Characteristics of cow's milk proteins including allergenic properties and methods for its reduction

Miciński, Jan; Kowalski, Ireneusz M.; Zwierzchowski, Grzegorz; Szarek, J.; Pierożyński, Bogusław; Zabłocka, Elwira

doi:10.1016/j.poamed.2013.07.006

Cited by 39 publications

(35 citation statements)

References 26 publications

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“…β‐Casein can be cleaved by the milk plasmin (native protease) originating another family of caseins (γ 1‐, γ 2‐, and γ 3 ), although they are considered to be nonallergenic (Kumosinski and others ; Wal ; Chatchatee and others ; Micínski and others ; Goff ). Regarding the identification of IgE‐binding epitopes on β‐casein, Otani and others () concluded that there are at least 6 antigenic sites on the molecule and that the epitopes are sequential (Wal ).…”

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

“…The CMP has 64 aa and it is responsible for the reduction of gastric acid and serum gastrin secretion, increasing the efficiency of digestion. It also exhibits anticoagulant properties, prevents platelet agglomeration, and serotonin secretion (Micínski and others ; Goff ). The number of O‐glycosylation sites in CMP can vary from 0 to 7, so both nonglycosylated and glycosylated isoforms exists in digested milk.…”

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

“…Food allergies associated with this protein are estimated in 80% of the population (Micínski and others ). For this reason, several studies focusing on the identification of IgE‐binding regions have been performed.…”

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

“…Besides its function as a scavenger of free radicals and as an antioxidant, its main role is to defend the organism against infections and inflammations owing to its ability to sequester iron from the environment and, thereby, removing this essential nutrient for bacterial growth (Ward and Conneely ). In addition, it is involved in detoxification processes and it has an antineoplastic effect by inhibiting the attachment of tumor growth factors (Micínski and others ). Some studies declare that some milk‐allergic individuals possess LF‐specific IgE, although the relevance of the allergenicity of this protein is still under discussion because these patients also present IgE against one of the major milk allergens.…”

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

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Bovine Milk Allergens: A Comprehensive Review

Villa

Costa

Oliveira

et al. 2017

Comp Rev Food Sci Food Safe

190

145

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Cow milk allergy is one of the most common food allergies in early childhood and often persists through adult life, forcing an individual to a complete elimination diet. Milk proteins are present in uncounted food products, such as cheese, yogurt, or bakery item, exposing allergic persons to a constant threat. Many efforts have been made to overcome this global problem and to improve the life quality of allergic individuals. First, proper and reliable food labeling is fundamental for consumers, but the verification of its compliance is also needed, which should rely on accurate and sensitive analytical methods to detect milk allergens in processed foods. At the same time, strategies to reduce milk allergenicity, such as immunotherapy or the use of food processing techniques to modify allergen structure, have to be extensively studied. Recent research findings on the applicability of food processing, such as heat treatment, fermentation, or high pressure, have revealed great potential in reducing milk allergenicity. In this review, significant research advances on cow milk allergy are explored, focusing on prevalence, diagnosis, and therapy. Molecular characterization of cow milk allergens and cross-reactivity with other nonbovine milk species are described, as well as the effects of processing, food matrix, and digestibility on milk allergenicity. Additionally, analytical methods for the detection of milk allergens in food are described, from immunoassays and mass spectrometry methods for protein analysis to real-time polymerase chain reaction for DNA analysis.

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Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

Section: Molecular Characterization Of Milk Allergensmentioning

confidence: 99%

See 2 more Smart Citations

Bovine Milk Allergens: A Comprehensive Review

Villa

Costa

Oliveira

et al. 2017

Comp Rev Food Sci Food Safe

190

145

View full text Add to dashboard Cite

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“…Actualmente, la producción total del país se estima en 5,5 millones de litros de leche por día, con el 73 % proveniente de la región central del país (INEC, 2014) y su principal destino es el abastecimiento del mercado interno. La leche, por su composición nutricional, destaca como un alimento completo que contiene proteínas, vitaminas, lípidos y minerales con beneficios biológicos importantes en la etapa de crecimiento y en el mantenimiento óseo del cuerpo (Micinski, 2013;Pereira, 2014). La globalización se presenta como el principal responsable para el aumento del consumo de leche y derivados, influenciado por cambios en los patrones alimenticios, el crecimiento poblacional y la prioridad de la sociedad actual, de disponer de alimentos nutritivos y sanos (Primary Industries, 2015;CEPAL, 2017).…”

Section: Introductionunclassified

Estudio en el Ecuador de la curva de calibración para el conteo total de bacterias por citometría de flujo de leche cruda bovina

Contero

Aquino

Simbaña

et al. 2019

lgr

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ResumenEl análisis del conteo total de bacterias (CBT) como parámetro de la calidad higiénica en leche cruda es uno de los más requeridos mundialmente por las industrias y entidades de vigilancia de la inocuidad de alimentos. Este análisis se realiza tradicionalmente por el método referencial del conteo en placas expresado en UFC/mL, sin embargo, el método alternativo por citometría de flujo actualmente, se muestra como un método confiable y más rápido, que permite determinar las poblaciones bacterianas a través del Conteo Individual de Bacterias (IBC/ml). El objetivo del presente estudio fue determinar la ecuación de regresión y correlación entre ambos métodos debido a que éstos pueden variar en cada país en función de las actividades de manejo del ganado durante el ordeño. Para su evaluación en Ecuador, fueron utilizadas 357 muestras de leche con rangos entre 100000 a 1, 5 millones de UFC/mL provenientes de dos pisos altitudinales: Zona 1 (Z1) (> 3000 msnm) y Zona 2 (Z2) (1050 msnm). Los análisis fueron realizados bajo ambos métodos simultáneamente, (máximo de desviación de ± 1 h) y siguiendo las recomendaciones de la IDF/ISO-196 y la norma AOAC 986,33. Los resultados mostraron una alta correlación entre los métodos (r = 0, 91) y al no encontrar diferencias estadísticas significativas para el efecto de las zonas (Z1 y Z2), el mismo no fue considerado, sugiriendo el uso de una única ecuación de regresión lineal. Palabras clave: CBT, IBC, Bactoscan, citometría, calidad de leche. Abstract 97The total bacteria count (TBC) analysis, as a parameter of hygienic quality in raw milk, is one of the most requested analysis by dairy industries and food safety organizations. This analysis is done by the reference method of plate count expressed as colony-forming unit per milliliter (CFU/mL). However, the alternative method by flow cytometry currently is a reliable and faster method than the reference method of Individual Bacteria Count per milliliter (IBC/mL). The aim of the study was to determine the linear regression equation and correlation between the reference method and the alternative method by flow cytometry. Because the activities of livestock management during milking are different in each country, a total of 357 milk samples with a range from 100000 to 1,5 millions of UFC/mL from two altitudinal floors: Z1 (> 3000 masl) and Z2 (1050 masl) were done in Ecuador. Both methods were analyzed at the same time, (maximum deviation of ± 1 h), following the recommendations of the IDF/ISO − 196 and the AOAC 986.33 standard. The results showed a high correlation between the two methods (r = 0,91) and since there were no statistic differences for the effect of Zones 1 and 2, it was not considered, suggesting the use of a unique linear regression equation.

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