Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic and propionic acids

Fujita, Masanori; Ike, Michihiko; Tachibana, Shinya; Kitada, Go; Kim, Shin Myoung; Inoue, Zensuke

doi:10.1016/s1389-1723(00)88048-2

Cited by 111 publications

(56 citation statements)

References 33 publications

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“…Polyacrylamide displayed the highest flocculating activity, of 93.19%. , 1997;Yim et al, 2007;Lu et al, 2005;Suh et al, 1997;Salehizadeh et al, 2000;Nakata and Kurane, 1999;Fujita et al, 2000) but reports on consortia of microorganisms are still very scarce in the literature. From this study we observed a good flocculating activity in the bioflocculant produced by the consortium of Cobetia sp.…”

Section: Wastewater Flocculation Assaymentioning

confidence: 99%

Characteristics of a bioflocculant produced by a consortium of Cobetia and Bacillus species and its application in the treatment of wastewaters

Ugbenyen¹,

Okoh²

2014

WSA

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The characteristics of a bioflocculant produced by a consortium of 2 bacteria belonging to the genera Cobetia and Bacillus was investigated. The extracellular bioflocculant was composed of 66% uronic acid and 31% protein and showed an optimum flocculation (90% flocculating activity) of kaolin suspension at a dosage of 0.8 mg/mℓ, pH of 8, and with Ca 2+ as a coagulant aid. The bioflocculant is thermally stable, with a high residual flocculating activity of 86.7%, 89.3% and 87% after heating at 50 o C, 80 o C and 100 o C, respectively, for 25 min. FTIR analysis of the bioflocculant indicated the presence of hydroxyl, amino, carbonyl and carboxyl functional groups. Scanning electron microscopy (SEM) revealed a crystal-linear sponge-like bioflocculant structure and EDX analysis of purified bioflocculant indicated an elemental composition in mass proportions of C:N:O:S:P of 6.67:6.23:37.55:0.38:4.42 (% w/w). The produced bioflocculant was highly efficient in removing turbidity and reducing chemical oxygen demand (COD) in brewery wastewater, dairy wastewater and river water. The bioflocculant could flocculate kaolin clay more efficiently than traditional flocculants; alum and polyethylenimine.

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Section: Wastewater Flocculation Assaymentioning

confidence: 99%

Characteristics of a bioflocculant produced by a consortium of Cobetia and Bacillus species and its application in the treatment of wastewaters

Ugbenyen¹,

Okoh²

2014

WSA

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“…Many types of bioflocculants originating from bacteria have been discovered. Some of them showed efficient flocculating activities on not only inorganic but also organic suspended particles (Fujita et al 2000). For example, REA-11 from Corynebacterium glutamicum and flocculant of Aspergillus sojae AJ-7002 showed high activity in the removal of color from dye effluent and the removal of microorganisms from activated sludge, respectively (He et al 2002(He et al , 2004Nakamura et al 1976).…”

Section: Introductionmentioning

confidence: 99%

Bioflocculant produced by Chlamydomonas reinhardtii

Chen

Zhao

et al. 2011

J Appl Phycol

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Bioflocculants of Chlamydomonas reinhardtii were investigated under axenic conditions. C. reinhardtii was found to produce significant amounts of bioflocculants. Flocculating activity by C. reinhardtii began in the linear phase of growth and continued until the end of the stationary phase. The highest flocculating efficiency of the culture broth was 97.06%. The purified C. reinhardtii bioflocculant was composed of 42.1% (w/w) proteins, 48.3% carbohydrates, 8.7% lipids, and 0.01% nucleic acid. The optimum condition for bioflocculant production of C. reinhardtii was as follows: under temperature of 15°C to 25°C, pH 6-10 and illumination of 40-60 μmol photons m −2 s −1 . The bioflocculants produced by C. reinhardtii showed maximum activity in pH ranges from 2 to 10. The flocculating activity was significantly enhanced by the addition of CaCl 2 as a coflocculant at an optimal concentration of 4.5 mM.

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“…The CP of C. freundii IFO13545 produced from acetate has a very similar structure to that of chitin/chitosan [4,26]. Therefore, commercially available authentic chitosan from shrimp shells (Sigma-Aldrich) was used to make standard solutions for this assay.…”

Section: Resultsmentioning

confidence: 99%

Simple ELISA using Polyclonal Antibody and Lectin for Determination of Soluble Chitosan - Like Polysaccharide in Bacterial Culture

Takeo¹,

Kimura²

2017

IJBMBS

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Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic and propionic acids

Cited by 111 publications

References 33 publications

Characteristics of a bioflocculant produced by a consortium of <i>Cobetia</i> and <i>Bacillus</i> species and its application in the treatment of wastewaters

Characteristics of a bioflocculant produced by a consortium of <i>Cobetia</i> and <i>Bacillus</i> species and its application in the treatment of wastewaters

Bioflocculant produced by Chlamydomonas reinhardtii

Simple ELISA using Polyclonal Antibody and Lectin for Determination of Soluble Chitosan - Like Polysaccharide in Bacterial Culture

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