Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)

Abstract

The β‐adrenergic‐like octopamine receptor (OA2B2), which binds the biogenic amine octopamine, belongs to the class of G‐protein coupled receptors and significantly regulates many physiological and behavioral processes in insects. In this study, the putative open reading frame sequence of the MsOA2B2 gene in Mythimna separata was cloned, the full‐length complementary DNA was 1191 bp and it encoded a 396‐amino acid protein (GenBank accession number MN822800). Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. Real‐time quantitative polymerase chain reaction of spatial and temporal expression analysis revealed that the MsOAB2 gene was expressed in all developmental stages of M. separata and was most abundant in egg stages and second and fourth instars compared with other developmental stages, while the expression level during the pupal stage was much lower than that at the other stages. Further analysis with sixth instar M. separata larvae showed that the MsOA2B2 gene was expressed 1.81 times higher in the head than in integument and gut tissues. Dietary ingestion of dsMsOA2B2 significantly reduced the messenger RNA level of MsOA2B2 and decreased mortality following amitraz treatment. This study provides both a pharmacological characterization and the gene expression patterns of OA2B2 in M. separata, facilitating further research for insecticides using MsOA2B2 as a target.

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

“…5). This finding agrees with the general perception of BARs as being preferentially expressed in the neurons of the central nervous system and is consistent with previous studies in insects 89–93 . Most genes showed significantly high expression in the hemolymph tissues (Fig.…”

Octopamine receptor genes are involved in the starvation response ofRhopalosiphum padi(Hemiptera: Aphididae)

“…In the phylogenetic tree, RpOAMB and RpOctR were clustered with α‐adrenergic‐like receptors from other insects; RpOctβ1R, RpOctβ2R and RpOctβ3R were clustered into β‐adrenergic‐like receptors; and RpOctR‐like was grouped with tyramine receptor‐like genes. All six OA receptors from R. padi had seven α‐helix transmembrane domains which were typical of GPCRs, which were important in the regulation of multiple insect physiological activities (Guo et al, 2021; Guo et al, 2021; Maqueira et al, 2005). The CWxP motif of three RpOctβRs in the TM6 contained the microswitch Pro, which served as a pivot for the movement of the TM6 during receptor activation (Chelikani et al, 2007; Gujar et al, 2021).…”

“…In Chilo suppressalis (Lepidoptera: Crambidae), the expression of CsOctα1R was increased in immune stress conditions (Huang et al, 2012). The significant increases in the expression of the DmOctα2R in D. melanogaster (Qi et al, 2017) and MsOA2B2 in Mythimna separate (Lepidoptera: Noctuidae) (Guo et al, 2021) were found under insecticidal stress. When Bactrocera dorsalis (Diptera: Tephritidae) larvae adults were kept under starvation, the transcript level of BdOctβR1 was increased (Li et al, 2016).…”

Knockdown of a β-Adrenergic-Like Octopamine Receptor Affects Locomotion and Reproduction of Tribolium castaneum

“…TcOctβ2R was highly expressed in the larval and old adult stages ( Figure 4 A). Similarly, DmOctβ2R , CsOctβ2R , and MsOA2B2 exhibited high expression in larvae [ 25 , 37 , 38 ]. PxOA2B2 and NiOA2B2 were highly expressed in male adults but not in female adults.…”