Characterization of a Water-insoluble Polymer Producing Bacterium &lt;i&gt;Enterobacter&lt;/i&gt; sp. CJF-002 for MEOR

Otsuka, Makiko; Fujiwara, Kazuhiro; Sugai, Yuichi; Kishita, Atsushi; Enomoto, Heiji; Chida, Tadashi; Yazawa, Nintoku; Nagase, Keiji; Hong, Chengxie; Ji, Cui

doi:10.1627/jpi.47.282

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…[51], and Enterobacter spp. [52]. BC production has also been reported in species such as Agrobacterium tumefaciens [53] and Escherichia coli [54].…”

Section: Bc Producing Microorganismsmentioning

confidence: 99%

Bacterial cellulose: Strategies for its production in the context of bioeconomy

et al. 2022

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Bacterial cellulose has advantages over plant‐derived cellulose, which make its use for industrial applications easier and more profitable. Its intrinsic properties have been stimulating the global biopolymer market, with strong growth expectations in the coming years. Several bacterial species are capable of producing bacterial cellulose under different culture conditions; in this context, strategies aimed at metabolic engineering and several possibilities of carbon sources have provided opportunities for the bacterial cellulose's biotechnological exploration. In this article, an overview of biosynthesis pathways in different carbon sources for the main producing microorganisms, metabolic flux under different growth conditions, and their influence on the structural and functional characteristics of bacterial cellulose is provided. In addition, the main industrial applications and ways to reduce costs and optimize its production using alternative sources are discussed, contributing to new insights on the exploitation of this biomaterial in the context of the bioeconomy.

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“…[51], and Enterobacter spp. [52]. BC production has also been reported in species such as Agrobacterium tumefaciens [53] and Escherichia coli [54].…”

Section: Bc Producing Microorganismsmentioning

confidence: 99%

Bacterial cellulose: Strategies for its production in the context of bioeconomy

et al. 2022

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“…Since the first report of cellulose synthesis in bacteria (Brown 1886), the list of bacteria where cellulose production has been demonstrated, and the number of bacteria where genes for cellulose biosynthesis have been identified, has grown longer. In addition to the acetic acid bacteria that includes species of Gluconacetobacter, production of cellulose is demonstrated in bacterial species such as Agrobacterium tumefaciens (Matthysse et al 1981), E. coli (Thongsomboon et al 2018), Rhizobium (Napoli et al 1975), Enterobacter (Otsuka et al 2004) and others. Interestingly, analysis of genome sequences of a large number of bacteria led to identification of genes for cellulose biosynthesis in many more bacterial species than are known to produce cellulose.…”

Section: An Overview Of Cellulose Synthesis In Bacteriamentioning

confidence: 99%

“…In addition to plants, other organisms, including bacteria, produce cellulose as an extracellular polysaccharide. Cellulose biosynthesis is reported in a number of bacterial and cyanobacterial species (Napoli et al 1975;Matthysse et al 1981;Nobles et al 2001;Otsuka et al 2004;Kumagai et al 2011;Kawano et al 2011;Castro et al 2013; Thongsomboon et al 2018), but it is in the acetic acid bacteria where production of cellulose is best observed and studied. Even among the acetic acid bacteria, it is Gluconacetobacter xylinus/Gluconacetobacter hansenii (referred to as Acetobacter xylinum in earlier publications, and more recently classified in the genus Komagataeibacter) (Yamada and Yukphan 2008;Yamada et al 2012) that have been studied extensively for cellulose biosynthesis, and where maximal amount of cellulose production is observed.…”

Section: Introductionmentioning

confidence: 99%

Cellulose-synthesizing machinery in bacteria

et al. 2021

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Cellulose is produced by all plants and a number of other organisms, including bacteria. The most representative cellulose-producing bacterial species is Gluconacetobacter xylinus (G. xylinus), an acetic acid bacterium. Cellulose produced by G. xylinus, commonly referred to as bacterial cellulose (BC), has exceptional physicochemical properties resulting in its use in a variety of applications. All cellulose-producing organisms that synthesize cellulose microfibrils have membrane-localized protein complexes (also called terminal complexes or TCs) that contain the enzyme cellulose synthase and other proteins. The bacterium G. xylinus is a prolific cellulose producer and a model organism for studies on cellulose biosynthesis.The widths of cellulose fibers produced by Gluconacetobacter are 50-100 nm, suggesting that cellulose-synthesizing complexes are nanomachines spinning a nanofiber. At least four different proteins (BcsA, BcsB, BcsC, and BcsD) are included in TC from Gluconacetobacter, and the proposed function of each is as follows: BcsA, synthesis of a glucan chain through glycosyl transfer from UDP-glucose; BcsB, complexes with BcsA for cellulose synthase activity; BcsC, formation of a pore in the outer membrane through which a glucan chain is extruded; BcsD, regulates aggregation of glucan chains through four tunnel-like structures. In this review, we discuss structures and functions of these four and a few other proteins that have a role in cellulose biosynthesis in bacteria.

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“…14 The RC fibers, such as Rayon, Cupra, and Lyocell, were prepared from their solutions; therefore, to control the structure and mechanical properties of the final cellulose products, the structure formation is important especially for RC. In this study, the effects of dyestuffs on the structure formation of both natural cellulose, BC from Enterobacter species, 15,16 and RC prepared from a cuprammonium hydroxide solution, which is the dope solution of Cupra, was investigated mainly by focusing on the effect in the crystallinity and structure formation.…”

mentioning

confidence: 99%

“…In this study, the effects of dyestuffs on the structure formation of both natural cellulose, BC from Enterobacter species, 15,16 and RC prepared from a cuprammonium hydroxide solution, which is the dope solution of Cupra, was investigated mainly by focusing on the effect in the crystallinity and structure formation.…”

mentioning

confidence: 99%

Influence of dyestuffs on the crystallinity of a bacterial cellulose and a regenerated cellulose

Miyamoto

Tsuduki

Ago

et al. 2014

Textile Research Journal

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The crystal structures of bacterial cellulose (BC) obtained by cultivation of an Enterobacter species CJF 002 stock under the presence of direct, acid, and basic dyestuffs were examined. Optical microscopic observation showed that direct and basic dyestuffs stained BC samples but acid dyestuff did not. This suggests that direct and basic dyestuffs are contained within the resulting BC samples. Analysis of wide angle x-ray diffraction (WAXD) data indicates that direct dyestuffs inhibited crystallization of BC at dyestuff concentration in culture media ( Cdye) of more than 0.05 wt% with lower angle shift of the diffraction peak for the (200) plane of BC, but almost no influence on BC crystallization in the case of basic dyestuff was observed. In addition, we investigated the crystallinity of regenerated cellulose (RC) from a cuprammonium solution and the reaction of RC with the dyestuffs. The dyestuffs had almost no impact on the crystallinity of RC even in cases where the samples showed staining. It was found that the apparent crystallite size of (110) and (020) in the RC samples with dyestuffs were slightly lower than that in the RC blank sample, while the apparent crystallite size of ([Formula: see text]) in the RC samples with dyestuffs retain the values at the same level as the RC blank sample. These results suggest that the cellulose molecular sheets held together by van der Waals interactions were the basic structure formed from RC and they probably retain their structure in the cuprammonium solution at relatively high concentrations of cellulose.

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Characterization of a Water-insoluble Polymer Producing Bacterium <i>Enterobacter</i> sp. CJF-002 for MEOR

Cited by 11 publications

References 7 publications

Bacterial cellulose: Strategies for its production in the context of bioeconomy

Bacterial cellulose: Strategies for its production in the context of bioeconomy

Cellulose-synthesizing machinery in bacteria

Influence of dyestuffs on the crystallinity of a bacterial cellulose and a regenerated cellulose

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