Characterization and Genetic Diversity of Listeria monocytogenes Isolated from Cattle Abortions in Latvia, 2013–2018

Šteingolde, Žanete; Meistere, Irēna; Avsejenko, Jeļena; Ķibilds, Juris; Bergšpica, Ieva; Streikiša, Madara; Gradovska, Silva; Alksne, Laura; Roussel, Sophie; Terentjeva, Margarita; Bērziņš, Aivars

doi:10.3390/vetsci8090195

Cited by 14 publications

(16 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Almost all new genotypes of the clinical isolates revealed during the COVID-19 pandemic in the metropolis were previously described in the works of European researchers. The L. monocytogenes diversity which related to 1185 cattle abortion cases in Latvia during the period of 2013-2018 included 27 different ST overall [74]. Four of them, ST29, 37, 451 and 7, covered more than half of the L. monocytogenes isolates; ST8, 20, 21, 391, and 425 were also detected [74].…”

Section: Discussionmentioning

confidence: 99%

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Voronina

Kunda

Рыжова

et al. 2022

Preprint

View full text Add to dashboard Cite

Invasive listeriosis is relatively rare, but is one of the deadliest food-borne infections, affecting pregnant women, their fetuses and newborn infants, the elderly and immunocompromised people. The aim of this study was to research the impact of the COVID-19 pandemic on invasive listeriosis in the metropolis. Loci and whole-genome sequencing with subsequent bioinformatic analysis were used for the study of clinical and food Listeria monocytogenes isolates revealed in 2018–2022. The results indicate the crucial change in the spectrum of the L. monocytogenes sequence types (ST) causing invasive listeriosis during the COVID-19 pandemic, with slight changes in the ST spectrum of the food isolates. An increase in sensitivity to previously non-human L. monocytogenes genotypes, namely ST8, 20, 21, 37, 391, and 425, was observed. L. monocytogenes of ST20 and 425 carried plasmids with virulence factors (VF), in addition to the 42 VF identified in the genomes with the vip gene exclusion in the genomes of ST7, 8, 21, and 37. Perinatal listeriosis cases were associated with the new hypervirulent L. monocytogenes of ST1, 4, and 219 compiled with old ST6. These data indicate the need for the more stringent control of food products for high-risk groups.

show abstract

Section: Discussionmentioning

confidence: 99%

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Voronina

Kunda

Рыжова

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The isolates showed a high molecular diversity belonging to four STs (ST7; ST12, ST37, and ST451) and six CTs (CT720; CT798; CT4399; CT9808; CT9849; CT9850). L. monocytogenes of such clonal complexes were previously isolated from food, although their prevalence was rather low [ 86 , 87 ].…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

2022

View full text Add to dashboard Cite

In the present study, 100 L. monocytogenes isolates of serogroup IIa from food and food production environments in Poland were characterized towards the presence of virulence, resistance, and stress response genes using whole-genome sequencing (WGS). The strains were also molecularly typed and compared with multi-locus sequence typing (MLST) and core genome MLST analyses. The present isolates were grouped into 6 sublineages (SLs), with the most prevalent SL155 (33 isolates), SL121 (32 isolates), and SL8 (28 isolates) and classified into six clonal complexes, with the most prevalent CC155 (33 strains), CC121 (32 isolates), and CC8 (28 strains). Furthermore, the strains were grouped to eight sequence types, with the most prevalent ST155 (33 strains), ST121 (30 isolates), and ST8 (28; strains) followed by 60 cgMLST types (CTs). WGS data showed the presence of several virulence genes or putative molecular markers playing a role in pathogenesis of listeriosis and involved in survival of L. monocytogenes in adverse environmental conditions. Some of the present strains were molecularly closely related to L. monocytogenes previously isolated in Poland. The results of the study showed that food and food production environments may be a source of L. monocytogenes of serogroup IIa with pathogenic potential.

show abstract

“…Sequence types ST29 (CC29, n = 3), ST37 (CC37, n = 3), and ST451 (CC11, n = 2; CC451, n = 1) were found to be the most frequent. The appearance of ST29, ST37, and ST451 was recently described in connection with cattle abortion cases in Latvia during 2013–2018 as well as in a study on the genetic diversity of L. monocytogenes from dairy production in the United States [ 26 , 27 ]. This may suggest that these particular STs are often associated with cattle.…”

Section: Resultsmentioning

confidence: 99%

Vacuum-Packed Steak Tartare: Prevalence of Listeria monocytogenes and Evaluation of Efficacy of ListexTM P100

Hlucháňová

Korena

Juřicová

2022

Foods

View full text Add to dashboard Cite

Steak tartare is a raw, ready-to-eat meal popular in European countries, the safety of which is often discussed due to the risk of foodborne illness. The aim of this study was to determine the prevalence of Listeria monocytogenes in vacuum-packed steak tartare from retailers in the Czech Republic, characterize the strains obtained by typing methods and to evaluate the efficacy of ListexTM P100 against L. monocytogenes artificially inoculated into steak tartare samples. The prevalence of L. monocytogenes was 55% and 17 isolates belonging mostly to serotype 1/2a were obtained. Altogether 11 sequence types and 11 clonal complexes were assigned based on the whole genome sequencing (WGS) signifying the high diversity of L. monocytogenes isolates obtained. Core genome multi-locus sequence typing (cgMLST) did not confirm an epidemiological connection with human cases of listeriosis. The efficacy of ListexTM P100 treatment at concentrations of 108 and 109 PFU/g on artificially inoculated beef steak tartare samples was not efficient. Based on the results of this study, steak tartare from retailers can be considered as a source of L. monocytogenes that remains a challenge to the food industry.

show abstract

Characterization and Genetic Diversity of Listeria monocytogenes Isolated from Cattle Abortions in Latvia, 2013–2018

Cited by 14 publications

References 52 publications

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland

Vacuum-Packed Steak Tartare: Prevalence of Listeria monocytogenes and Evaluation of Efficacy of ListexTM P100

Contact Info

Product

Resources

About