Characterization of a new cucurbit-infecting ipomovirus from Sudan

Desbiez, Cécile; Verdin, Eric; Tepfer, Mark; Wipf-Scheibel, Catherine; Millot, Pauline; Dafalla, Gasim; Lecoq, Hervé

doi:10.1007/s00705-016-2981-x

Cited by 17 publications

(17 citation statements)

References 10 publications

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“…Relatedly, in the newly described CoCMov, another Ipomovirus which we have shown to contain a DAG motif in its coat protein gene ( Fig 3 ), very low transmission with B . tabaci MED species was reported [ 85 ]. Transmission of CoCMov with the aphid species Myzus persicae was not successful, however detailed data was not shown.…”

Section: Discussionmentioning

confidence: 99%

Unusual occurrence of a DAG motif in the Ipomovirus Cassava brown streak virus and implications for its vector transmission

et al. 2017

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Cassava is the main staple food for over 800 million people globally. Its production in eastern Africa is being constrained by two devastating Ipomoviruses that cause cassava brown streak disease (CBSD); Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), with up to 100% yield loss for smallholder farmers in the region. To date, vector studies have not resulted in reproducible and highly efficient transmission of CBSV and UCBSV. Most virus transmission studies have used Bemisia tabaci (whitefly), but a maximum of 41% U/CBSV transmission efficiency has been documented for this vector. With the advent of next generation sequencing, researchers are generating whole genome sequences for both CBSV and UCBSV from throughout eastern Africa. Our initial goal for this study was to characterize U/CBSV whole genomes from CBSD symptomatic cassava plants sampled in Kenya. We have generated 8 new whole genomes (3 CBSV and 5 UCBSV) from Kenya, and in the process of analyzing these genomes together with 26 previously published sequences, we uncovered the aphid transmission associated DAG motif within coat protein genes of all CBSV whole genomes at amino acid positions 52–54, but not in UCBSV. Upon further investigation, the DAG motif was also found at the same positions in two other Ipomoviruses: Squash vein yellowing virus (SqVYV), Coccinia mottle virus (CocMoV). Until this study, the highly-conserved DAG motif, which is associated with aphid transmission was only noticed once, in SqVYV but discounted as being of minimal importance. This study represents the first comprehensive look at Ipomovirus genomes to determine the extent of DAG motif presence and significance for vector relations. The presence of this motif suggests that aphids could potentially be a vector of CBSV, SqVYV and CocMov. Further transmission and ipomoviral protein evolutionary studies are needed to confirm this hypothesis.

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Section: Discussionmentioning

confidence: 99%

Unusual occurrence of a DAG motif in the Ipomovirus Cassava brown streak virus and implications for its vector transmission

et al. 2017

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“…Phylogenetic analyses aligned SPMMV closer to tritimoviruses than to other potyvirids (Stenger et al, 1998) and, as expected, SPMMV HCPro lacks sequence similarity with potyviral HCPros outside the protease domain. Ipomoviruses without HCPro have one P1 copy (Mbanzibwa et al, 2009) or two divergent P1 copies in tandem (Desbiez et al, 2016;Janssen et al, 2005;Li et al, 2008;Valli et al, 2006) at the N-terminal part of the viral polyprotein. Remarkably, as in the case of SPMMV, all ipomoviruses lacking HCPro use P1 as an RSS.…”

Section: The Diversity Of Hcpro and Hcpro-like Proteins-similar But Dmentioning

confidence: 99%

The HCPro from thePotyviridaefamily: an enviable multitasking Helper Component that every virus would like to have

Vallí

Gallo

Rodamilans

et al. 2017

Molecular Plant Pathology

178

142

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RNA viruses have very compact genomes and so provide a unique opportunity to study how evolution works to optimize the use of very limited genomic information. A widespread viral strategy to solve this issue concerning the coding space relies on the expression of proteins with multiple functions. Members of the family Potyviridae, the most abundant group of RNA viruses in plants, offer several attractive examples of viral factors which play roles in diverse infection-related pathways. The Helper Component Proteinase (HCPro) is an essential and well-characterized multitasking protein for which at least three independent functions have been described: (i) viral plant-to-plant transmission; (ii) polyprotein maturation; and (iii) RNA silencing suppression. Moreover, multitudes of host factors have been found to interact with HCPro. Intriguingly, most of these partners have not been ascribed to any of the HCPro roles during the infectious cycle, supporting the idea that this protein might play even more roles than those already established. In this comprehensive review, we attempt to summarize our current knowledge about HCPro and its already attributed and putative novel roles, and to discuss the similarities and differences regarding this factor in members of this important viral family.

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“…This suggests that CVYV has been present for a long time in sub‐Saharan Africa, with few genetic exchanges with Mediterranean populations of the virus. The frequency of CVYV in Sudan was high among cultivated cucurbits – almost 25% of the collected and tested samples – whereas the closely related CocMoV, transmitted by the same vector and infecting the same hosts in the laboratory, had a very low frequency in the same surveys and was found only twice in the perennial cucurbit weed Coccinia grandis (Desbiez et al , ). These contrasted situations could be related to different adaptations to the local biotype(s) of B. tabaci , and/or to the relative geographic isolation of the area where CocMoV is found.…”

Section: Discussionmentioning

confidence: 99%

“…Cucurbit samples, mostly presenting mosaics, deformation, vein clearing or yellowing symptoms suggestive of virus infections (Lecoq et al , , ; Desbiez et al , ) were collected in Sudan between 1992 and 2012. All samples ground in phosphate solution (Na 2 HPO 4 0.03 m + diethyldithiocarbamate 0.2%) were stored at −20 °C and used when needed for RNA extraction and RT‐PCR.…”

Section: Methodsmentioning

confidence: 99%

“…CVYV has been observed so far throughout the Middle East and the Mediterranean Basin (EPPO, ), and also in Sudan (Desbiez et al , ) and possibly India (Ghosh & Mukhopadhyay, ), but not in eastern Asia or America (Lecoq & Desbiez, ). In addition, ipomoviruses infecting cucurbits related to, but distinct from, CVYV have been described in diverse geographic locations: squash vein yellowing virus (SqVYV) in North and Central America (Adkins et al , ) and in Israel (Reingold et al , ) and coccinia mottle virus (CocMoV) in Sudan (Desbiez et al , ). CVYV is now spreading all over the Mediterranean Basin, as a result of the increasingly high populations of its vector Bemisia tabaci (Lecoq & Desbiez, ).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for long‐term prevalence of cucumber vein yellowing virus in Sudan and genetic variation of the virus in Sudan and the Mediterranean Basin

Desbiez¹,

Caciagli²,

Wipf-Scheibel³

et al. 2019

Plant Pathology

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Cucumber vein yellowing virus (CVYV) is emerging throughout the Mediterranean Basin, where it causes significant damage to cucumber and melon crops. It has been suggested that CVYV originated from the Middle East and has spread only recently to other areas. In this work, an isolate from Sudan was characterized, and surveys performed in that country between 1992 and 2012 revealed a long‐term presence of CVYV with a high molecular variability, showing that the virus has long been endemic in sub‐Saharan Africa. Comparison of the full‐length sequences of 11 CVYV isolates from different geographic origins revealed recombination events in CVYV populations from the Mediterranean Basin and the Middle East, and evidence for different selection pressures along the genome. These results shed a new light on the evolution of CVYV.

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Characterization of a new cucurbit-infecting ipomovirus from Sudan

Cited by 17 publications

References 10 publications

Unusual occurrence of a DAG motif in the Ipomovirus Cassava brown streak virus and implications for its vector transmission

Unusual occurrence of a DAG motif in the Ipomovirus Cassava brown streak virus and implications for its vector transmission

The HCPro from thePotyviridaefamily: an enviable multitasking Helper Component that every virus would like to have

Evidence for long‐term prevalence of cucumber vein yellowing virus in Sudan and genetic variation of the virus in Sudan and the Mediterranean Basin

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