Characterization and emulsifying property of a novel bioemulsifier by Aeribacillus pallidus YM-1

Abstract

Aims: Biosurfactants and bioemulsifiers commonly have the advantages of biodegradability, low toxicity, selectivity and biocompatibility over chemically synthesized surfactants. The goal of the study is to present a novel bioemulsifier with great application potential. Methods and Results: Aeribacillus pallidus YM‐1, isolated from crude oil contaminated soil, was found to produce a novel high molecular bioemulsifier with an emulsification index of 60 ± 1% without remarkable surface tension reduction (45·7 ± 0·1 mN m−1). The number‐average molecular weight was determined as 526 369 Da by gel permeation chromatography analysis. Bioemulsifier was subjected to FT‐IR and a complex of carbohydrates (41·1%), lipids (47·6%) and proteins (11·3%) was determined. Conclusions: The bioemulsifier of A. pallidus YM‐1 was isolated from the glucose‐based culture medium and characterized with the help of chemical analytical techniques. The bioemulsifier exhibited a promising emulsifying property for biotechnology application potential in bioremediation and microbial enhanced oil recovery. Significance and Impact of the Study: This is the first report of the bioemulsifier production by A. pallidus. The potential emulsifying activity of the bioemulsifier in the present study may be explored in various biotechnological and industrial applications.

Thermophiles as a Promising Source of Exopolysaccharides with Interesting Properties

“…Aeribacillus pallidus 418 produced two high molecular weight EPSs consisting of high variety of sugars (six for EPS 1 and seven for EPS 2) with mannose as a major component (Radchenkova et al 2013 ). Recently, Aeribacillus pallidus YM-1 was reported to produce a novel bioemulsifi er consisting of lipids (47.6 %), carbohydrates (41.1 %), and proteins (11.3 %) (Zheng et al 2012 ). Carbohydrate fraction consisted of glucose (36.6 %), altrose (30.9 %), mannose (24.4 %) and galactose (8.1 %).…”

Contributions of biosurfactants to natural or induced bioremediation

“…They are a frequent object of study, as the number of publications dedicated to the isolation and subsequent characterization of novel biosurfactant-producers is constantly growing (Ferhat et al 2011; Shavandi et al 2011; Zheng et al 2012; Luna et al 2013). This fact may be attributed to an evolved approach towards industrial production, which favors both environmental awareness and sustainability through use of renewable resources (Mukherjee and Das 2010).…”

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

“…GC-MS analysis of lipidic fraction of bioemulsifier of Aeribacillus pallidus YM-1showed that hexadecanoic acid and octadecanoic acid were the major fatty acids that account for 94.4% of total fatty acids. Other fatty acids determined at lower extent were dodecanoic acid and tetradecanoic acid (Zheng et al, 2012).…”