Characterization of anti-sperm antibodies and their coding cDNA sequences by Epstein-Barr virus transformed B cell lines from lymphocytes of infertile women possessing anti-sperm antibodies

Association between Helicobacter pylori infection and the presence of anti-sperm antibodies

“…Sera with SIV > 2.0 were considered positive. ELISAsp was performed as described previously [24,25]. U-bottomed 96-well PVC plates (Becton Dickinson, Oxnard, CA, USA) were coated with 50 mL/ well 'swim-up' sperm suspension (1 £ 10 7 spermatozoa/mL) and then incubated with blocking buffer of 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) in phosphate-buffered saline (PBS).…”

Antichlamydial and Antisperm Antibodies in Patients with Chlamydial Infections

“…For the detection of ASA by ELISA (ELISAsp) spermatozoa were immobilized on U‐bottomed 96‐well PVC plates (Becton Dickinson Labware, Oxnard, CA, USA). The plates were treated with 50 μ g/mL (in distilled water), 50 μ L/well, poly‐ l ‐lysine Hydro bromide (Sigma Chemical Company, St Louis, MO, USA) for 1 hr at room temperature in order to increase their adhesive capacity 18 . The plates were coated with 50 μ L/well washed sperm suspension after ‘swim up’ (1 × 10 7 spermatozoa/mL) and fixed with 30% methanol.…”

“…Non‐specific binding was reduced by incubating with bovine blocking buffer [1% bovine serum albumin in phosphate‐buffered saline (PBS)]. Serum dilutions of 1:20 in PBS, 50 μ L/well of each serum were added to the plates and the ELISA was performed as described previously 18,19 . ELISA index I = A / B , was established, where A is the optical density (OD) of the tested serum at 492 nm (measured on a Uniscan reader, Lab systems, Espoo, Finland), and B is the mean value () of the OD of all sera from the tested group; ELISA barrier value was also introduced, where is the mean value of OD 492 nm from the tested negative control group and S.D.…”