Characterization of Dental Nociceptive Neurons

Kim, H.Y.; Chung, Grace H.; Jo, Hyun Jung; Kim, Y.S.; Bae, Yong Chul; Jung, Sung Jun; Kim, J.-S.; Oh, Seog Bae

doi:10.1177/0022034511399906

Cited by 58 publications

(64 citation statements)

References 37 publications

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“…In rats, labeling of IB4 in TG sections showed that less than 5% of retrogradely labeled dental afferents are IB4-positive (Fried et al, 1989;Yang et al, 2002;Kvinnsland et al, 2004;Gibbs et al, 2011). In contrast, the percentages of IB4-positive pulpal afferents in cultured dental afferents were as high as 75% (Park et al, 2006;Kim et al, 2011). The source of such a discrepancy is not clear.…”

Section: Discussionmentioning

confidence: 98%

Projection of Non-peptidergic Afferents to Mouse Tooth Pulp

2012

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrAct A large proportion of pulpal nociceptors are known to contain neuropeptides such as CGRP. However, the projection of non-peptidergic nociceptors to tooth pulp is controversial. Recently, the nonpeptidergic subset of nociceptors has been implicated in mechanical pain in the skin. Since mechanical irritation of pulpal nociceptors is critical for evoking tooth pain under pathophysiological conditions, we investigated whether the non-peptidergic afferents project to tooth pulp as potential mechanotransducing afferents. For clear visualization of the non-peptidergic afferents, we took advantage of a recently generated knock-in mouse model in which an axonal tracer, farnesylated green fluorescence protein (GFP), is expressed from the locus of a sensory neuron-specific gene, Mrgprd. In the trigeminal ganglia (TG), we demonstrated that GFP is exclusively expressed in afferents binding to isolectin B4 (IB4), a neurochemical marker of non-peptidergic nociceptors, but is rarely co-localized with CGRP. Retrograde labeling of pulpal afferents demonstrated that a low proportion of pulpal afferents was co-localized with GFP. Immunohistochemical detection of the axonal tracer revealed that GFP-positive afferent terminals were densely projected into the tooth pulp. These results provide convincing evidence that non-peptidergic nociceptors are projected into the tooth pulp and suggest a potential role for these afferents in tooth pain.

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Section: Discussionmentioning

confidence: 98%

Projection of Non-peptidergic Afferents to Mouse Tooth Pulp

2012

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“…A combination of molecular [single-cell reverse-transcription polymerase chain reaction (RT-PCR)], immunohistochemical, and functional (patch clamping) studies revealed that most (up to 85%) trigeminal ganglion neurons innervating the tooth pulp express TRPV1 (Park et al, 2006;Kim et al, 2011b). Importantly, these neurons respond to noxious heat (and also capsaicin) with elevated intracellular calcium levels (Chaudhary et al, 2001).…”

Section: Transient Receptor Potential Channels: Acquired Diseasesmentioning

confidence: 99%

Transient Receptor Potential Channels as Drug Targets: From the Science of Basic Research to the Art of Medicine

2014

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“…Les canaux thermo-TRP sont représentés par les canaux TRPV (vanilloïde), TRPA (ankyrine) et TRPM (mélastatine). Les canaux TRPV1 et TRPV2, activés par la chaleur, ainsi que les canaux TRPA1 et TRPM8, activés par le froid, ont été identifiés dans les corps cellulaires des neurones trigéminaux à l'origine de l'innervation pulpaire [6]. TRPV1 et TRPV2 sont principalement exprimés par les fibres myélinisées.…”

Section: Diversité Des Afférences Dentaires Primairesunclassified