Characteristics of ciprofloxacin resistant Campylobacter spp. isolated from chicken meat in Turkey

Kürekçi, Cemil; Önen, Sevda Pehlivanlar

doi:10.1007/s11694-016-9426-9

Cited by 4 publications

(5 citation statements)

References 37 publications

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“…In this study, all ciprofloxacin resistant Campylobacters have Thr86Ile substitution in the QRDR of gyrA. Recently, Kurekci and Pehlivanlar Önen [33] have also identified the gyrA mutation of Thr86Ile among all Campylobacter spp. isolated from chicken meat sold in Turkey.…”

Section: Aslantaşmentioning

confidence: 56%

“…isolated from chicken meat sold in Turkey. Authors also identified Ala40Ser mutation in a C. jejuni strain [33] .…”

Section: Aslantaşmentioning

confidence: 97%

“…Resistance to ampicillin and other β-lactams have widely been reported among Campylobacter isolates from humans, poultry and food of animal origins [5,7,22,33] . A novel class D β-lactamase gene, bla OXA-61 , described by Alfredson and Korolik [34] has been held responsible for resistance to the β-lactams including ampicillin, piperacillin, and carbenicillin.…”

Section: Aslantaşmentioning

confidence: 99%

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Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Aslantaş¹

2017

Kafkas Univ Vet Fak Derg

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The objective of this study was to investigate the prevalence, antimicrobial resistance and genetic determinants of resistance of Campylobacter jejuni and Campylobacter coli isolated from commercial broiler farms in Adana and Hatay provinces, Turkey. The assessment of the genetic diversity among the isolates was determined by flaA based RFLP-Polymerase Chain Reaction (PCR) with restriction enzyme DdeI and sequence analysis of short variable regions (SVRs) of flaA and flaB-SVR genes. Antimicrobial susceptibility of the isolates was performed by disk diffusion method and tetracycline (tetO), ampicillin (blaOXA-61), aminoglycoside (aph-3-1) and multidrug efflux pump (cmeB) resistance genes were investigated by PCR as well. The genes conferring resistance to ciprofloxacin was screened by PCR and following DNA sequencing. The presence of ten virulence (flaA, virB11, racR, cadF, ciaB, dnaJ and pldA) and toxin genes (cdtA, dtB, cdtC) among the isolates was also investigated using PCR. Out of 220 cloacal swabs, 218 (99.1%) Campylobacter spp. including C. jejuni (n=194; 89%) and C. coli (n=24; 11%) were isolated. While all the isolates were susceptible to chloramphenicol, gentamicin and erythromycin, resistance rates for C. jejuni and C. coli isolates to nalidixic acid, ciprofloxacin, ampicillin, tetracycline and amoxicillin-clavulanic acid were determined as 86.6-100%, 86.6-100%, 45.9-45.8%, 43.3-50% and 2.6-0.0%, respectively. The tetO, blaOXA-61, cmeB and aph-3-1 genes detected in C. jejuni and C. coli isolates were 45.3-54.2%, 36.1-75%, 1.5-83.3% and 0.5-0%, respectively.

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Section: Aslantaşmentioning

confidence: 56%

“…isolated from chicken meat sold in Turkey. Authors also identified Ala40Ser mutation in a C. jejuni strain [33] .…”

Section: Aslantaşmentioning

confidence: 97%

Section: Aslantaşmentioning

confidence: 99%

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Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Aslantaş¹

2017

Kafkas Univ Vet Fak Derg

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“…Determination of antimicrobial resistance genes . The isolates phenotypically determined as resistant were examined by PCR in the broad sense of antimicrobial resistance (3, 15, 22, 27, 34, 35). A total of 25 μL of PCR mixture contained 2.5 μL of 10× PCR buffer, 1.5 μL of M gCl2 (25 mM), 1.25 μL of dNTP (2 mM), 0.25 μL of each primer (1.0 mg/mL), 0.2 μL of Taq polymerase (5 U/μL, Takara Bio Inc, Japan), and 1 μL of target DNA.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey

Adıgüzel

Sığırcı

Çelik

et al. 2018

Journal of Veterinary Research

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IntroductionThe study aimed to isolate thermophilic Campylobacter from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.Material and MethodsCloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79). The samples were cultured on modified charcoal cefoperazone desoxycholate (mCCD) agar. Suspect isolates were identified with multiplex PCR (mPCR). As per EUCAST standards, MIC values were derived by broth microdilution for tetracycline, ciprofloxacin, nalidixic acid, kanamycin, gentamicin, and erythromycin in isolates of C. jejuni (n = 98) and C. coli (n = 83).ResultsIn C. jejuni, 78.6% tetracycline, 87.8% ciprofloxacin, and 81.6% nalidixic acid resistance was detected, but none was to kanamycin, gentamicin, or erythromycin. In C. coli, 98.8% ciprofloxacin and 63.9% nalidixic acid resistance was detected, whereas resistance to non-quinolones was not observed. C257T (Thr-86-Ile) mutation in the gyrA gene of all phenotypically quinolone-resistant isolates was detected through a mismatch amplification mutation assay PCR (MAMA-PCR). It emerged that all isolates bore the tet (O) resistance gene.ConclusionCommon tetracycline, nalidixic acid, and ciprofloxacin resistance exists in Campylobacter isolated from chickens raised three rearing methods.

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“…According to our findings, the rate of ciprofloxacin resistance (99.2%) in Campylobacter isolates is quite high. The fact that the same antimicrobials are frequently used as first-line treatments in broiler flocks to combat bacterial illnesses can be blamed for the high rate of resistance to ciprofloxacin, which is equivalent to that found in Italy [12][13][14]. Several studies have unequivocally demonstrated a link between the use of fuoroquinolones in chicken production and a rise in the resistance of Campylobacter isolates of avian and human origins [15].…”

Section: Discussionmentioning

confidence: 99%

First Report of aac(6′)-Ib and aac(6′)-Ib-cr Variant Genes Associated with Mutations in gyrA Encoded Fluoroquinolone Resistance in Avian Campylobacter coli Strains Collected in Tunisia

Gharbi,

Abbas,

Hamrouni

et al. 2023

IJMS

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The aac(6′)-Ib gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant aac(6′)-Ib-cr gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 Campylobacter isolates, including 91 C. jejuni and 41 C. coli, were selected from broiler hens isolates. The aac(6′)-Ib gene was amplified using PCR and was subsequently digested with the BtsCI restriction enzyme to identify aac(6′)-Ib-cr. Among these isolates, 31 out of 41 C. coli (75.6%) and 1 (0.98%) C. jejuni were positive for the aac(6′)-Ib gene, which was identified as the aac(6′)-Ib-cr variant in 10 (32.25%) C. coli isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on Campylobacter coli strains harboring both the aac(6′)-Ib and aac(6′)-Ib-cr variants. These genes were present in Campylobacter isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments.

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Characteristics of ciprofloxacin resistant Campylobacter spp. isolated from chicken meat in Turkey

Cited by 4 publications

References 37 publications

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey

First Report of aac(6′)-Ib and aac(6′)-Ib-cr Variant Genes Associated with Mutations in gyrA Encoded Fluoroquinolone Resistance in Avian Campylobacter coli Strains Collected in Tunisia

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