Characterization of biotechnological processes and products using high‐performance liquid chromatography (HPLC). I. Separations of carbohydrates, organic acids and lipids

Abstract

Suitable and optimized chromatographic separation systems for HPLC analyses of mono-, di-and oligomeric carbohydrates, organic acids (e.g. gluconic acid, a-ketoglutaric acid), phospholipids (PE, LPE, LPC, PC, SPH) and neutral lipids (squalene, cholesterol) are demonstrated. Applications of HPLC technique are separation examples of sugars from hydrolyzed starches which were isolated from potatos, calculations of organic acids in fermentation mediums and determinations of neutral lipids and phospholipids which were isolated from microbial biomass. The liquid chromatographic separations are based on self-packed highly efficient (approximately 80000 theoretical plates per meter, N/m) glass columns.The microbial production of single cell proteins (SCP), syntheses of organic acids for the food industry and isolation procedures of enzymes, lipids and other valuable substances including their chromatographic purification are some applications in the field of modern biotechnology. Analytical investigations of these products and their fermentation procedures involve a great variety of tasks. Chromatographic "high-performance techniques" such as HPLC become more and more important for biotechnology because many different substances can be analyzed and preparatively isolated in short times. In most cases derivatization procedures are not necessary and the substances to be analyzed must only be soluble in a liquid phase. This is the reason why 80% of organic compounds known today can be examined by HPLC [ 11.

Pseudo reaction kinetics of organic degradation products in dilute-acid-catalyzed corn stover pretreatment hydrolysates

“…Forty-one reference compounds reflecting a wide range of potential analytes were selected based on previous reports of hydrolysate composition (Alén et al, 1984;Bipp et al, 1997;Bonn and Bobleter, 1984;Bonn et al, 1988;Burtscher et al, 1987;DiNardo and Larson, 1994;Fenske et al, 1998;Gey, 1988;Karagöz et al, 2004;Klinke et al, 2002Klinke et al, , 2004Lapierre et al, 1983;Luo et al, 2002;Niemelä, 1988;Niemelä and Sjöström, 1986;Pecina et al, 1986;Persson et al, 2002). Reference standards and calibrators were prepared from the group of purchased reference standards using water as the diluent.…”

Application of a Portable Multi-Analyte Biosensor for Organic Acid Determination in Silage

“…For successful control of ensiling or other fermentation processes, therefore, continuous monitoring of different intermediates (like ethanol, formate, d -lactate, and l -lactate) is mandatory. The most common analytical techniques for quantification of these analytes are high performance liquid chromatography [ 9 , 10 ], gas chromatography [ 11 ], and UV-VIS spectrophotometry. These methods generally possess high accuracy and sensitivity, but also may be associated with some potential drawbacks.…”