Characterization of an influenza a host range mutant

Maassab, Hunein F.; DeBorde, Dan C.

doi:10.1016/0042-6822(83)90088-0

Cited by 24 publications

(14 citation statements)

References 20 publications

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“…Likewise, many genes, including the NS genes, have been implicated in the attenuation of influenza viruses in different hosts (4,25,43). However, in our system, the transfer of the NS gene segment from Eng53/v-a to PR8 was alone sufficient to confer the high-growth phenotype (Fig.…”

Section: Discussionmentioning

confidence: 56%

Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics

Ozaki¹,

Govorkova²,

et al. 2004

J Virol

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Influenza A viruses are the cause of annual epidemics of human disease with occasional outbreaks of pandemic proportions. The zoonotic nature of the disease and the vast viral reservoirs in the aquatic birds of the world mean that influenza will not easily be eradicated and that vaccines will continue to be needed. Recent technological advances in reverse genetics methods and limitations of the conventional production of vaccines by using eggs have led to a push to develop cell-based strategies to produce influenza vaccine. Although cell-based systems are being developed, barriers remain that need to be overcome if the potential of these systems is to be fully realized. These barriers include, but are not limited to, potentially poor reproducibility of viral rescue with reverse genetics systems and poor growth kinetics and yields. In this study we present a modified A/Puerto Rico/8/34 (PR8) influenza virus master strain that has improved viral rescue and growth properties in the African green monkey kidney cell line, Vero. The improved properties were mediated by the substitution of the PR8 NS gene for that of a Vero-adapted reassortant virus. The Vero growth kinetics of viruses with H1N1, H3N2, H6N1, and H9N2 hemagglutinin and neuraminidase combinations rescued on the new master strain were significantly enhanced in comparison to those of viruses with the same combinations rescued on the standard PR8 master strain. These improvements pave the way for the reproducible generation of high-yielding human and animal influenza vaccines by reverse genetics methods. Such a means of production has particular relevance to epidemic and pandemic use.

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Section: Discussionmentioning

confidence: 56%

Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics

Ozaki¹,

Govorkova²,

et al. 2004

J Virol

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“…A t l~he i n d i c a t e d times, t h e inSeeted cells were collected into retieulocytes s t a n d a r d buffer (0.01 5I KCI, 0.0015 5I MAC12, 0.01 ~ Tris-HC1 p H 7.4), a n d were e x t r a c t e d 5 to 7 t i m e s w i t h phenol-chloroform, as described previously (19). T h e e t h a n o l p r e c i p i t a t e d I~NA was desalted followed b y c h r o m a t o g r a p h y t h r o u g h P D -1 0 c o l u m n (Sephadex G-25 ~) in water.…”

Section: After a D S O R P T I O N For 30 M I N U T E S At Room T E Mmentioning

confidence: 99%

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

Odagiri

Tosaka

Ishida

et al. 1986

Archives of Virology

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The biological function of a cold-adapted (ca) mutation residing on the PB2 gene of an influenza A/Ann Arbor/6/60 (A/AA/6/60) ca variant virus in the viral replication cycle at 25 degrees C was studied. The viral polypeptide synthesis of A/AA/6/60 ca variant at 25 degrees C was evident approximately 6 hours earlier than the wild type (wt) virus and yielded twice as many products. The quantitative analysis of viral complementary RNA (cRNA), synthesized in the presence of cycloheximide, revealed that A/AA/6/60 ca variant and a single gene reassortant that contains only the PB2 gene of the ca variant with remaining genes of the wt virus produced equal amount of cRNA at 25 degrees and 33 degrees C, which was an amount approximately four fold greater than the wt virus' cRNA synthesized at 25 degrees C. These results strongly suggest that the ca mutation residing on the PB2 gene of A/AA/6/60 ca variant affects the messenger RNA synthesis at 25 degrees C in the primary transcription.

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“…Differences in the mean age and gender ratios between these two groups were not statistically signi®cant: mean age 79.3=80.0 for men, 83.4=82.3 for women, 82.6=81.4 for total subjects and gender ratio 0.30=0.35, respectively, for the vaccinated and control groups. Vaccine strains used in this study were in¯uenza A=Beijing=262=95 (H1N1), A=Sydney=05=97 (H3N2) and B=Mie=1=93 virus culture extracts with chick cell agglutinin in egg allantoic¯uid [5,6].…”

Section: Vaccination Protocolmentioning

confidence: 99%

Efficacy of influenza vaccine in the elderly in welfare nursing homes: reduction in risks of mortality and morbidity during an influenza A (H3N2) epidemic

Deguchi¹,

Tatara

2000

Journal of Medical Microbiology

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The effect of in¯uenza vaccination on the occurrence and severity of in¯uenza virus infection in a population residing in nursing homes for the elderly was studied during an in¯uenza A (H3N2) epidemic in Japan. Of 22 462 individuals living in 301 welfare nursing homes, 10 739 received either one dose (2027 subjects) or two doses (8712 subjects) of inactivated, subunit trivalent in¯uenza vaccine. During the period Nov. 1998 to March 1999, there were 950 cases of in¯uenza infection diagnosed clinically, with virus isolation or serology. There were statistically signi®cantly fewer cases of in¯uenza, hospital admissions due to severe infection and deaths due to in¯uenza in the vaccinated cohort (256 cases, 32 hospital admissions, 1 death) than in the unvaccinated controls (694 cases, 150 hospital admissions, 5 deaths; reduction rates 59.8%, 76.9% and 79.1% respectively). Vaccination was almost equally effective in those who received one dose of vaccine and those who received two doses. No serious adverse reactions to vaccination were recorded. Thus in¯uenza vaccination is safe and effective in this population, and should be an integral part of the routine care of persons aged >65 years residing in nursing homes.

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Characterization of an influenza a host range mutant

Cited by 24 publications

References 20 publications

Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics

Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics

Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene

Efficacy of influenza vaccine in the elderly in welfare nursing homes: reduction in risks of mortality and morbidity during an influenza A (H3N2) epidemic

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