Characterization of antioxidant, α-glucosidase and tyrosinase inhibitors from the rhizomes of Potentilla anserina L. and their structure–activity relationship

Antioxidant, Anti‐Inflammatory, and Antidiabetic Activities of Bioactive Compounds from the Fruits of Livistona chinensis Based on Network Pharmacology Prediction

“…ABTS Assay. The ABTS radical cation (ABTS•+) was prepared by reacting ABTS with potassium persulfate following the method previously described [28]. The ABTS radical cation (ABTS•+) was prepared by mixing with 3 Oxidative Medicine and Cellular Longevity 2.45 mM potassium persulfate and equal volumes of an ABTS solution (7 mM), and then, they were permitted to react in the darkness at room temperature for 12-16 h. Thereafter, the ABTS reagent was diluted with methyl alcohol until reaching an absorbance of 0:70 ± 0:02 units at 734 nm.…”

“…α-Glucosidase Inhibitory Assay. According to the previously published method [28], the α-glucosidase inhibition was assayed using p-nitrophenyl-α-D-glucopyranoside (p-NPG) as the substrate, the release of p-nitrophenol was measured with a microplate reader at 405 nm. Acarbose was used as the positive control.…”

“…This implied that the phenolic hydroxyl group (4-OH) of benzofuran neolignans in FLC was substituted, and the antioxidant capacity was radically decreased. Interestingly, 8-O-4 ′ neolignans (compound [19][20][21][22][23], sesquineolignans (compounds 32-36), and furofuran lignans (compounds [28][29][30][31] were all with at least one phenolic hydroxyl group, and they possessed significant scavenging capacities, especially against ABTS•+. Therefore, the flavonoids 11 Oxidative Medicine and Cellular Longevity with a catechol unit and the lignans with a phenolic hydroxyl group isolated from FLC could be an indelible factor for the antioxidant capacity.…”

Inhibitory Effects of Twenty-Nine Compounds From Potentilla longifolia on Lipid Accumulation and Their Mechanisms in 3T3-L1 Cells

“…The known compounds 4-29 were identified as 2-Oβ-glucopyranosyl -5-methoxy-benzoic acid methyl ester (4) (Kurimoto et al, 2011), 2-β-D-glucopyranosyloxy-4-methoxy-6-hydroxy-isovalero-phenone (5) (Wang et al, 2014a), 1-[(2methylbutyryl) phloroglucinol]-β-D-glucopyranoside (6) (Bohr et al, 2005), 1-(2,4-dihydroxy-6-methoxyphenyl)-3-methyl-1butanone (7) (Wang et al, 2016), 1-O-methyl-2methylphloroglucinol (8) (Arnone et al, 1990), aspidinol D (9) (Wang et al, 2010), 2-methyl-1-(2,4,6-trihydroxy-3methylphenyl)butan-1-one (10) (Wu et al, 2019), 5,7dihydroxyl-3,8,4′-trimethoxylflavone (11) (Liu et al, 2015), 3,8-dimethoxy-5,7,4′-trihydroxyflavone (12) (Gedara et al, 2003), 5,7,4′-trihydroxy-3-methoxy-6-methylflavone (13) (Rabesa and Voirin, 1978), astragalin (14) (Qian et al, 2004), quercetin-3,7-di-O-β-D-glucopyranoside (15) (Huang et al, 2002), hirsutrin (16) (Shu et al, 2011), isorhamnetin (17) (Wang et al, 2014b), trans-tiliroside (18) (Itoh et al, 2009), 6methylapigenin (19) (Wasowski et al, 2002), apigenin (20) (Miao et al, 2008), farrerol (21) (Zhou et al, 2007), 8-demethylfarrerol (22) (Li et al, 2014), pinoresinol-4-O-β-D-glucoside (23) (Cha et al, 2018), schilignan F (24) (Yang et al, 2018), (6R,9R)-3-oxoα-ionol 9-O-β-glucopyranoside (25) (Yang et al, 2010), ursolic acid (26) (Jia et al, 2017), β-sitosterol (27) (Liu and Yang, 2007), 2′, 3′-dihydroxy propylpentadecanoate (28) (Wang et al, 2011), octyl decanoate (29) (Hosseini-Sarvari and Sodagar, 2013) (see Figures 2 and 3).…”

“…Other study also showed that methanol and water extracts of two Potentilla species (P. speciosa L. and P. reptans Willd. ), and biflavanols and quercetin-3-O-α-L-rhamnopyranoside-2''-gallate isolated from P. anserina L. displayed good antioxidant activity (Uysal et al, 2017;Yang et al, 2020). Several tannins and flavonoids have been reported to be present in P. longifolia which was also known as P. viscosa.…”