Characterization of different RP-HPLC columns by a gradient elution technique

Sándi, Ákos; Bede, Á.; Szepesy, L.; Rippel, Géza

doi:10.1007/bf02505562

Cited by 56 publications

(22 citation statements)

References 48 publications

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“…Several research groups published original research articles in this field and some reviews were also presented recently [24,32,38,[47][48][49][50][51][52][53][54][55]. The research groups used different parameters for the characterization of columns and different chemometric methods were used for data evaluation.…”

Section: Introductionmentioning

confidence: 99%

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Németh

Haghedooren

Noszál

et al. 2008

Journal of Chemometrics

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Selecting a suitable reversed phase liquid chromatographic (RP-LC) column for a particular separation is complicated. More than 600 brands of C 18 columns are available on the market. Therefore, determining the characteristics influencing the selectivity of the different stationary phases is necessary. In this paper, three different methods to characterize RP-LC columns are compared. Hoogmartens et al. developed a column characterization system based on test methods from literature, which were reduced with principal component analysis (PCA), and led to four final chromatographic parameters. The second method studied is this of Euerby et al. who developed a column classification system using six parameters, based on the method of Tanaka and also applying PCA. The third method, developed by Snyder et al. is different from the other two. It uses five parameters, calculated by multiple linear regression from the retention data of 18 solutes. The suitability of the column classification systems was examined on pharmaceutical separations. It seems that all three methods can facilitate the selection of a suitable column for an analysis.

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Section: Introductionmentioning

confidence: 99%

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Németh

Haghedooren

Noszál

et al. 2008

Journal of Chemometrics

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“…One can easily understand that a major problem facing the chromatographer is to effectively choose the appropriate reversed-phase packing for a particular separation from the wide range of commercially offered materials. This is why, because of the variety and number of packings, a fast procedure is needed to evaluate the columns [3,4]. However, as demonstrated by Sander and Wise, there is no unified procedure described in the literature for the evaluation and characterisation of reversed-phase (RP) columns with different pore sizes (surface area) and types of ligand.…”

Section: Column Testsmentioning

confidence: 99%

Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II

et al. 2001

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Reversed-phase chromatography is the method of choice for the analysis of basic compounds. However, because of the problems encountered with these compounds, “base deactivated” stationary phases have been developed. This review focuses on a major problem encountered by the chromatographer which is to effectively choose, for a particular separation, the appropriate reversed-phase packing. Often, columns of the same type but provided by various manufacturers vary in their chromatographic behaviour. It is known that differences in the purity of column packings, in the properties of silica gel supports and the density of silanols on the surface can be responsible for chromatographic differences. Unfortunately, in the case of “base deactivated” columns there is no universal test and chromatographic effects are compound dependent. The aim of this review is to describe the various chromatographic tests proposed in the literature for the differentiation of the many stationary phases on the market

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“…Until now, there exists no universally accepted chromatographic test to choose an appropriate packing material for a particular separation problem [1]. In reversed-phase chromatography, many descriptors can give certain information to estimate the chromatographic behavior of the stationary phases; i.e.…”

Section: Introductionmentioning

confidence: 99%

Preparation and chromatographic performance of calix[4]crown‐5 macrocycle‐bonded silica stationary phase

Erdemir

2011

J of Separation Science

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A new calix[4]crown-5 macrocycle-bonded silica stationary phase (CL-CIMS) was prepared and applied at the same time to develop a chromatographic procedure to separate aromatic amines, phenols and drugs in this study. The chromatographic behaviors of the prepared stationary phase for these analytes were studied and compared with those of ODS (octadecylsilane). The effect of organic modifier content and pH of the mobile phase on retention and selectivity of these compounds were investigated. Some aromatic amines, phenols or drugs on CL-CIMS were successfully separated. The results show that CL-CIMS exhibits high selectivities for the above analytes in high aqueous mobile phases and a bright prospect in routine, fast separation of aromatic amines, phenols and drug compounds. From chromatographic data, it can be concluded that hydrophobic interaction is mainly responsible for the retention behavior as well as hydrogen-bonding interaction, π-π and dipole-dipole interaction.

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Characterization of different RP-HPLC columns by a gradient elution technique

Cited by 56 publications

References 48 publications

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Three methods to characterize reversed phase liquid chromatographic columns applied to pharmaceutical separations

Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II

Preparation and chromatographic performance of calix[4]crown‐5 macrocycle‐bonded silica stationary phase

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