1992
DOI: 10.1093/nar/20.24.6473
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Characterization and localization ofcis-diamminedichloro-platinum(II) adducts on a purified oligonucleotide containing the codons 12 and 13 ofH-rasproto-oncogene

Abstract: The use of substrates containing well defined adducts at precise sites, is required to perform a careful analysis of the toxic and mutagenic potential of a lesion. As a first step in this direction the octamer 5'-d(CCGGCGGT), containing the sequence of the codons 12 d(GGC) and 13 d(GGT) of the human H-ras gene, was reacted with the antitumoral drug cis-diamminedichloroplatinum(II). The platinated products have been purified by HPLC. A first set of experiments, including enzymatic digestions with nuclease P1 fo… Show more

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Cited by 9 publications
(4 citation statements)
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“…To gain deeper insights into the reaction products we then enzymatically digested the duplex dsODN 4 following its incubation with conjugate 1 by using nuclease P1 and Antarctic Phosphatase,20 and subsequently analyzed the resulting mixture by LC‐ESI‐HRMS. We were able to identify two DNA lesions from the mass peaks observed at around m / z = 556 and 622 (Figures 4 and 5).…”
Section: Resultsmentioning
confidence: 99%
“…To gain deeper insights into the reaction products we then enzymatically digested the duplex dsODN 4 following its incubation with conjugate 1 by using nuclease P1 and Antarctic Phosphatase,20 and subsequently analyzed the resulting mixture by LC‐ESI‐HRMS. We were able to identify two DNA lesions from the mass peaks observed at around m / z = 556 and 622 (Figures 4 and 5).…”
Section: Resultsmentioning
confidence: 99%
“…The cisplatin-modified 90-mer was prepared by ligating a 14-mer, an 8-mer containing the Pt-d(GpG) adduct purified as reported (9), and a 68-mer, using a 65-mer as scaffold. The resulting 90-mer-Pt oligonucleotide was purified on a 20% polyacrylamide͞7 M urea͞30% formamide denaturing gel and hybridized to a 5Ј 32 P-labeled 17-mer primer for ssDNA synthesis assays.…”
Section: Methodsmentioning
confidence: 99%
“…Existing synthetic strategies for preparing intrastrand cross-links have been limited mainly to direct reactions of electrophiles with oligonucleotides, making the study of these adducts in sequences containing multiple reactive sites quite challenging, if not impossible. Such strategies include hybridization with a second strand to direct the adduction (9) or HPLC separation of multiple adducts (16).…”
mentioning
confidence: 99%