Characterization of a novel porcine enterovirus in wild boars in Hungary

Abstract

Porcine enteroviruses (PEVs) are members of the family Picornaviridae, genus Enterovirus. Until now, only three different PEV genotypes (PEV-9 and -10, and PEV-3H/PEV-14) have been detected in domestic pigs, and there is no information about the presence of PEVs in wild animals. Here, we identify and characterize the complete genomes of PEV originated from 5 of 10 (50%) of wild boar (Sus scrofa) piglets by RT-PCR and pyrosequencing. Wild boar/WBD/2011/HUN (JN807387) PEV showed only 67% amino acid identity in VP1 compared to the most closely related prototype PEV-3H/PEV-14. Wild boar enterovirus represents a novel PEV genotype, provisionally called PEV-15.

Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam

“…In contrast, detection of EV RNA in boars in this study was much lower than the 50 and 69.4 % prevalence reported in Hungary (Boros et al, 2012a) and the Czech Republic (Prodělalová, 2012), respectively. High rates of EV infection in young pigs (detection rate w90 %) suggested that these viruses maintain endemic transmission in this population.…”

“…Further studies on their genetic relatedness led to the reassignment of these viruses into three separate picornavirus genera: Enterovirus, Sapelovirus and Teschovirus (Kaku et al, 2001;Knowles, 2006;Krumbholz et al, 2002;Zell et al, 2001). Of these, PEV-9 and PEV-10 were classified as EV-G1 and EV-G2 serotypes in enterovirus G, with the subsequently characterized EV-G types EV-G3-EV-G6 reported in single publications from Hungary and South Korea (Boros et al, 2011(Boros et al, , 2012aMoon et al, 2012). EV-G7 was subsequently discovered from domestic sheep by A. Boros and N. J.…”

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

“…The present study detected PTV, PSV, and EV-G more frequently in animals up to 7 months of age relative to adult (2-to 4-year-old) wild boars. Another research group reported high frequencies of positive results for EV-G and PTV infections in 6-and 8-week-old wild boars (Boros et al 2012a, Boros et al 2012b). Due to the lack of data, the results from these studies represent supplementary information that should be used in further age distribution investigations of porcine enteric picornavirus infections in wild boars.…”

“…The similarities among the results presented herein, in the Czech and Spanish studies may indicate that wild boars are more susceptible to infection by EV-G and PTV. In addition, Boros et al (2012a) proposed a novel EV-G genotype, provisionally named PEV-15, after characterizing the complete genome sequence of an EV-G strain from a wild boar. This same research group also suggested that there may be PTV strains well-adapted or specific to wild boar host species (Boros et al 2012b).…”

“…Wild boars are susceptible to infections by viruses that also affect domestic pigs (Sus scrofa domesticus), such as porcine group A rotavirus (PoRVA), porcine circovirus type 2 (PCV2), hepatitis E virus (HEV), Aujeszky disease virus (ADV), porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSF), torque teno sus virus (TTSuV), influenza A virus, and others (Ruiz-Fons et al 2008, Meng et al 2009, Okadera et al 2013, Biondo et al 2014. These animals are also susceptible to infections by PTV, PSV, and EV-G, which were described in asymptomatic wild boars in Hungary (Boros et al 2012a, Boros et al 2012b, Spain (Cano--Gómez et al 2013), and the Czech Republic (Prodělalová 2012).…”