“…3 D1S8 locus on chromosome 1 contains three classes of variant 29 bp repeat units: a-type, t-type and (rarely) unamplified null or O-type repeats. 4,5 When analyzed electrophoretically using two tracks a and t for the analysis of atype and t-type units, respectively, MVR-PCR of total genomic DNA produces a profile of both alleles superimposed to generate six different types for each "rung" of the ladder; an intense band in the a track with no band in the t track (code 1, aa; called homozygote), an intense band in the t track with no band in the a track (code 2, tt; called homozygote), a faint a band with a faint t band (code 3, at; called heterozygote), a faint a band without t band (code 4, a0; called heterozygote), a faint t band without a band (code 5, t0; called heterozygote) and no band in either a or t track (code 6, 00). Code 4 and code 5 were distinguished from code 1 and code 2 by their intensities of bands, respectively.…”