Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

Seo, Jang Kyun; Lim, Won Seok; Jeong, Ji Hye; Yoo, Young Bok; Yie, Se Won; Kim, Kook Hyung

doi:10.5423/ppj.2004.20.3.200

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…In orchid biology, the plant-fungus symbiotic partnership is critical, but the roles viruses may play in this relationship remain largely unknown. Most mycoviruses appear to have little influence on fungal pathogenicity (Seo et al, 2004;Vainio et al, 2012), but some are demonstrated to influence their hosts. The most well-known example of a mycovirus that reduces fungal pathogenicity is CHV1 of Cryphonectria parasitica, the fungal pathogen that causes chestnut blight (Anagnostakis & Day, 1979).…”

Section: Viruses Fungi and Orchidsmentioning

confidence: 99%

Novel and divergent viruses associated with Australian orchid-fungus symbioses

Ong

Sivasithamparam

et al. 2018

Virus Research

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Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups.

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Section: Viruses Fungi and Orchidsmentioning

confidence: 99%

Novel and divergent viruses associated with Australian orchid-fungus symbioses

Ong

Sivasithamparam

et al. 2018

Virus Research

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“…However, there have been no clear evidences to suggest a relationship between the reduction of yield or abnormal growth of oyster mushroom and the presence of viruses. Numerous fungal viruses have recently been reported from all genera of fungi, including the oyster mushrooms (Yu et al, 2003;Seo et al, 2004;Lim et al, 2005). Some of these whole or partial virus genes were cloned, and their sequences were reported.…”

Section: J Microbiolmentioning

confidence: 99%

The identification of a novel Pleurotus ostreatus dsRNA virus and determination of the distribution of viruses in mushroom spores

Kim

et al. 2008

J Microbiol.

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Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses.

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