Characterization and fine mapping of a new early leaf senescence mutant es3(t) in rice

Su, Yan; Hu, Shikai; Zhang, Bin; Ye, Weijun; Niu, Yaofang; Guo, Longbiao; Qian, Qian

doi:10.1007/s10725-016-0219-2

Cited by 18 publications

(14 citation statements)

References 56 publications

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“…Leaf senescence is a genetically controlled developmental process that can be modulated by a variety of phytohormones, especially ABA, that plays a critical role in leaf senescence [ 31 , 32 ]. We thus further examined the senescence symptoms of detached leaves after 5 d ABA treatment.…”

Section: Resultsmentioning

confidence: 99%

Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)

2018

IJMS

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Premature leaf senescence negatively impacts the grain yield in the important monocot rice (Oryza sativa L.); to understand the molecular mechanism we carried out a screen for mutants with premature senescence leaves in a mutant bank generated by ethyl methane sulfonate (EMS) mutagenesis of elite indica rice ZhongJian100. Five premature senescence leaf (psl15, psl50, psl89, psl117 and psl270) mutants were identified with distinct yellowish phenotypes on leaves starting from the tillering stage to final maturation. Moreover, these mutants exhibited significantly increased malonaldehyde content, decreased chlorophyll content, reduced numbers of chloroplast and grana thylakoid, altered photosynthetic ability and expression of photosynthesis-related genes. Furthermore, the expression of senescence-related indicator OsI57 was significantly up-regulated in four mutants. Histochemical analysis indicated that cell death and reactive oxygen species (ROS) accumulation occurred in the mutants with altered activities of ROS scavenging enzymes. Both darkness and abscisic acid (ABA) treatments could induce leaf senescence and resulted in up- or down-regulation of ABA metabolism-related genes in the mutants. Genetic analysis indicated that all the premature senescence leaf mutants were controlled by single non-allelic recessive genes. The data suggested that mechanisms underlying premature leaf senescence are likely different among the mutants. The present study would facilitate us to further fine mapping, cloning and functional characterization of the corresponding genes mediating the premature leaf senescence in rice.

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Section: Resultsmentioning

confidence: 99%

Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)

2018

IJMS

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“…This indicates that the plants responded actively to the ROS production and transferred O 2− into H 2 O 2 but the other scavenger CAT did not increase and as a consequences H 2 O 2 accumulated in the es5 mutant leaves and execution of senescence phenotype. Higher SOD and lower CAT level were also found in the es3(t) and es4 mutants (Su et al 2016;Wang et al 2019).…”

Section: Discussionmentioning

confidence: 87%

ES5 is involved in the regulation of phosphatidylserine synthesis and impacts on early senescence in rice (Oryza sativa L.)

Rani

Liu

et al. 2020

Plant Mol Biol

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Leaf senescence, which affects plant growth and yield in rice, is an ideal target for crop improvement and remarkable advances have been made to identify the mechanism underlying this process. We have characterized an early senile mutant es5 (early leaf senescence 5) in rice exhibiting leaf yellowing phenotype after the 4-leaf stage. This phenotype was confirmed by the higher accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), the disintegration of chloroplasts, reduction in chlorophyll content and photosynthetic rate and up-regulation of senescence-associated genes (SAGs) like Osh36, OsI57, and OsI85. Positional cloning revealed that the es5 phenotype is the result of one base substitution in ES5, encoding phosphatidylserine synthase (PSS) family protein, which is involved in the base-exchange type reaction to synthesize the minor membrane phospholipid phosphatidylserine. Functional complementation of ES5 in the es5 plants completely restored the wild-type phenotype. Ultra-high-performance liquid chromatography (UHPLC) analysis showed that es5 plants had increased levels of phosphatidylserine (PS) and decreased level of phosphatidylcholine (PC). These results provide evidence about the role of PS in rice leaf senescence. Key message The ES5 locus was cloned by map-based cloning that encodes a phosphatidylserine synthase. Mutation in ES5 caused accumulation of phosphatidylserine which influences premature leaf senescence in rice (Oryza sativa L.).

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“…AAA-type ATPases constitute a large protein family in a diverse range of organisms, and thus exhibit multiple and diverse cellular functions [15,33]. In plants, AAA-ATPase genes have been implicated in proteolysis [33], male meiosis [34], vacuolar maintenance [35], peroxisome biogenesis [36], morphogenesis [37], leaf senescence [29,38], and stress [28,39] and immune responses [18][19][20][21][22]. In this study, we present a novel rice AAA-ATPase gene member, OsAAA-ATPase1.…”

Section: Discussionmentioning

confidence: 99%

Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance

Liu

Inoue

Tang

et al. 2020

IJMS

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Fatty acids (FAs) have been implicated in signaling roles in plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and the leaf-blight bacterium Xanthomonas oryzae pv. oryzae (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1–6) upregulated in the OsSSI2-kd plants, in addition to other well-known defense-related genes. Here, we report the functional analysis of OsAAA-ATPase1 in rice’s defense response to M. oryzae. Recombinant OsAAA-ATPase1 synthesized in Escherichia coli showed ATPase activity. OsAAA-ATPase1 transcription was induced by exogenous treatment with a functional analogue of salicylic acid (SA), benzothiadiazole (BTH), but not by other plant hormones tested. The transcription of OsAAA-ATPase1 was also highly induced in response to M. oryzae infection in an SA-dependent manner, as gene induction was significantly attenuated in a transgenic rice line expressing a bacterial gene (nahG) encoding salicylate hydroxylase. Overexpression of OsAAA-ATPase1 significantly enhanced pathogenesis-related gene expression and the resistance to M. oryzae; conversely, RNAi-mediated suppression of this gene compromised this resistance. These results suggest that OsAAA-APTase1 plays an important role in SA-mediated defense responses against blast fungus M. oryzae.

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Characterization and fine mapping of a new early leaf senescence mutant es3(t) in rice

Cited by 18 publications

References 56 publications

Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)

Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)

ES5 is involved in the regulation of phosphatidylserine synthesis and impacts on early senescence in rice (Oryza sativa L.)

Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance

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