipelotrichales was decreased in cirrhotic patients (27).…”

Section: Non-intestinal Microbiotas and Liver Diseasesmentioning

confidence: 99%

“…Figure 2 Summary of studies on non-intestinal microbiotas in diverse liver diseases (24)(25)(26)(27)(28)(29)(30)(31). HCC, hepatocellular carcinoma.…”

Section: Biogeography Of the Liver Tissue Bacterial Dna Of Patients With Nafldmentioning

confidence: 99%

Liver tissue microbiota in nonalcoholic liver disease: a change in the paradigm of host-bacterial interactions

Sookoian¹,

Pirola²

2021

Hepatobiliary Surg Nutr

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Nonalcoholic fatty liver disease (NAFLD) pathogenesis is explained by the complex relationship among diet and lifestyle-predisposing factors, the genetic variance of the nuclear and mitochondrial genome, associated phenotypic traits, and the yet not fully explored interactions with epigenetic and other environmental factors, including the microbiome. Despite the wealth of knowledge gained from molecular and genome-wide investigations in patients with NAFLD, the precise mechanisms that explain the variability of the histological phenotypes are not fully understood. Earlier studies of the gut microbiota in patients with NAFLD and nonalcoholic steatohepatitis (NASH) provided clues on the role of the fecal microbiome in the disease pathogenesis. Nevertheless, the composition of the gut microbiota does not fully explain tissuespecific mechanisms associated with the degree of disease severity, including liver inflammation, ballooning of hepatocytes, and fibrosis. The liver acts as a key filtration system of the whole body by receiving blood from the hepatic artery and the portal vein. Therefore, not only microbes would become entrapped in the complex liver anatomy but, more importantly, bacterial derived products that are likely to be potentially powerful stimuli for initiating the inflammatory response. Hence, the study of liver tissue microbiota offers the opportunity of changing the paradigm of host-NAFLD-microbial interactions from a "gut-centric" to a "liver-centric" approach. Here, we highlight the evidence on the role of liver tissue bacterial DNA in the biology of NAFLD and NASH. Besides, we provide evidence of metagenomic findings that can serve as the seed of further hypothesis-raising studies as well as can be leveraged to discover novel therapeutic targets.

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Characterisation of blood microbiota in patients with liver cirrhosis

Abstract: Supplemental Digital Content is available in the text.

Cited by 39 publications

References 20 publications

Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression

Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene Expression

Role of gut microbiota via the gut-liver-brain axis in digestive diseases

Liver tissue microbiota in nonalcoholic liver disease: a change in the paradigm of host-bacterial interactions

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