Characterization of a Battery of Monoclonal Antibodies for Differentiation of Newcastle Disease Virus and Pigeon Paramyxovirus-1 Strains

Dp, Lana; Db, Snyder; Dj, King; Ww, Marquardt

doi:10.2307/1590814

Cited by 52 publications

(24 citation statements)

References 19 publications

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“…Several MoAbs enable characterization of isolates as non-virulent (Jestin et al, 1989;Lana et al, 1988;Meulemans et al, 1987;Russell et al, 1988). However, up to now, no MoAbs have been described which enable characterization of isolates as virulent.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

et al. 1997

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Section: Discussionmentioning

confidence: 99%

Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

et al. 1997

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“…MAb B79 reacts in all serologic assays with an antigenic site common to all serotype 1 avian paramyxoviruses. AVS is a paramyxovirus 1 lentogenic strain-specific MAb, which reacts with avirulent strains only, and was used as a negative control (24). Equal amounts of purified parental and mutant recombinant viruses were used as antigens.…”

Section: Cells and Virusesmentioning

confidence: 99%

“…The mutant rG1, rG3, and rG12 viruses showed higher rates of reactivity than the other mutants with MAb B79, and the rG1 and rG3 viruses showed higher reactivity to MAb 15C4 than the parental rBC virus and other mutant viruses. The antibody binding sites are unknown for these MAbs, but they were shown to be neutralizing (24). Hence, enhanced recognition of HN by antibodies B79 and 15C4 by these viruses is important in the context of eliciting a neutralizing antibody response in the host.…”

Section: Generation Of Recombinant Ndv Containing Hn N-linked Glycosymentioning

confidence: 99%

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Panda

Elankumaran

Krishnamurthy

et al. 2004

J Virol

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The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) is an important determinant of its virulence. We investigated the role of each of the four functional N-linked glycosylation sites (G1 to G4) of the HN glycoprotein of NDV on its pathogenicity. The N-linked glycosylation sites G1 to G4 at residues 119, 341, 433, and 481, respectively, of a moderately pathogenic NDV strain Beaudette C (BC) were eliminated individually by site-directed mutagenesis on a full-length cDNA clone of BC. A double mutant (G12) was also created by eliminating the first and second glycosylation sites at residues 119 and 341, respectively. Infectious virus was recovered from each of the cDNA clones of the HN glycoprotein mutants, employing a reverse genetics technique. There was a greater delay in the replication of G4 and G12 mutant viruses than in the parental virus. Loss of glycosylation does not affect the receptor recognition by HN glycoprotein of NDV. The neuraminidase activity of G4 and G12 mutant viruses and the fusogenicity of the G4 mutant virus were significantly lower than those of the parental virus. The fusogenicity of the double mutant virus (G12) was significantly higher than that of the parental virus. Cell surface expression of the G4 virus HN was significantly lower than that of the parental virus. The antigenic reactivities of the mutants to a panel of monoclonal antibodies against the HN protein indicated that removal of glycosylation from the HN protein increased (G1, G3, and G12) or decreased (G2 and G4) the formation of antigenic sites, depending on their location. In standard tests to assess virulence in chickens, all of the glycosylation mutants were less virulent than the parental BC virus, but the G4 and G12 mutants were the least virulent.

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“…mAbs may detect slight variations in antigenicity, such as single amino acid changes at the epitope to which the antibody is directed. The use of mAb technology provided a new approach to antigenic differentiation of NDV strains and isolates, and several groups developed mAbs against NDV strains, primarily for diagnostic purposes (Hoshi et al, 1983;Nishikawa et al, 1983;Russell & Alexander, 1983;Alexander et al, 1985aAlexander et al, , 1987Ishida et al, 1985;Abenes et al, 1986;Srinivasappa et al, 1986;Erdei et al, 1987;Meulemans et al, 1987;Lana et al, 1988;Jestin et al, 1989). mAbs to NDV have been effective in the following areas.…”

Section: Use Of Monoclonal Antibodies In the Diagnosis Of Ndmentioning

confidence: 99%

Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)

2001

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Substantial variation in the virulence of Newcastle disease virus (NDV) isolates means that the detection of NDV or evidence of infection is insufficient for an adequate diagnosis, as control measures for avirulent viruses are very different to those for virulent viruses. Diagnosis therefore requires further characterization, at least as to whether an isolate is virulent or avirulent. Conventional detection and differentiation of ND viruses is perceived as slow, laborious and requiring an undesirable use of in vivo techniques. In addition, further characterization is needed to give greater information on origin and spread. This review concentrates on the application of monoclonal antibody and molecular biological approaches. Panels of monoclonal antibodies were a major advance for the characterization of NDV isolates, although confirmation of virulence for poultry still required in vivo testing. As molecular-based techniques become easier and more reliable, they are likely to supersede the use of monoclonal antibodies, especially for characterizing viruses for epidemiological purposes. The attraction of molecular-based techniques is that they may be able to cover all three aspects of Newcastle disease diagnosis (detection of virus, characterization, including inference of virulence, and epidemiology) quickly, accurately and definitively in a single test. A number of approaches based on the reverse transcriptase polymerase chain reaction have been developed, with subsequent analysis of the product by restriction enzyme analysis, probe hybridization and nucleotide sequencing. Although extensive variation among NDVs still poses technical problems, the real and potential advantages of a molecular biological approach to Newcastle disease diagnosis appear to be overwhelming.

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Characterization of a Battery of Monoclonal Antibodies for Differentiation of Newcastle Disease Virus and Pigeon Paramyxovirus-1 Strains

Cited by 52 publications

References 19 publications

Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

Differentiation of virulent and non‐virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)

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