Characterization of biosensors based on membranes containing a conducting polymer

Battilotti, M.; Colapicchioni, C.; Giannini, Iacopo; Porcelli, Fernando; Campanella, Luigí; Cordatore, M.; Mazzei, Franco; Tomassetti, M.

doi:10.1016/s0003-2670(00)81949-2

Cited by 30 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 A promising approach in this respect is to use an electroactive polymer (conducting polymer or redox polymer) as the ion-to-electron transducer in combination with carrier-based ion-selective membranes. [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] On the basis of their redox and ion-exchange properties, conducting polymers fulfill at least stability condition 1 presented above. [25][26][27][28][29] The fulfilment of stability condition 2 depends on the low-frequency redox capacitance of the conducting polymer, as will be discussed below.…”

mentioning

confidence: 99%

Potential Stability of All-Solid-State Ion-Selective Electrodes Using Conducting Polymers as Ion-to-Electron Transducers

1999

View full text Add to dashboard Cite

Demanding analytical applications such as on-line process analysis and clinical analysis require robust, reliable, and maintenance-free ion sensors of high potential stability. In this work the stability of the electrode potential of all-solid-state ion-selective electrodes using conducting polymers as ion-to-electron transducers is critically evaluated by using chronopotentiometry and electrochemical impedance spectroscopy. This study is focused on the relationship between the potential stability of the electrode and the capacitance of the solid contact where ion-to-electron transduction takes place. The influence of this capacitance on the potential stability of all-solid-state ion-selective electrodes is studied experimentally by using conducting polymer layers of different thickness as solid contacts in potassium ion-selective electrodes based on a solvent polymeric membrane. Because of its excellent environmental stability, the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) is used as a model compound for the solid contact material. Chronopotentiometry is found to be a convenient and fast experimental method to critically evaluate the potential stability of different types of ion-selective electrodes.

show abstract

mentioning

confidence: 99%

Potential Stability of All-Solid-State Ion-Selective Electrodes Using Conducting Polymers as Ion-to-Electron Transducers

1999

View full text Add to dashboard Cite

show abstract

“…In this approach, the conducting polymer may influence also the selectivity of the sensor, depending on the concentration of the conducting polymer in the membrane [221,223]. Inclusion of the conducting polymer polyphenylacetylene in the plasticized PVC membrane was found to improve the low detection limit of the corresponding ISE [224]. Furthermore, acetylene black [225] and PPy [226,227] were included also in plasticized PVC membranes used in the conventional setup with internal filling solution but here the role of the conducting polymers is clearly different from the one discussed in this review.…”

Section: Conducting Polymers As Ion-to-electron Transducersmentioning

confidence: 99%

Potentiometric Ion Sensors Based on Conducting Polymers

2003

View full text Add to dashboard Cite

Conducting polymers (electroactive conjugated polymers, ECPs) have emerged as one of the most promising transducers for chemical sensors. This is related to the unique electrical, electrochemical and optical properties of conjugated polymers that can be used to convert chemical information (concentration, activity, partial pressure) into electrical or optical signals in the solid state. Application of conjugated polymers in potentiometric ion sensors (ionselective electrodes, ISEs) is reviewed.

show abstract

“…In this study, we have used N-ethyl-N -(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) with N-hydroxysuccinimide (NHS) coupling agent as zero length crosslinker which is reported to be noncytotoxic in in vitro [13] and biocompatible in animal studies [14,15]. Urease in immobilized form are also used as biological sensing material for the fabrication of many types of urea sensors including amperometric [16,17], potentiometric [18,19] and optical [20,21]. Those applications require characterization of urease immobilized membranes under static and dynamic conditions.…”

Section: Introductionmentioning

confidence: 99%

Catalytic performances of chemically immobilized urease under static and dynamic conditions: A comparative study

Yürekli

Altınkaya

2011

Journal of Molecular Catalysis B: Enzymatic

View full text Add to dashboard Cite

a b s t r a c tImmobilized urease has been used for direct removal of urea from aqueous solution and as biological sensing material in the preparation of urea biosensors. The former application is carried out under dynamic condition using ultrafiltration membrane either in tubular form or in flat sheet, while the latter is used in static condition. In this study, the performance of chemically immobilized urease on poly(acrylonitrileco-sodium methallyl sulfonate) ultrafiltration membrane was determined under both static and dynamic conditions. Results reveal that the immobilization enhanced the thermal and storage stabilities of urease. The hydraulic permeability of urea solution was not influenced by the addition of enzyme layer. The maximum reaction rate measured under pressure in the ultrafiltration unit was found higher compared to the rate observed just under mixing without any pressure applied. The highest urea conversion was found at the lowest transmembrane pressure and the urea concentration in the feed solution. The catalytic activity of the membrane was completely preserved at the end of 450 min of filtration.

show abstract

Characterization of biosensors based on membranes containing a conducting polymer

Cited by 30 publications

References 7 publications

Potential Stability of All-Solid-State Ion-Selective Electrodes Using Conducting Polymers as Ion-to-Electron Transducers

Potential Stability of All-Solid-State Ion-Selective Electrodes Using Conducting Polymers as Ion-to-Electron Transducers

Potentiometric Ion Sensors Based on Conducting Polymers

Catalytic performances of chemically immobilized urease under static and dynamic conditions: A comparative study

Contact Info

Product

Resources

About