Quantification of soil is crucial for maximizing the advantages of land resources while minimizing the negative consequences of land degradation in the long term. It will also make it possible to identify locations that need immediate soil erosion management. The present study was carried out in the Kulfo river catchment, Rift valley, Southern Ethiopia. The Revised Universal Soil Loss Equation (RUSLE) method was utilized to estimate the mean yearly soil loss in the research region using remote sensing, other collateral data. The RUSLE model inputs were mapped and integrated into the ArcGIS software, and the results show that 0 and 1211 t ha−1year−1 are the minima and maximum soil loss in the present study area. Soil erosion-prone regions were divided into three categories: 0-42 t ha−1year−1 (low), 43-128 t ha−1 year−1 (medium), and > 128 t ha−1 year−1 (high). And the average rate of soil erosion is 68.47 t ha−1year−1. Low, medium, and high soil erosion areal extent and area percentages in the current research area is 270 km2 (77 %), 61 km2 (17 %), and 19 km2 (6%), respectively. A high rate of soil erosion was found where high steep slope, barren land, and high precipitation occurred in the present study area. The current study's outcomes were confirmed by comparing soil loss estimates in the same geo-environmental conditions found in Ethiopia's highlands. The outcome of this study is important for decision-makers and policymakers.