Characterization of Dielectric Barrier Discharge (DBD) on Mouse and Histological Evaluation of the Plasma‐Treated Tissue

Rajasekaran, Priyadarshini; Opländer, Christian; Hoffmeister, Dennis; Bibinov, Nikita; Suschek, Christoph V.; Wandke, Dirk; Awakowicz, Peter

doi:10.1002/ppap.201000122

Cited by 44 publications

(27 citation statements)

References 30 publications

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“…Under these conditions, the discharge can be described as spatially and temporally distributed, transient filamentary microdischarges with a homogeneous background. 19,20 Inside the plasma, a cocktail of reactive species is produced, including RNOS, UV-photons and ionic species, which can all affect the skin chemistry. Hence, it is important to identify key reaction products.…”

Section: Methodsmentioning

confidence: 99%

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Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy

et al. 2015

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Using infrared and Raman microspectroscopy, the authors examined the interaction of cold atmospheric plasma with the skin's built-in protective cushion, the outermost skin layer stratum corneum. Following a spectroscopic analysis, the authors could identify four prominent chemical alterations caused by plasma treatment: (1) oxidation of disulfide bonds in keratin leading to a generation of cysteic acid; (2) formation of organic nitrates as well as (3) of new carbonyl groups like ketones, aldehydes and acids; and (4) reduction of double bonds in the lipid matter lanolin, which resembles human sebum. The authors suggest that these generated acidic and NO-containing functional groups are the source of an antibacterial and regenerative environment at the treatment location of the stratum corneum. Based upon the author's results, the authors propose a mechanistic view of how cold atmospheric plasmas could modulate the skin chemistry to produce positive long-term effects on wound healing: briefly, cold atmospheric plasmas have the potential to transform the skin itself into a therapeutic resource.

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Section: Methodsmentioning

confidence: 99%

“…This type of CAP has great potential for medical applications, because it operates in air, without the need for externally supplied process gases. 19 …”

Section: Introductionmentioning

confidence: 97%

Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy

et al. 2015

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“…These can be calculated applying temporary and space averaged plasma parameters. The influence of inhomogeneity of the plasma parameters on calculated excitation (or dissociation) rates by application of OES diagnostics was studied in [9], [22], [6], and a good agreement between space resolved and averaged excitation rates was found in most cases. In emission spectrum of DBD in air, the vibrational bands of 'second positive' (N 2 (C-B)) and 'first negative' (N 2 + (B-X)) systems of nitrogen are observed.…”

Section: Diagnostics and Plasma Characterizationmentioning

confidence: 99%

Characterization of transient discharges under atmospheric-pressure conditions applying nitrogen photoemission and current measurements

Keller

Rajasekaran

Bibinov

et al. 2012

J. Phys. D: Appl. Phys.

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Abstract.The plasma parameters such as electron distribution function and electron density of three atmospheric-pressure transient discharges namely filamentary and homogeneous dielectric barrier discharges in air, and the spark discharge of argon plasma coagulation (APC) system are determined. A combination of numerical simulation as well as diagnostic methods including current measurement and optical emission spectroscopy (OES) based on nitrogen emissions is used. The applied methods supplement each other and resolve problems, which arise when these methods are used individually. Nitrogen is used as sensor gas and is admixed in low amount to argon for characterizing the APC discharge. Both direct and stepwise electron impact excitation of nitrogen emissions are included in the plasma-chemical model applied for characterization of these transient discharges using OES where ambiguity arises in the determination of plasma parameters at specific discharge conditions. It is shown that the measured current solves this problem by providing additional information useful for the determination of discharge-specific plasma parameters.

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“…The negligible effects of electrical field or flowing current have been reported as well by others who used plasma to treat mice or even human patients. 11,25 …”

Section: Resultsmentioning

confidence: 99%

Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model

Xiong

Wang

Yin

et al. 2018

Clinical Plasma Medicine

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Cold plasma generated at atmospheric pressure has attracted intense interest in biomedical applications, particularly as an antimicrobial treatment. Here we report the therapeutic effect of humidified cold argon plasma on allergic contact dermatitis (ACD) in a mouse model. Treatment was carried out with different gas compositions: argon gas containing small amounts of either N2, O2, or H2O. The best effect was obtained using humid plasma (H2O addition), where the ACD symptoms decreased after one or two 1-min plasma treatments. Even for severe ACD with ulcers and crust formation, the humid plasma-treated mice recovered faster than the control group. Histopathological analysis by H&E-staining showed enhanced epithelialization with formation of collagen and hair follicles in the affected skin after humid plasma exposure. The therapeutic ability of the humid argon plasma discharge was proposed to be induced by reactive oxygen species (HxOy) transported from the discharge zone, which are adhesive and accumulate on the skin surface, penetrating the subcutis to eliminate inflammation. However, in treatments using plasma with addition of oxygen or nitrogen (without water) the active gaseous species are blocked due to poor adhesion to and penetration into the dry ACD skin, with correspondingly poor treatment effects. The enhanced in vivo healing in ACD mice indicate the non-thermal humid plasma could be a potential alternative approach for therapy of ACD and other inflammatory skin diseases.

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Characterization of Dielectric Barrier Discharge (DBD) on Mouse and Histological Evaluation of the Plasma‐Treated Tissue

Cited by 44 publications

References 30 publications

Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy

Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy

Characterization of transient discharges under atmospheric-pressure conditions applying nitrogen photoemission and current measurements

Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model

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