Characterization and application of Lippia alba (Mill) and Cymbopogon citratus D.C. Stapf. essential oils as natural sanitizers in coriander

Abstract

Essential oils (EOs) are a promising alternative for food preservation and can be used instead of synthetic antimicrobial compounds such as sanitizers, as long as they are efficient and safe. On view of this possibility of use, Lippia alba (Mill) (LAEO) and Cymbopogon citratus D.C. Stapf. (CCEO) essential oils were evaluated as natural antimicrobials and applied into coriander for its biocontrol. The EOs were extracted and their physical properties, total phenolics, cytotoxicity to Artemia salina L. and antimicrobial potential in vitro and on natural coriander microbiota were determined. The EOs had a LD50 (median lethal dose-checked), are considered biologically active. MOC (Minimum Inhibitory Concentration) varied from 1.25 to 5.00 μL/ mL and from 0.62 to 5.00 μL/ mL for LAEO and CCEO, respectively, on standard strains of Staphylococcus aureus, Salmonella Thyphimurium, Salmonella Enteritidis, Escherichia coli and Listeria monocytogenes. The lowest MOC values for LAEO and CCEO were selected for application in coriander. EOs reduced the pathogenic microbial load in the coriander revealing its potential as a natural sanitizer, and thus demonstrating superior or equivalent efficacy to commercial sanitizer. By correlating the cytotoxic and antimicrobial results obtained, we have shown that these EOs present potential for application in foods.

Hair loss improvement effect of Chrysanthemum zawadskii, peppermint and Glycyrrhiza glabra herbal mixture in human follicle dermal papilla cell and C57BL/6 mice

“…Our previous study demonstrated the antioxidant and anti-inflammatory synergistic effects of the complex FHH-CZ (Cho et al, 2021). Studies on the synergistic effect of bioactivity by mixing natural materials have been reported (Ozkan et al, 2021;Park et al, 2021;Lima et al, 2019).…”

A Review of Essential Oils as Antimicrobials in Foods with Special Emphasis on Fresh Produce

“…de Lima et al (24) studied the biocidal potential of bushy matgrass and lemongrass EOs in aqueous solutions with T20 (0.1%, w/v) against strains of foodborne pathogens and natural microbiota on coriander. Lemongrass EO had the lowest MIC of the two EOs (0.62 μL/mL) against S. aureus, Salmonella Typhimurium, Salmonella Enteritidis, and L. monocytogenes.…”

Chemical constituents, larvicidal activity and molluscicidal from fresh leaves of Alpinia zerumbet (Pers.) and Cymbopogon citratus (DC.) Stapf

“…LC 50 for EO action front Artemia salina L.According to table 3, the EO of C. citratus presented LC 50 equivalent to 315.12 mg•L -1 , being classified as nontoxic according to the criterion of[22] that standardizes LC 50 ≥ 250 mg•L -1 of the EO as nontoxic. Lima et al[56] evaluated the toxicity of the methanol extract of medicinal plants according to the A. salina toxicity bioassay, found LC 50 equivalent to 704.67 ± 31.44 μg•mL -1 , classifying the methanolic extract of the leaves of C. citratus as nontoxic. Divergentresults were found by Ribeir et al[57] when analyzing the toxicity of EO C. citratus against A. salina in the form of a lethal dose (LD 50 ) quantified in 18.85 (μg•mL -1 ), containing variations in the limits of 13.71 to 26 (μg•mL -1 ).…”