Characterization of antimicrobial resistance and virulence genes of Pseudomonas aeruginosa isolated from mink in China, 2011–2020

Guo, Lili; Li, Lu-mei; Li, Yan; Duan, Xiaoxiao; Liu, Yujing; Gao, Ruiyuan; Zhao, Yongda

doi:10.1016/j.micpath.2021.105323

Cited by 3 publications

(4 citation statements)

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“…Thirty-two antibiotic resistance genes (ARGs) and seventeen virulence genes were examined in all isolates. First, DNA was extracted using the standard boiling method ( Guo et al, 2022 ). Then, the target genes were amplified using polymerase chain reaction (PCR) and the sequence of primers, as well as the size of amplicons and the corresponding reference of each gene are presented in Supplementary Tables S1, S2 ( Skyberg et al, 2006 ; Sánchez-Jiménez et al, 2010 ; Akinyemi et al, 2011 ; Parvathi et al, 2011 ; Maravić et al, 2013 ; Fang et al, 2019 ; Sun et al, 2019 ; Chen et al, 2020 ; Han et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015–2021

Guo

Xiao²,

Wu³

et al. 2023

Front. Microbiol.

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IntroductionSalmonella is a ubiquitous foodborne pathogen and mainly transmitted to human farm-to-fork chain through contaminated foods of animal origin.MethodsIn this study, we investigated the serotypes, antimicrobial resistance and virulence of Salmonella from China.ResultsA total of 617 Salmonella isolates were collected from 4 major food animal species across 23 provi nces in China from 2015-2021. Highest Salmonella prevalence were observed in Guangdong (44.4%) and Sandong (23.7%). Chickens (43.0%) was shown to be the major source of Salmonella contamination, followed by pigs (34.5%) and ducks (18.5%). The number of Salmonella increased significantly from 5.51% to 27.23% during 2015–2020. S. Derby (17.3%), S. Enteritidis (13.1%) and S. Typhimurium (11.4%) were the most common serotypes among 41 serotypes identifiedin this study. Antibiotic susceptibility testing showing that the majority of the Salmonella isolates were resistant to neomycin (99.7%), tetracycline (98.1%), ampicillin (97.4%), sulfadiazine/trimethoprim (97.1%), nalidixic acid (89.1%), doxycycline (83.1%), ceftria xone (70.3%), spectinomycin (67.7%), florfenicol (60.0%), cefotaxime (52.0%) and lomefloxacin (59.8%). The rates of resistance to multiple antibiotics in S. Derby and S.Typhimurium were higher than that in S. Enteritidis. However, the rate of resistance to fosfomycin were observed from higher to lower by S. Derby, S. Enteritidis, and S. Typhimurium. Biofilm formation ability analysis found that 88.49%of the Salmonella were able to produce biofilms, of which 236 Salmonella isolates were strong biofilm producer. Among the 26 types of antibiotics resistance genes (ARGs) were identified in this study, 4 ARGs (tetB,sul2,aadA2, and aph(3’)-IIa) were highly prevalent. In addition, 5 β-lactam resistance genes (blaTEM, blaSHV, blaCMY-2, blaCTX-M, and blaOXA) and 7 quinolone resistance genes (oqxA, oqxB, qnrB, qnrC, qnrD, qnrS, and qeqA) were detected among these isolates. 12 out of 17 virulence genes selected in this study were commonly presented in the chromosomes of tested isolate, with a detection rate of over 80%, including misL, spiA, stn, pagC, iroN, fim, msgA, sopB, prgH, sitC, ttrC, spaN.DiscussionThis study provided a systematical updating on surveillance on prevalence of Salmonella from food animals in China, shedding the light on continued vigilance for Salmonella in food animals.

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Section: Methodsmentioning

confidence: 99%

Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015–2021

Guo

Xiao²,

Wu³

et al. 2023

Front. Microbiol.

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“…PAβN is a widely used efflux-pump inhibitor in Enterobacteriaceae and P. aeruginosa , where a four- or eight-fold reduction in the fluoroquinolone MIC in the PAβN presence is an indicator of an efflux-pump overexpression [ 17 , 18 ]. In our study, 38 strains reduced their ciprofloxacin MIC by more than two-fold in presence of 40 mg/L PAβN ( Table S2 ), 17 of them being ciprofloxacin-resistant strains (MIC reduction range from 4 to > 233 folds).…”

Section: Resultsmentioning

confidence: 99%

Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs

et al. 2022

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Fluoroquinolone resistance and the associated genetic mechanisms were assessed by antimicrobial susceptibility and whole genome sequencing in 56 Pseudomonas aeruginosa strains from human, animal, food and environmental origins. P. aeruginosa PAO1, PA7 and PA14 reference strains were also included in the study. Twenty-two strains (37%) were resistant to, at least, one fluoroquinolone agent. Correlation between the number of changes in GyrA and ParC proteins and the level of fluoroquinolone resistance was observed. Mutations or absence of genes, such as mexZ, mvaT and nalD encoding efflux pumps regulators, were also found in resistant strains. The crpP gene was detected in 43 strains (72.9%; 17 of them non-clinical strains), and coded seven different CrpP variants, including a novel one (CrpP-7). The crpP gene was located in 23 different chromosomal mobile integrative and conjugative elements (ICEs), inserted in two tRNAs integration sites. A great variety of structures was detected in the crpP-ICEs elements, e.g., the fimbriae related cup clusters, the mercury resistance mer operon, the pyocin S5 or S8 bacteriocin encoding genes, and mobilization genes. The location of crpP-like genes in mobilizable ICEs and linked to heavy metal resistance and virulence factors is of significant concern in P. aeruginosa. This work provides a genetic explanation of the fluoroquinolone resistance and crpP-associated pathogenesis of P. aeruginosa from a One-Health approach.

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“…Due to P. aeruginosa infections, minks develop acute, contagious, and fatal hemorrhagic pneumonia as the only known animal species in healthy individuals (Table 1). In Denmark, hemorrhagic pneumonia in minks was frstly reported in 1953 that caused variable mortalities ranging from 1% to 75% [4,5,10,11]. In China, P. aeruginosa pneumonia in minks was frstly reported in 1985 with percentages of mortality ranging from 6% to 54%.…”

Section: Mink Infections Of Pseudomonas Aeruginosamentioning

confidence: 99%

“…Many previous mink studies have worked on the pathology, pathogenesis, serology, antimicrobial resistance, virulence gene, and related diseases of P. aeruginosa to understand its molecular infectious mechanism, to fnd the clinically suitable antibiotics, and to provide the best strategic treatment solutions [10][11][12][13][14][15]. In the meantime, mink vaccinations against P. aeruginosa were developed for clinical treatments, such as bacterin, multicomponent (common protective antigen of P. aeruginosa mixed with toxoids of protease and elastase of the bacillus), and other technological vaccines to protect minks with efective immunity from hemorrhagic pneumonia [5,16,17].…”

Section: Introductionmentioning

confidence: 99%

Overview of Mink Immunity and Resistance to Pseudomonas aeruginosa

Bai,

Wang,

Zhang

et al. 2023

Veterinary Medicine International

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Pseudomonas aeruginosa is one of the most host-susceptible pathogenic bacteria to cause acute and chronic infections in humans and animals. Notably, its infection can especially cause fatal pathogenic infectious pneumonia in minks. Many previous mink studies have investigated the pathology, pathogenesis, serology, antimicrobial resistance, virulence gene, vaccination, and related diseases of P. aeruginosa. However, the relationship of P. aeruginosa infections with mink immunity and resistance is relatively less studied that needs more elaborations. Therefore, we here provide a comprehensive review about mink immunity and resistance to P. aeruginosa infections and the pathogenesis of mink hemorrhagic pneumonia in four major aspects. They include characterization, infection, immunity, and resistance of P. aeruginosa, and their implication and perspective, which aim to contribute the useful and valuable information to further related research and clinical treatment of P. aeruginosa and to avoid the potential fatal hemorrhagic pneumonia spreading.

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Characterization of antimicrobial resistance and virulence genes of Pseudomonas aeruginosa isolated from mink in China, 2011–2020

Cited by 3 publications

References 33 publications

Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015–2021

Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015–2021

Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs

Overview of Mink Immunity and Resistance to Pseudomonas aeruginosa

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