Characterization and Expression Analyses of Callose Synthase Enzyme (Cals) Family Genes in Maize (Zea mays L.)

Niu, Qunkai; Zhang, Peng; Su, Shuai; Jiang, Bin; Liu, Xiaowei; Li, Chuan; Yu, Tao; Yi, Hongyang; Tang, Jin; Cao, Mengji

doi:10.1007/s10528-021-10103-5

Cited by 6 publications

(1 citation statement)

References 41 publications

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“…GSL proteins have high molecular weight, containing 14–16 transmembrane domains grouped at the N‐terminal and C‐terminal regions, which leaves a central hydrophilic loop facing the cytoplasm (Chen & Kim, 2009; Schneider et al, 2016). The GSL genes have already been investigated in several species, such as grapevine (Yu et al, 2016), Citrus sinensis (Granato et al, 2019), Brassica rapa (Pu et al, 2019), Physcomitrella patens (Schuette et al, 2009), Zea mays (Niu et al, 2021), and Arabidopsis thaliana (Richmond & Somerville, 2000; Verma & Hong, 2001).…”

Section: Introductionmentioning

confidence: 99%

Flagellin and mannitol modulate callose biosynthesis and deposition in soybean seedlings

Sangi

Olimpio

Coelho

et al. 2023

Physiologia Plantarum

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Callose is a polymer deposited on the cell wall and is necessary for plant growth and development. Callose is synthesized by genes from the glucan synthase‐like family (GSL) and dynamically responds to various types of stress. Callose can inhibit pathogenic infection, in the case of biotic stresses, and maintain cell turgor and stiffen the plant cell wall in abiotic stresses. Here, we report the identification of 23 GSL genes (GmGSL) in the soybean genome. We performed phylogenetic analyses, gene structure prediction, duplication patterns, and expression profiles on several RNA‐Seq libraries. Our analyses show that WGD/Segmental duplication contributed to expanding this gene family in soybean. Next, we analyzed the callose responses in soybean under abiotic and biotic stresses. The data show that callose is induced by both osmotic stress and flagellin 22 (flg22) and is related to the activity of β‐1,3‐glucanases. By using RT‐qPCR, we evaluated the expression of GSL genes during the treatment of soybean roots with mannitol and flg22. The GmGSL23 gene was upregulated in seedlings treated with osmotic stress or flg22, showing the essential role of this gene in the soybean defense response to pathogenic organisms and osmotic stress. Our results provide an important understanding of the role of callose deposition and regulation of GSL genes in response to osmotic stress and flg22 infection in soybean seedlings.

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