Characterization of Boar Seminal Plasma, Vesicular Secretion and Epididymal Plasma Proteins by Gel Disc Electrophoresis and Isoelectric Focusing on Polyacrylamide

Abstract

Boar seminal plasma, vesicular secretion and epididymal plasma proteins were studied by gel disc electrophoresis at pH 4\m=.\5and 8\m=.\6and by isoelectric focusing in tubes and on plates using ampholines of pH range 3 to 10.The number of protein bands obtained in the gel disc electrophoresis was fifteen to twenty for all the samples. A similarity between the proteins of the seminal plasma and the vesicular secretion was greater at pH 4\m=.\5than at pH 8\m=.\6.The epididymal plasma showed a different separation picture.The better resolution of the isoelectric focusing revealed a greater number of proteins. Similar protein patterns were found for the vesicular secretion and the seminal plasma in the basic part of the pH range, where the majority of the proteins of these fluids are found. The proteins of the epididymal plasma, on the other hand, possess more neutral isoelectric points and their contribution to the seminal plasma is smaller than that of the vesicular secretion. Only a few of the epididymal plasma proteins and of the minor vesicular secretion proteins could be found in the neutral and acidic regions of the seminal plasma. This is largely the result of the dilution of these accessory secretions occurring during ejaculation.

Characterization of the proteomes associating with three distinct membrane raft sub‐types in murine sperm

“…This supports the notion that electrostatic interactions between proteins and other membrane components play important roles in a variety of cellular processes [52]. Although membranes used for our study were never exposed to seminal plasma, it is known that basic proteins are highly abundant in seminal plasma [53], and at least several of these interact directly with components of the sperm plasma membrane after ejaculation [54, 55]. These interactions have physiological importance in terms of acquisition and maintenance of motility [56–58], or prevention of activation of signaling cascades involved in capacitation [55, 59].…”

The Split Ejaculate of the Boar: Contributions of the Epididymides and Seminal Vesicles

“…1) was eighteen, and twelve or more of these could be detected in the sperm-rich fraction (Plate 1, Figs 3 and 4). This number was greater than the three proteins obtained by Einarsson et al (1970) using paper electrophoresis at pH 8-6, a pH shown by Lavon & Boursnell (1971) to be unsuitable for boar SP, VS and EP protein differentiation.…”

“…Instruments, South Croydon) which were prepared and used as described by Lavon & Boursnell (1971) and by Lavon (1972). The correlation coefficients between the various parameters were calculated according to the method described by Bliss (1967).…”

“…In addition, Lavon & Boursnell (1971), Lavon, Boursnell & Briggs (1972) Boursnell, Hartree & Briggs (1970). Epididymal semen from freshly excised epididymides from the same carcasses was collected by the method of Lasley & Bogart (1944) as described by Walton (1957).…”

Differences in the Electrophoretic Characteristics of Bovine Rete Testis Fluid and Plasma From the Cauda Epididymidis

“…The electrophoretic pattern of CEP has been reported for rams (Alumot et al, 1971) and boars (Einarsson, Crabo & Ekman, 1970;Lavon & Boursnell, 1971). The broad spectrum of proteins detected in bovine CEP was thus expected.…”