Characterization and Utilization of Candida rugosa Lipase Immobilized on Controlled Pore Silica

Soares, Cleide Mara Faria; Castro, Heizir F. de; Moraes, Flávio Faria de; Zanin, Gisella Maria

doi:10.1007/978-1-4612-1604-9_68

Cited by 106 publications

(129 citation statements)

References 22 publications

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“…The recovered immobilized lipase was then washed with tert-butanol to remove any substrate or product retained in the matrix. Hydrolytic activity was determined by the olive oil emulsion method according to the modification proposed by Soares et al (1999). The inactivation constant (kd) and half-life (t1/2) for the immobilized lipase were calculated as described by Costa-Silva et al, (2014).…”

Section: Operational Stability Of the Immobilized Derivativementioning

confidence: 99%

Performance of an enzymatic packed bed reactor running on babassu oil to yield fatty ethyl esters (FAEE) in a solvent-free system

et al. 2015

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HIGHLIGHTS The performance of packed bed reactor running on babassu oil to yield ethyl esters was assessed. Burkholderia cepacia lipase immobilized on SiO2-PVA composite was used as a catalyst. The molar ratio of babassu oil and ethanol was found as a critical parameter for attaining high yields. The transesterification reaction of babassu oil with ethanol mediated by Burkholderia cepacia lipase immobilized on SiO2-PVA composite was assessed in a packed bed reactor running in the continuous mode. Experiments were performed in a solvent-free system at 50 °C. The performance of the reactor (14 mm ×210 mm) was evaluated using babassu oil and ethanol at two molar ratios of 1:7 and 1:12, respectively, and operational limits in terms of substrate flow rate were determined. The system's performance was quantified for different flow rates corresponding to space times between 7 and 13 h. Under each condition, the impact of the space time on the ethyl esters formation, the transesterification yield and productivity were determined. The oil to ethanol molar ratio was found as a critical parameter in the conversion of babassu oil into the correspondent ethyl esters. The highest transesterification yield of 96.0 ± 0.9% and productivity of 41.1 ± 1.6 mgestergcatalystwere achieved at the oil to ethanol molar ratio of 1:12 and for space times equal or higher than 11 h. Moreover, the immobilized lipase was found stable with respect to its catalytic characteristics, exhibiting a half-life of 32 d.

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Section: Operational Stability Of the Immobilized Derivativementioning

confidence: 99%

Performance of an enzymatic packed bed reactor running on babassu oil to yield fatty ethyl esters (FAEE) in a solvent-free system

et al. 2015

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“…A atividade lipolítica das amostras foi detectada utilizando-se a metodologia descrita por Soares et al (1999). Através da reação de 5 mL de emulsão de óleo de oliva e goma arábica (7 %), 2 mL de tampão fosfato de sódio (0,1 M), pH 7,0 e 1 mL do líquido metabólico.…”

Section: Determinação Da Atividade Lipolíticaunclassified

Avaliação Do Potencial Biotecnológico De Aspergillus Parasiticus Ucp 1281 No Biotratamento De Efluentes Da Indústria De Laticínios E Produção De Lipídeos

Reis

Leão

Souza

et al. 2015

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RESUMO: Neste trabalho foi realizada a identificação de Aspergillus sp (SIS 16), isolado de amostra do solo da região da Caatinga no estado de Pernambuco, e avaliado o potencial biotecnológico deste, no biotratamento de efluentes da indústria láctea. O isolado foi identificado, através das características macroscópicas e microscópicas como A. parasiticus (UCP1281). (MONTEIRO, 2012;LIU et al., 2012;SMRITHI et al., 2013;MONCIARDINI et al., 2014).A Caatinga é um ecossistema do sertão nordestino.Estende-se por cerca de 735.000 km 2 , sendo limitada a leste e a oeste pelas florestas Atlântica e Amazônica, respectivamente, e ao sul pelo Cerrado (REIS, 1976). Apresenta um clima semi-árido, quente e com baixos índices pluviométricos, baixa umidade relativa do ar e altas temperaturas durante quase todo o ano (COSTA et al., 2014 (LIU et al., 2008;NOTARNICOLA et al., 2012; JOSHI; WALIA; RANA, 2012; HOSSEINPOUR et al., 2012;PANESAR, KENNEDY, 2012;LIN et al., 2013;WANG et al., 2013;SUNG, 2013; AGGELOPOULOS et al., 2014;PLEISSNER et al., 2014;MARQUES et al., 2014 IDENTIFICAÇÃO E CARACTERIZAÇÃO MORFOLÓGICAA identificação e a caracterização morfológica da amostra foram realizadas através da metodologia descrita por Klick (2002), por meio da chave para identificação da espécie. A amostra foi cultivada nos meios Ágar Czapek autolisado de levedura -(CYE) (PITT, 1979) a 25 °C e 37 °C, e Extrato de Malte Àgar (MEA) a 25 °C. As características macroscópicas foram determinadas pela textura da colônia, grau de esporulação, cor dos conídios, textura e cor do micélio, presença de cores, pigmentos e exsudado.Para análise microscópica, foram preparadas lâminas, para observação das estruturas microscópicas como:comprimento, largura e textura dos conidióforos, forma da cabeça conidial, diâmetro da vesícula, comprimento das métulas e fiálides, diâmetro, forma, textura presença ou ausência de conídios e ascósporos, forma e cor do cleistotécio/esclerócios. PRÉ-INOCULOForam utilizadas placas de Petri contendo o meio ágar Sabouraud (acrescido de azeite de oliva para aclimatação) e incubadas a 28 ºC durante 7 dias. CINÉTICA DE CRESCIMENTO E DE PRODUÇÃO DE LIPASE EM MEIO CONVENCIONAL DETERMINAÇÃO DA BIOMASSA MICROBIANAA determinação da biomassa foi feita através de gravimetria. A massa micelial foi lavada e filtrada em papel de filtro, transferida para a estufa a 50 °C para secagem, em seguida transferida para o dessecador até peso constante. DETERMINAÇÃO DA ATIVIDADE LIPOLÍTICAA atividade lipolítica das amostras foi detectada utilizando-se a metodologia descrita por Soares et al. (1999). Através da reação de 5 mL de emulsão de óleo de oliva e goma arábica (7 %), 2 mL de tampão fosfato de sódio (0,1 M), pH 7,0 e 1 mL do líquido metabólico. A mistura foi agitada em agitador orbital a 82 rpm, 37 ºC, durante 10 minutos.A reação foi paralisada através da adição de 10 mL de EXTRAÇÃO E QUANTIFICAÇÃO DOS LIPÍDEOS TOTAISOs lipídeos totais da biomassa foram extraídos utilizando-se 1,0 g da biomassa liofilizada que foi submetida à extração com a mistur...

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“…The hydrolytic activities of the free and immobilized lipase derivatives were assayed by the olive oil emulsion method according to the modification proposed by Soares et al (1999). The substrate was prepared by mixing 50 ml of olive oil with 50 ml of gum arabic solution (7% w/v).…”

Section: Hydrolytic Activity Determinationmentioning

confidence: 99%

“…This reaction was selected because it gave measurable results with the maximum accuracy in a short span of time and with a minimum amount of lipase. In addition, this reaction system has been used by Soares et al (1999); Oliveira et al (2000) and Pereira et al (2003). Under these conditions the MML immobilized on the POS-PVA showed higher synthetic activity than the CRL immobilized derivative (Table 2).…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

“…Thus, exploiting good immobilization carriers has been an attractive work for the enzyme engineering. Several methods for lipase immobilization have been reported, including deposition on to solid supports (Oliveira et al, 2000;Person et al, 2000), covalent binding (Soares et al, 1999) and entrapment within a polymer matrix or hydrophobic sol-gel materials (Keeling-Tucker et al, 2000;Reetz et al, 1998). The latter method can be applied to a variety of enzymes, yielding immobilized systems with 80-fold esterification activity compared to the free enzyme (Reetz et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles

Bruno

Filho

Castro

2008

Braz. arch. biol. technol.

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Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activitieswere found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also obtained through chemical composition (FTIR).

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Characterization and Utilization of Candida rugosa Lipase Immobilized on Controlled Pore Silica

Cited by 106 publications

References 22 publications

Performance of an enzymatic packed bed reactor running on babassu oil to yield fatty ethyl esters (FAEE) in a solvent-free system

Performance of an enzymatic packed bed reactor running on babassu oil to yield fatty ethyl esters (FAEE) in a solvent-free system

Avaliação Do Potencial Biotecnológico De Aspergillus Parasiticus Ucp 1281 No Biotratamento De Efluentes Da Indústria De Laticínios E Produção De Lipídeos

Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles

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