Characteristics and readout correlation of flow-injection analysis for penicillin

Peniclllinase and penicillin amidase were immobilized, with similar yield, by physical entrapment in porous beads of poly-2-hydroxyethyl methacrylate matrix formed by gamma-irradiation. Flow-injection analysis of penicillins was performed by using a column reactor filled with enzyme-loaded polymer beads and a wall-jet pH-metric micro-cell with a combined flat-membrane glass electrode as the sensing element. With both enzymes it was possible to have either a linear response dynamic range or a logarithmic one by appropriate selection of buffer capacity and flow rate of the carrier solution.

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Characteristics and readout correlation of flow-injection analysis for penicillin

Cited by 3 publications

References 12 publications

On-line solid-phase reactors for unsegmented continuous-flow drug analysis

On-line solid-phase reactors for unsegmented continuous-flow drug analysis

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Potentiometric Biosensing of Penicillins Using a Flow-Through Reactor with Penicillinase or Penicillin Amidase Immobilized by Gamma-Irradiation

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