Characteristics of yorkshire swine natural killer cells

Pinto, Angelo J.; Ferguson, Frederick G.

doi:10.1016/0165-2427(88)90022-0

Cited by 19 publications

(18 citation statements)

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“…During Porcine Pregnancy E a r l y p r e g n a n c y -a s s o c i a t e d p o r c i n e endometrium (GD15-28) is enriched two to three fold in uterine natural killer (uNK) cells [34] [34,35]. Using monoclonal antibodies against specific surface markers, Bischof and his colleagues showed that porcine uterine lymphocytes were packed beneath the luminal epithelium, around glands and blood vessels and scattered throughout the stroma [36,37].…”

Section: Recruitment Of Uterine Natural Killer Cellsmentioning

confidence: 99%

A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

Tayade

Yuan

Croy

2007

Journal of Reproduction and Development

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Abstract. Meat pig breeds used commercially in North America lose significant numbers of geneticallynormal fetuses in the peri-implantation (attachment) period and at mid-gestation (day 50 of the114 day gestation interval). Fetal demand that is in excess to the placental blood supply is thought to underlie these waves of fetal loss. In many species, the endometrium of early normal pregnancy is enriched in innate immune cells, particularly uterine natural killer (uNK) cells. In pigs, a species with epitheliochorial placentation, conceptuses mediate about a three-fold enrichment in uNK cells at attachment sites but the functions of these cells are unknown. In species with hemochorial placentation, uNK cells are highly enriched during the process of decidualization and promote endometrial angiogenesis. We have conducted molecular analyses using pure samples of endometrial lymphocytes or endothelium and trophoblast from healthy and arresting conceptus attachment sites in Yorkshire gilts immediately post-attachment [gestation day (GD) 20] and at mid pregnancy (GD50). In healthy sites, angiogenesis was more robustly promoted by lymphocytes than by trophoblasts. An early sign of impending fetal arrest was loss of vascular endothelial growth factor (VEGF) transcription from the lymphocytes and elevation in transcription of the pro-inflammatory gene Interferon (IFN)-γ. We have postulated that newly differentiated endometrial endothelial cells, not fetal trophoblasts, are damaged by the maternal withdrawal of vascular support and onset of inflammation and that this endometrial damage contributes significantly to peri-implantation fetal death.

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Section: Recruitment Of Uterine Natural Killer Cellsmentioning

confidence: 99%

A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

Tayade

Yuan

Croy

2007

Journal of Reproduction and Development

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“…Pigs younger than 4 weeks have little or no lytic acitivity against the NK-sensitive target cells, K562, possibly reflecting the influence of normal microbial flora in accelerating NK cell maturation [15]. Pig NK cells differ from those of other species in that in vitro assays of lytic activity require 16-hour incubation periods [11]. Delayed lysis of target cells by pig NK cells is not due to delayed target recognition but to refractoriness in lysis itself which may be due to the lack of cytoplasmic granules in pig NK cells [11].…”

Section: Introductionmentioning

confidence: 99%

“…Pig NK cells differ from those of other species in that in vitro assays of lytic activity require 16-hour incubation periods [11]. Delayed lysis of target cells by pig NK cells is not due to delayed target recognition but to refractoriness in lysis itself which may be due to the lack of cytoplasmic granules in pig NK cells [11].…”

Section: Introductionmentioning

confidence: 99%

“…Porcine NK cells are CD2+FcÁR+ lymphocytes with subsets that are also CD8+ [12][13][14]. NK cell-mediated lytic activities are highest in the peripheral blood and spleen cells, lower in lymph node cells and not expressed by thymic cells [11]. Pigs younger than 4 weeks have little or no lytic acitivity against the NK-sensitive target cells, K562, possibly reflecting the influence of normal microbial flora in accelerating NK cell maturation [15].…”

Section: Introductionmentioning

confidence: 99%

“…In Yorkshire pigs, NK cells are small to medium-sized, non-granular, non-adherent lymphocytes [11]. Porcine NK cells are CD2+FcÁR+ lymphocytes with subsets that are also CD8+ [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Natural Killer Cell Frequency and Function in Pigs Selectively Bred for High or Low Antibody and Cell-Mediated Immune Response: Response to Vaccination with Modified Live Transmissible Gastroenteritis Virus

Raymond¹,

Wilkie²

1998

Nat Immun

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Porcine NK cells are small to medium lymphocytes which are lytic for tumours and virally infected cells when co-cultured for long periods (≈16 h). The frequency and function of NK cells were examined in generation 8 of pigs genetically selected for high (H), low (L), and control (C) antibody (Ab) and cell-mediated immune response (CMIR). The NK phenotype was identified using a pan-species NK-specific murine monoclonal antibody (5C6) and both binding and lysis of the NK target, K562. Vaccination with modified live transmissible gastroenteritis virus (TGEV) had no effect on blood leucocyte NK cell frequency. In interactions with K562 targets, NK cells of H and C pigs responded similarly in frequency of conjugate formation and lytic activity, while L pigs had very little or no response. Therefore, in pigs selected for high Ab and CMIR, there was no correlated enhancement of NK-cell-related traits following vaccination with TGEV, while selection for low immune response was associated with reduced NK response in pigs. This may suggest that low immune response can reflect reduced contribution of NK cells in pigs.

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Design of a flow cytometric assay for the determination of natural killer and cytotoxic T-lymphocyte activity in human and in different animal species

et al. 2000

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Background The most common assay used to detect natural killer (NK) and cytotoxic T‐lymphocyte (CTL) activity is the 51Cr release assay. The numerous disadvantages of this method led us to evaluate cytotoxicity functions by flow cytometry. We described a flow cytometric assay to assess NK and CTL activity from different species. Methods This assay is based on a dual fluorescent staining of target cells. The dye, DIOC18(3) (3,3′‐dioctadecyloxacarbocyanine perchlorate), is used to stain the membrane of different target cells. Propidium iodide (PI) is used to label dead target and effector cells. This labeling allows a clear discrimination between both cell populations. Results A good correlation was observed between the percentage of target lysis and the effector‐to‐target cell (E/T) ratios with human and porcine peripheral blood mononuclear cells (PBMC) as effector cells. The flow cytometric assay was shown to be as sensitive and as reliable as the 51Cr release performed with human cells. The assay was also applied successfully to measure NK cell activity in other animal species (pig, rabbit, hen, and mouse) and to measure murine CTL activity against the influenza virus. Conclusions We provide evidence that the flow cytometric assay using DIOC18(3) is highly reproducible and is suitable to measure different types of cell cytotoxicity. Cytometry 41:289–297, 2000. © 2000 Wiley‐Liss, Inc.

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Characteristics of yorkshire swine natural killer cells

Cited by 19 publications

References 18 publications

A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

A Review of Gene Expression in Porcine Endometrial Lymphocytes, Endothelium and Trophoblast During Pregnancy Success and Failure

Natural Killer Cell Frequency and Function in Pigs Selectively Bred for High or Low Antibody and Cell-Mediated Immune Response: Response to Vaccination with Modified Live Transmissible Gastroenteritis Virus

Design of a flow cytometric assay for the determination of natural killer and cytotoxic T-lymphocyte activity in human and in different animal species

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