Two genes encoding distinct glutamate carrier proteins of Escherichia coli B were cloned into an E. coli K-12 strain by using a cosmid vector, pHC79. One of them was the gltS gene coding for a glutamate carrier of an Na+-dependent, binding protein-independent, and glutamate-specific transport system. The content of the glutamate carrier was amplified about 25-fold in the cytoplasmic membranes from a gltS-amplified strain. The gitS gene was located in a 3.2-kilobase EcoRI-MluI fragment, and the gene product was identified as a membrane protein with gn apparent Mr of 35,000 in a minicell system. A gene designated gltP was also cloned. The transport activity of the gitP system in cytoplasmic membrane vesicles from a gltP-amplified strain was driven by respiratory substrates and was independent of the concentrations of Na+, K+, and Li'. An uncoupler, carbonylcyanide m-chlorophenylhydrazone, completely inhibited the transport activities of both systems, whereas an ionophore, mnonensin, inhibited only that of the gltS system. The Kt value for glutamate was 11 ,IM in the gltP system and 3.5 ,IM in the gltS system. L-Aspartate inhibited the glutamate transport of the gltP system but not that of the gitS system. Aspartate was taken up actively by membrane vesicles from the gltP-amplified strain, although no aspartate uptake activity was detected in membrane vesicles from a wild-type E. coli strain. These results suggest that gltP is a structural gene for a carrier protein of an Na+-independent, binding protein-independent glutamate-aspartate transport system.
The magnetic transition from the ferro- to the para-magnetic state of manganese(II) phthalocyanine (C32H16N8Mn) has been observed by measurements of the magnetic susceptibility, the magnetization and the proton magnetic resonance in the low-temperature range. The positive Weiss constants were obtained from the static susceptibility measurements and the proton magnetic measurements. Moreover, the magnetic-field dependences of the experimental results were found in the low-temperature range. All of those findings confirm the ferromagnetic behavior of this substance in the lower-temperature region. From the measurements of the frequency shift in the resonant circuit of the proton magnetic resonance apparatus, the transition temperature was determined to be 8.6 K. A possible mechanism of long-range ordering was also discussed on the basis of the crystal structure of this substance.
The static magnetic susceptibility and the ESR spectra from 1.6 to 300 K have been measured on a powder sample of the titled free radical. The broad maximum in the susceptibility which indicates an antiferromagnetic interaction has been observed at 6.9 K. The broadening of the ESR absorption line and the shift of the g-value have been found in the temperature region below Tmax, in which the susceptibility reached its round maximum. There appeared anomalies in the slope of the susceptibility, the linewidth, and the g-value versus temperature curves in the vicinity of 1.7 K. These anomalies may imply a magnetic-phase transition from the short-range ordered state to the long-range ordered state at about 1.7 K. The existence of a ferromagnetic interaction between the magnetic chains in the triphenylverdazyl radical solid is discussed on the basis of the susceptibility, the spin distribution, and the crystal structure. It is understood qualitatively that the radical with a negative spin density has a latent ferromagnetic interaction in or between the magnetic chains, and that the obsrevation of this interaction greatly depends upon the molecular and crystal structure.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.