Characterization of an Antigen from Rat Male Accessory Glands

Pesoa, Susana; Galmarini, Mirian; Pistoresi-Palencia, María C.; Riera, Clelia M.

doi:10.1159/000233936

Cited by 4 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The autoantigen appears roughly at 4 weeks of age in males. 1 Chemical modification did not change the specificity but played an adjuvant role. 2,3 The immunoregulatory mechanism involved in this model was studied by our group.v" A state of specific tolerance to MRAG was induced in syngeneic rats born from mothers immunized with a regular dose of MRAG incorporated to complete Freund's adjuvant (CFA).…”

Section: Introductionmentioning

confidence: 99%

Maternal‐Fetal Interactions on the Regulation of the Autoimmune Response

Yranzo‐Volonté¹,

Riera²

1990

American J Rep Immunol

Self Cite

View full text Add to dashboard Cite

Adult female rats were immunized with 5 mg or 25 mg of modified rat male accessory glands (MRAG) incorporated to complete Freund's adjuvant (CFA) before, during, and after pregnancy. The mothers and litters were exchanged between the experimental and normal groups. The offspring were brought up to 20 days of age and immunized with 5 mg of MRAG-CFA and 5 mg of human serum albumin (HSA)-CFA. Anti-MRAG antibodies were detected in the offspring brought up by the immunized mothers and the titers were similar to those of the foster mothers whereas in the offspring of the experimental group fostered by normal mothers antibodies to MRAG were not detected. The DTH performed in the offspring 13 days after the immunization was significantly diminished in male and female offspring from the 5 mg and 25 mg experimental group fostered by normal mothers (P less than 0.0005 for all groups). Similar results were found when the offspring from normal mothers were suckled by mothers immunized with MRAG-CFA. To assess whether MRAG or HSA administered to female rats reached the offspring via the placenta or the milk, female rats were immunized with 3 mg of 125I-MRAG-CFA or with 3 mg of 125I-HSA-CFA. When radioactivity was measured in neonates (n = 11) that were suckled by the 125I-MRAG-CFA immunized mothers, the specific activity was 116 in stomach (0.4 micrograms of MRAG) and 940 in the total organs (3.8 micrograms of MRAG).(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Introductionmentioning

confidence: 99%