Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica

Gabbai-Armelin, Paulo Roberto; Kido, Hueliton Wilian; Cruz, Matheus Almeida; Prado, João P. S.; Avanzi, Ingrid Regina; Custódio, Márcio R.; Rennó, Ana Cláudia Muniz; Granito, Renata Neves

doi:10.1007/s10126-018-9858-9

Cited by 21 publications

(26 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BS treated tibial bone defect presented an accelerated material degradation, with an intense ingrowth of granulation tissue into the defect area [25][26][27][28]. It is well known that silica ion is an important element for bone formation stimulation [17,29] and interaction with bone tissue, allowing the formation of a silica gel layer. This layer constitutes a template for calcium and phosphate deposition, which attracts macrophages, mesenchimal stem cells and osteoprogenitor cells [30,31].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Biological Effects of Marine Biosilica on a Tibial Bone Defect in Rats

Cruz

Gabbai-Armelin

Santana

et al. 2020

Braz. arch. biol. technol.

Self Cite

View full text Add to dashboard Cite

Research on biomaterials of natural origin has gained prominence in the literature. Above all, marine sponges, due to their architecture and structural components, present a promising potential for the engineering of bone tissue. In vitro studies demonstrate that a biosilica of marine sponges has osteogenic potential. However, in vivo works are needed to elucidate the interaction of biosilica (BS) and bone tissue. The objective of the study was to evaluate the morphological and chemical characteristics of BS compared to Bioglass (BG) by scanning electron microscopy (SEM) and X-ray dispersive energy (EDX) spectroscopy. In addition, to evaluate the biological effects of BS, through an experimental model of tibial bone defect using histopathological, histomorphometric, immunohistochemical (IHC) and mechanical tests. SEM and EDX demonstrated the successful extraction of BS. Histopathological analysis demonstrated that Control Group (GC) had greater formation of newly formed bone tissue compared to BG and BS, yet BG bone neoformation was greater than BS. However, BS showed material degradation and granulation tissue formation, with HIGHLIGHTS  BS was isolated from specimens of the marine sponge Tedania ignis collected in the north coast of São Paulo, Brazil. BS derived from marine sponges has been used as a promising raw material for bone grafts substitutes and tissue engineering proposes. The present work successfully obtained crystalline BS from marine sponges, able of properly interacting with bone tissue. The results are very inspiring toward further in vivo investigations. Cruz, M. A..; et al.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vivo Biological Effects of Marine Biosilica on a Tibial Bone Defect in Rats

Cruz

Gabbai-Armelin

Santana

et al. 2020

Braz. arch. biol. technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In all the cases the cell metabolic activity was between 80 and 100% confirming their non-cytotoxic character. In addition, Gabbai-Armelin et al (2019) showed that extracts from biosilica obtained from Tedania ignis and Dragmacidon reticulatum marine sponges, at a concentration of 50 mg/mL, promoted the increment of cell viability. Our results showed that the presence of biosilica from the GB, SN, AI, and PV sponges is noncytotoxic.…”

Section: Evaluation Of Cytotoxicitymentioning

confidence: 99%

Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges

et al. 2021

View full text Add to dashboard Cite

Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).

show abstract

“…For characterization of the BCM, the samples were dried at room temperature in a laminar flow cabinet and covered with coverslip [25]. Finally, the dried BCM membranes were mounted on carbon tapes and aluminum stubs, sputtercoated and analyzed by SEM at 1000x and 1200x magnification for checking the surface on both sides of the BCM.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Bacterial cellulose membrane enriched with fibroblast growth factor associated with photobiomodulation: In vitro evaluation

Quintana¹,

Prado²,

França³

et al. 2021

World J. Adv. Res. Rev.

View full text Add to dashboard Cite

One promising skin substitutes in the wound healing are the bacterial cellulose membranes (BCM). These biomaterials present nanostructures composed of microfibrils capable of forming three-dimensional pores that allow cell. In association with these biopolymers, several treatments are used, such as enrichment by growth factors and/or the application of photobiomodulation (PBM). Therefore, the aim of this study was to investigate the viability, proliferation and cytotoxicity of a BCM (culturing of Komagataeibacter xylinus), with or without FGF-2 in association with PBM therapy. In the characterization of BCM we saw that the membrane does not show great variations in pH and with the scanning electron microscopy it was possible to observe that the BCM has a denser and a porous side that allows the adhesion of fibroblasts, confirmed by histological staining and DAPI/Phalloidin. In vitro evaluation showed that the immunofluorescence (CaAM/EthD-1) for live and dead cells presented, in the groups with combined treatment at long-term of PBM and FGF-2, a greater quantity of live cells than with these isolated treatments and/or at short-term. However, in the short-term of combined treatment PBM and FGF-2 supplementation, fibroblasts and macrophages were more viable by Alamar Blue, in direct and indirect contact respectively. The comet assay did not show cytotoxicity for DNA damage in fibroblasts indirect contact with membrane extract. The results highlight the potential of association of FGF-2 supplementation with the application of PBM for use with BCM, due to its promoted increased cell density at long-term and improved viability in fibroblasts and macrophages at short-term.

show abstract

Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica

Cited by 21 publications

References 56 publications

In Vivo Biological Effects of Marine Biosilica on a Tibial Bone Defect in Rats

In Vivo Biological Effects of Marine Biosilica on a Tibial Bone Defect in Rats

Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges

Bacterial cellulose membrane enriched with fibroblast growth factor associated with photobiomodulation: In vitro evaluation

Contact Info

Product

Resources

About