Characterisation of the external surfaces of Pseudomonas aeruginosa isolated from human blood and respiratory tract

Abstract

Summary. The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freezesubstitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous layer c. 30 nm thick was found around the outer-membrane surface, whereas no such structure was observed in the other group of isolates, most of which were from sputum. Lipopolysaccharides extracted from the isolates with a dense fibrous layer were found by SDS-PAGE to have long 0-polysaccharide chains, whereas strains without such a layer mostly had lipopolysaccharides that lacked high mol. wt. 0-polysaccharide chains.

Heterogeneity in Expression of Lipopolysaccharide and Major Outer‐Membrane Proteins by Strains of Escherichia coli O157 with Different H‐Serotypyes

“…2D). Since these data are consistent with those of LPS ladder patterns in SDS-PAGE and the structure is very similar to the LPSs of Pseudomonas aeruginosa (13) and Klebsiella pneumoniae (11), the layers detected in this study were probably the polysaccharide region of the LPS.…”

Surface Characteristics of Gram‐Negative and Gram‐Positive Bacteria in an Atomic Force Microscope Image

“…The clinical strains of P aeruginosa are known to have two lipopolysaccharide (LPS) types (8). The strains isolated from patients with respiratory infections are of Fig.…”