Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel

Abstract

A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into a soft hydrogel network on a poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal hydrogel was molecularly characterized via Coomassie and Lowry assay protein staining agents as well as by X-ray photoelectron spectroscopy. The bactericidal activity was established against Staphylococcus aureus and Staphylococcus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain further insight into the biological stability, the hydrogels were incubated with human serum prior to activity testing without loss of activity. These studies revealed a promising bactericidal hydrogel with good stability under physiological conditions.

Antimicrobial hydrogels: promising materials for medical application

“…237,238 A Gram-positive antibacterial activity possessing peptide (KIGAKI)3-NH2 with hairpin and self-assembly structure was incorporated with hydrogels by Liu et al 239 Highly active AMP CKRWWKWIRW-NH2 was immobilized to the surface of poly(ethylene terephthalate) hydrogel, thus establishing bactericidal activity against S. aureus and S. epidermidis. 240 Poly-lysine, a popular AMP that has been reported by Zhou et al, was applied in photopolymerized antimicrobial hydrogels, which can be promising coatings for medical devices and implants (Figure 7). 241,242 In the research studies conducted by Jiang et al, 243 cationic multidomain peptides (MDPs) demonstrated a better antimicrobial activity in hydrogels than in solution.…”

Nisin/polyanion layer-by-layer films exhibiting different mechanisms in antimicrobial efficacy

“…DX-nisin lm. Since nisin was not released from DX-nisin lm, the activity of (DX-nisin) 60 lm against S. epidermidis was examined using modied Japanese Industrial Standard test JIS Z 2801:2000 35,36 for surface antibacterial efficacy. Fresh overnight grown 10 9 CFU mL À1 bacterial suspension in LB medium was diluted with PBS to obtain 10 5 CFU mL À1 .…”

Self-assembling ultrashort NSAID-peptide nanosponges: multifunctional antimicrobial and anti-inflammatory materials

“…Displaying multiple modes of intracellular and extracellular action, antimicrobial peptides demonstrate a reduced propensity for developing antimicrobial resistance and are a significant prospect for solving the current shortage of antimicrobials in pharmaceutical development [13]. Several research groups have studied their incorporation into synthetic hydrogel matrices for the prevention of biomaterial and wound infections with promising results thus far [14,15,16]. Hydrogels possessing inherent antimicrobial properties represent a significant benefit by reducing microbial contamination within the matrix and at the wound/implantation site [17].…”