Characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008–2012

Tatte, Vaishali S.; Chothe, Nital S.; Chitambar, Shobha D.

doi:10.1016/j.vaccine.2014.03.009

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…Previous studies have described RVA in adults pre‐ and post massive introduction of RVA vaccination [Nakajima et al, ; Morillo et al, ; Gunn et al, ; Anandan et al, ; Kirkwood and Roczo‐Farkas, ; Kittigul et al, ; Luchs et al, ; Tatte et al, ; Anderson et al, ; Pacilli et al, ]. In our data, G12P[8] was observed in high prevalence in adults, in accordance with previous reports reinforcing the idea that adults may have more RVA infections than previously reported.…”

Section: Resultssupporting

confidence: 92%

VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Silva

Fumian

Assis

et al. 2016

Journal of Medical Virology

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Group A rotavirus (RVA) genotype G12 is habitually associated with diarrhea disease (DD) in African children and recently its detection has increased worldwide. A total of 970 stool samples collected from individuals with DD in the Northeastern, Southeastern, and Southern Brazilian regions, Eastern coast, were analyzed and 321 (33%) were positive for RVA and of these, 241 (75%) genotyped as G12P[8]. The rate of RVA positivity was higher among children aged 5-10 years old (60%). All RVA infections observed in adults aged >21 years were G12P[8] (n = 27) showing that this genotype affected older age groups during the year of 2014 in Brazil. Phylogenetic analysis of VP7 and VP8* G12P[8] strains demonstrated an elevated similarity among Brazilian and G12-III prototypes strains circulating worldwide recently, suggesting that this lineage is associated with the global spread of the G12 genotype, considered as the 6th most prevalent human RVA genotype nowadays; while other G12 lineages remain sporadically detected and usually detected in association with other P genotypes. VP8* analysis revealed that Brazilian strains belong to P[8]-3 lineage, the single P[8] lineage presently detected in the country. No major nucleotide/amino acid disparities were observed among strains recovered from children and adults for VP7 and VP8* genes. These data are essential to support the surveillance studies, particularly in countries where the RVA vaccine was introduced in their National Immunization Program enabling identification of potential alterations in the epidemiological profile that can impact its efficacy in vaccination programs. J. Med. Virol. 89:64-70, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Resultssupporting

confidence: 92%

VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Silva

Fumian

Assis

et al. 2016

Journal of Medical Virology

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“…The significant proportion of partially typed samples, which we found in the control group, may be explained by the fact that controls may excrete less virus than children with more severe diarrhoea, as observed by other groups [51]. In addition to this, few available studies that investigated rotavirus on LSD cases have reported a high proportion of non-typeable strains [52,53]. A study in India reported 80% and another in China 24% [52,53].…”

Section: Discussionmentioning

confidence: 58%

Molecular Epidemiology of Rotavirus Strains in Symptomatic and Asymptomatic Children in Manhiça District, Southern Mozambique 2008–2019

Manjate

João

Chirinda

et al. 2022

Viruses

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Group A rotaviruses remain the leading cause of diarrhoea in children aged <5 years. Mozambique introduced rotavirus vaccine (Rotarix®) in September 2015. We report rotavirus genotypes circulating among symptomatic and asymptomatic children in Manhiça District, Mozambique, pre- and post-vaccine introduction. Stool was collected from enrolled children and screened for rotavirus by enzyme-immuno-sorbent assay. Positive specimens were genotyped for VP7 (G genotypes) and VP4 (P genotypes) by the conventional reverse transcriptase polymerase chain reaction. The combination G12P[8] was more frequently observed in pre-vaccine than in post-vaccine introduction, in moderate to severe diarrhoea (34%, 61/177 vs. 0, p < 0.0001) and controls (23%, 26/113 vs. 0, p = 0.0013) and mixed genotypes (36%, 24/67 vs. 7% 4/58, p = 0.0003) in less severe diarrhoea. We observed changes in post-vaccine compared to pre-vaccine introduction, where G3P[4] and G3P[8] were prevalent in moderate to severe diarrhoea (10%, 5/49 vs. 0, p = 0.0002; and 14%, 7/49 vs. 1%, 1/177, p < 0.0001; respectively), and in less severe diarrhoea (21%, 12/58 vs. 0, p = 0.003; and 24%, 14/58 vs. 0, p < 0.0001; respectively). Our surveillance demonstrated the circulation of similar genotypes contemporaneously among cases and controls, as well as switching from pre- to post-vaccine introduction. Continuous surveillance is needed to evaluate the dynamics of the changes in genotypes following vaccine introduction.

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“…In Latin America, the prevalence of G9P[4], which had been found in 0.4% of RVA cases, remarkably increased in 2009–2010 [Quaye et al, ]. A recent study reported that a G9P[4] strain having I2‐type VP6 and E6‐type NSP4, which is identical VP7‐VP4‐VP6‐NSP4 set with G9P[4] strain in Latin America, was detected in India during 2010–2011, suggesting that a certain G9P[4] strain may be circulating globally [Quaye et al, ; Tatte et al, ]. The phylogenetic analysis of the G9P[4] strain in Japan, which includes its unique genome constellation possessing genotype 2 internal protein genes except for the T1‐type NSP3 gene, revealed that this strain is distinct from other reported G9P[4] strains but closely related to co‐circulating Japanese G9P[8] strain in VP7 and NSP3 genes.…”

Section: Discussionmentioning

confidence: 99%

“…Putative binding domains (BD) in NSP3 with RNA, RoXaN, and eIF4G were indicated as RNA-BD, RoXaN-BD, and eIF4G-BD, respectively [Arnold et al, 2012]. strain in Latin America, was detected in India during 2010-2011, suggesting that a certain G9P[4] strain may be circulating globally [Quaye et al, 2013;Tatte et al, 2014]. The phylogenetic analysis of the G9P[4] strain in Japan, which includes its unique genome constellation possessing genotype 2 internal protein genes except for the T1-type NSP3 gene, revealed that this strain is distinct from other reported G9P[4] strains but closely related to co-circulating Japanese G9P[8] strain in VP7 and NSP3 genes.…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of a human G9P[4] rotavirus strain in Japan

Yamamoto

Kaida

Ono³

et al. 2015

Journal of Medical Virology

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In a surveillance system in Osaka City, Japan, 48 sporadic rotavirus A (RVA) infections were detected during 2008/2009-2011/2012 seasons. The G/P-genotypes of detected RVAs were G1P[8], G2P[4], G3P[8], G9P[4], and G9P[8]. Although G9P[4] is a rare genotype that had not been reported in Japan, it was the second most prevalent genotype, following G1P[8], and accounted for 35.3% of RVA cases in the 2011/2012 season. Further genotyping revealed that the G9P[4] strain had genotype 2 internal protein genes except for NSP3: G9-P[4]-I2-R2-C2-M2-A2-N2-T1-E2-H2. Among detected RVA strains, G9P[4] and some G9P[8] strains shared high nucleotide identity in VP7 and NSP3 genes. Phylogenetic and BLAST search analyses showed that the G9P[4] strain in Japan shared high nucleotide identity in genotype 2 genes with common G2P[4] strains circulating globally, but was distinct from other G9P[4] strains circulating worldwide. These results suggest that the G9P[4] strain in Japan may have emerged through an independent reassortment between G9P[8] and G2P[4]. Finally, the role of NSP3 protein in the circulating RVA from an amino acid comparison between T1- and T2-type NSP3 is discussed. These findings provide an important insight into less problematic combinations of circulating RVA genes derived from different genotypes.

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Characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008–2012

Cited by 11 publications

References 28 publications

VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Molecular Epidemiology of Rotavirus Strains in Symptomatic and Asymptomatic Children in Manhiça District, Southern Mozambique 2008–2019

Detection and characterization of a human G9P[4] rotavirus strain in Japan

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