Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen

Kim, Minjung; Chae, Dae-Han; Cho, Gyeongjun; Kim, Da-Ran; Kwak, Youn‐Sig

doi:10.5423/ppj.oa.05.2019.0154

Cited by 16 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pseudomonas syringae pv. actinidiae (Psa), a Gram-negative bacterium, can cause the bacterial canker disease and bring the most destructive disease in kiwifruit cultivation and production . Psa infected the kiwifruit plants and induced branches and vine ulcers and even stem vine death.…”

Section: Introductionmentioning

confidence: 99%

Eremophilane Sesquiterpenoids with Antibacterial and Anti-inflammatory Activities from the Endophytic Fungus Septoria rudbeckiae

Lin

Zhang

Han

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

Fourteen eremophilane sesquiterpenoids (1–14), including nine new congeners, septoreremophilanes A–I (1–9), together with three known sesquiterpenes (15–17), two known tetralone derivatives (18, 19), and two known cholesterol analogues (20, 21), were isolated from the endophytic fungus Septoria rudbeckiae. Compounds 1–6 and 7a belong to the family of the highly oxygenated eremophilane sesquiterpenoids with a 6/6/5 tricyclic system and bearing a hemiacetal moiety. The inhibitions of all metabolites against eight bacteria were estimated in vitro, and nine new metabolites (1–9) were tested for antineuroinflammatory activity. Notably, the effects of 4 against Pseudomonas syringae pv. actinidae and 20 against Bacillus cereus displayed potent inhibitory, with the MIC values of 6.25 and 6.25 μM, respectively. Further, scanning electron microscopy analyses indicated that 4 and 20 were to change the outer configuration of bacterial cells, respectively, and the investigations demonstrated that 4 and 20 may act as potential structure templates for the development of the agrochemical bactericides. Additionally, compound 6 displayed potent inhibition of NO generation in lipopolysaccharide-induced BV-2 microglial cells (IC50 = 12.0 ± 0.32 μM), and the conceivable anti-inflammatory mechanisms implicated were also investigated by molecular docking. Thus, the bioactive metabolites of the strain S. rudbeckiae may serve as a novel resource to be developed.

show abstract

Section: Introductionmentioning

confidence: 99%

Eremophilane Sesquiterpenoids with Antibacterial and Anti-inflammatory Activities from the Endophytic Fungus Septoria rudbeckiae

Lin

Zhang

Han

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Twig (1.5 g) and leave (5 g) were weighed and transferred to a 50-ml tube. Before DNA extraction, samples in a 50-ml tube were sonicated at 35 kHz with 50 ml of 1× phosphate buffered saline (PBS) buffer (10× PBS: 80 g of NaCl, 2 g of KCl, 1.44 g of Na 2 HPO 4 , 2.4 g of KH 2 PO 4 , per liter adjusted to a pH of 7.4) for 45 s and vortexed for 30 s three times (Kim et al, 2019a(Kim et al, , 2019bYao et al, 2019). The suspension was transferred to a new tube, then centrifuged at 1,968 ×g, 4°C for 20 min and the supernatant was discarded.…”

mentioning

confidence: 99%

Comparison of Bacterial Community of Healthy and Erwinia amylovora Infected Apples

et al. 2021

Self Cite

View full text Add to dashboard Cite

Fire blight disease, caused by Erwinia amylovora, could damage rosaceous plants such as apples, pears, and raspberries. In this study, we designed to understand how E. amylovora affected other bacterial communities on apple rhizosphere; twig and fruit endosphere; and leaf, and fruit episphere. Limited studies on the understanding of the microbial community of apples and changes the community structure by occurrence of the fire blight disease were conducted. As result of these experiments, the infected trees had low species richness and operational taxonomic unit diversity when compared to healthy trees. Rhizospheric bacterial communities were stable regardless of infection. But the communities in endosphere and episphere were significanlty affected by E. amylovora infection. We also found that several metabolic pathways differ significantly between infected and healthy trees. In particular, we observed differences in sugar metabolites. The finding provides that sucrose metabolites are important for colonization of E. amylovora in host tissue. Our results provide fundamental information on the microbial community structures between E. amylovora infected and uninfected trees, which will contribute to developing novel control strategies for the fire blight disease.

show abstract

“…CCM_MD2014 (soil isolate from the USA) [78], Streptomyces sp. KPB2 (isolated from the pollen of kiwi fruit from South Korea) [34], Streptomyces sp. PM-R01 (isolated from Durian fruit, Durio zibethinus, in Thailand) (GenBank: LC381944), and Streptomyces sp.…”

Section: Resultsmentioning

confidence: 99%

“…The complete genome of the nearest neighbor (Streptomyces sp. KPB2-Accession ID: CP034353.1) [34], was used as a reference. The contigs were sorted and merged into scaffolds with the help of a reference genome using MeDusa v1.6 [35].…”

Section: Methodsmentioning

confidence: 99%

Complete Genome Analysis of Undecylprodigiosin Pigment Biosynthesizing Marine Streptomyces Species Displaying Potential Bioactive Applications

Ramesh

Anwesh²,

Vinithkumar

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Marine Streptomyces species are underexplored for their pigment molecules and genes. In this study, we report the genome of the undecylprodigiosin biosynthesizing gene cluster carrying Streptomyces sp. strain BSE6.1, displaying antioxidant, antimicrobial, and staining properties. This Gram-positive obligate aerobic bacterium was isolated from the coastal sediment of the Andaman and Nicobar Islands, India. Pink to reddish pigmented colonies with whitish powdery spores on both agar and broth media are the important morphological characteristics of this bacterium. Growth tolerance to NaCl concentrations was 2 to 7%. The assembled genome of Streptomyces sp. BSE6.1 contains one linear chromosome 8.02 Mb in length with 7157 protein-coding genes, 82 tRNAs, 3 rRNAs and at least 11 gene clusters related to the synthesis of various secondary metabolites, including undecylprodigiosin. This strain carries type I, type II, and type III polyketide synthases (PKS) genes. Type I PKS gene cluster is involved in the biosynthesis of red pigment undecylprodigiosin of BSE6.1, similar to the one found in the S. coelicolor A3(2). This red pigment was reported to have various applications in the food and pharmaceutical industries. The genome of Streptomyces sp. BSE6.1 was submitted to NCBI with a BioProject ID of PRJNA514840 (Sequence Read Archive ID: SRR10849367 and Genome accession ID: CP085300).

show abstract

Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen

Cited by 16 publications

References 37 publications

Eremophilane Sesquiterpenoids with Antibacterial and Anti-inflammatory Activities from the Endophytic Fungus Septoria rudbeckiae

Eremophilane Sesquiterpenoids with Antibacterial and Anti-inflammatory Activities from the Endophytic Fungus Septoria rudbeckiae

Comparison of Bacterial Community of Healthy and Erwinia amylovora Infected Apples

Complete Genome Analysis of Undecylprodigiosin Pigment Biosynthesizing Marine Streptomyces Species Displaying Potential Bioactive Applications

Contact Info

Product

Resources

About