Characterization and identification of the mucilage extracted from orchid bulbs (Bletia campanulata) by high temperature capillary gas chromatography (HT‐CGC)

Abstract

Key Words:Capillary gas chromatography High temperature capillary gas chromatography Sugar analysis Derivatization of oligosaccharides . SummaryThe aim of this work was to develop an analytical method by High Temperature Capillary Gas Chromatography (HT-CGC) to characterize and identify the presence of tzauhtli, orchid mucilage, in works of art. During the pre-Hispanic and colonial period, tzauhtli was used as an adhesive for paper and feathers, as well as paint binder, and was one of the components of the paste used to model corn stalk sculptures. The mucilage was extracted with hot water from orchid bulbs (Bletiu campanulnta) and I yophilized. The free sugars from the mucilage were analyzed by CGC after derivatization. The polysaccharide of the mucilage was characterized after its partial acid hydrolysis by HT-CGC analysis of the oligosaccharides obtained. The proposed chromatographic method allows the identification of different mucilages in works of art. This contribution is the first report of the oligosaccharides from the hydrolysis of the mucilage extracted from orchid bulbs (Bletia campanulate).[I81 R. Araujo ct al., "Esculturas de papcl amate y calia de maiz". Cuadernos Tecnicos 1.Musco F r a u Mayer, Mexico (1989).

Estado da arte da cromatografia gasosa de alta resolução e alta temperatura

“…A CGAR-AT aumentou consideravelmente o âmbito dessas análises, suplantando a cromatografia por fluido supercrítico (SFC), até mesmo para a análise de oligossacarídeos considerados termolábeis 89 . Outra aplicação de CGAR-AT em análise de oligossacarí-deos é a identificação de gomas formadas por polissacarídeos em obras de arte 90 . Uma tinta pode ser considerada como uma mistura de um pigmento e uma goma.…”

Holy Corn. Interdisciplinary Study of a Mexican 16th-Century Polychrome Maize Stem, Paper, and Colorín Wood Sculpture

“…The debarked stems were ground and bound with a natural adhesive to prepare the paste for modeling the volumes. The historical references indicate the use of a polysaccharide gum named tatzingueni or tzauhtli extracted from orchid bulbs [15], but so far only animal glue has been identified in the sculptures analyzed [16].…”