Characteristics of
            <i>Streptococcus pyogenes</i>
            Serotype M1 and M3 Isolates from Patients in Japan from 1981 to 1997

Murase, Toshiyuki; Suzuki, Riéko; Osawa, Ro; Yamai, Shiro

doi:10.1128/jcm.37.12.4131-4134.1999

Cited by 17 publications

(7 citation statements)

References 18 publications

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“…When we analyzed the GAS isolates used in this study by PFGE after SmaI digestion, we found that their PFGE patterns agreed with those reported previously (Stanley et al , 1995; Murase et al , 1999) (Fig. 1).…”

Section: Resultssupporting

confidence: 89%

Positive correlation between low adhesion of group AStreptococcusto mammalian cells and virulence in a mouse model

Miyoshi‐Akiyama

Zhao

Uchiyama

et al. 2009

FEMS Microbiology Letters

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We previously reported that a mouse model reflected, at least in part, the pathogenic mechanism of bacteremia observed during streptococcal toxic shock syndrome caused by group A Streptococcus (GAS). We have extended this study by assaying the in vitro adhesion of these same isolates to mammalian cells. Unexpectedly, we found that high-virulence GAS isolates in the mouse model showed low adhesion to the host cells. Similarly, the rate of recovery from the peritoneal cavity and cardiac blood of mice after intraperitonial injection was higher for high- than for low-virulence strains. Levels of expression of molecules that affect the adhesion of GAS to host cells were not significantly correlated with GAS virulence. Taken together, these results indicate that the invasiveness of GAS, reflected as higher virulence, is correlated directly with lower adhesion to host cells.

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Section: Resultssupporting

confidence: 89%

Positive correlation between low adhesion of group AStreptococcusto mammalian cells and virulence in a mouse model

Miyoshi‐Akiyama

Zhao

Uchiyama

et al. 2009

FEMS Microbiology Letters

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“…We also found identical PFGE patterns in GAS isolates obtained from different diseases, showing that strains displaying different genetic backgrounds have the ability to adhere, colonize and infect distinct human sites. Other authors have reported GAS isolates that display the same PFGE pattern as causes of both invasive and non-invasive disease (Descheemaeker et al , 2000; Murase et al , 1999; Nakashima et al , 1997).…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial susceptibility patterns and genomic diversity in strains of Streptococcus pyogenes isolated in 1978–1997 in different Brazilian cities

Melo

Figueiredo

Ferreira-Carvalho

2003

Journal of Medical Microbiology

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Penicillin has been the antimicrobial of choice for the treatment of Streptococcus pyogenes infections for almost six decades. Although penicillin-resistant isolates have not been described to date, clinical failures have been reported after treatment with â-lactams. In this study, we analysed the antimicrobial susceptibility and genetic diversity of S. pyogenes isolates obtained from healthy carriers or patients in different cities in the south and south east of Brazil. The MICs were determined for penicillin and seven other antimicrobials. Penicillin tolerance was also investigated. Genetic diversity was analysed by PFGE after SmaI fragmentation of the genomic DNA. All 211 isolates tested were susceptible to penicillin (MIC 0·0025-0·02 mg l À1 ). Four isolates were moderatelyMost of the other drugs tested were very active against the strains examined, except for tetracycline, to which 50 % of strains were resistant. We also found extensive genetic diversity, in that 60 different patterns were recognized in the 96 strains studied. Indeed, we found no correlation between tetracycline resistance and clonality. Despite this diversity, some PFGE patterns persisted for up to 18 years and specific clone types were spread over different geographical locations

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“…(2000) have suggested the existence of another pathway for the invasion. In addition, the worldwide resurgence in severe invasive diseases is highly linked to serotype M1 and M3 (Kiska et al ., 1997; Murase et al ., 1999), and M1 and M3 proteins have been reported to be more effective in invasion than M6 protein (Berkower et al ., 1999; Dombek et al ., 1999). In other words, serotype M1 and M3 GAS strains may induce apoptosis more efficiently in epithelial cells than other serotypes.…”

Section: Discussionmentioning

confidence: 99%

Cytochromec-mediated caspase-9 activation triggers apoptosis inStreptococcus pyogenes-infected epithelial cells

Nakagawa¹,

Nakata

Kawabata

et al. 2001

Cellular Microbiology

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Epithelial cells are the initial sites of host invasion by group A Streptococcus pyogenes (GAS), and their infection of epithelial cells has been suggested to induce apoptosis. However, the mechanism responsible for bacteria–host interaction and the induction of apoptosis has not been clearly understood. We demonstrate here that human pharyngeal epithelial HEp‐2 cells became apoptotic with DNA fragmentation by invasion of GAS strains JRS4 (M6+, F1+) and JRS145 (M6−, F1+ mutant of JRS4), whereas apoptotic cellular changes were not observed in SAM1 (M6+, F1− mutant) or SAM2 (M6−, F1− mutant) infected HEp‐2 cells. Confocal microscopy revealed that Bax translocation to mitochondria and cytochrome c release occurred after 4 h of infection. Western blot analyses showed that the amounts of Bcl‐2 and Bcl‐xL were decreased in the mitochondria of infected cells. In addition, we demonstrated that the release of nuclear histone from infected cells was prevented by the addition of caspase‐9 inhibitor (Ac‐LEHD‐CHO). We conclude that the internalization of GAS in epithelial cells is necessary and sufficient for the induction of apoptosis, which is initiated by mitochondrial dysfunction, and the mechanism of GAS‐induced apoptosis is clearly different from that induced by other intracellular invasive bacteria, e.g. Shigella and Salmonella species.

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Characteristics of Streptococcus pyogenes Serotype M1 and M3 Isolates from Patients in Japan from 1981 to 1997

Cited by 17 publications

References 18 publications

Positive correlation between low adhesion of group AStreptococcusto mammalian cells and virulence in a mouse model

Positive correlation between low adhesion of group AStreptococcusto mammalian cells and virulence in a mouse model

Antimicrobial susceptibility patterns and genomic diversity in strains of Streptococcus pyogenes isolated in 1978–1997 in different Brazilian cities

Cytochromec-mediated caspase-9 activation triggers apoptosis inStreptococcus pyogenes-infected epithelial cells

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